File Download
There are no files associated with this item.
Links for fulltext
(May Require Subscription)
- Publisher Website: 10.1016/j.archoralbio.2018.04.019
- Scopus: eid_2-s2.0-85047070569
- PMID: 29763780
- WOS: WOS:000436915400010
- Find via
Supplementary
- Citations:
- Appears in Collections:
Article: Polymorphonuclear neutrophil integrity and functionality are preserved when exposed to saliva
Title | Polymorphonuclear neutrophil integrity and functionality are preserved when exposed to saliva |
---|---|
Authors | |
Keywords | Neutrophils Oral health Phagocytosis Reactive oxygen species Saliva |
Issue Date | 2018 |
Citation | Archives of Oral Biology, 2018, v. 92, p. 68-74 How to Cite? |
Abstract | Objective: Polymorphonuclear neutrophils (PMNs) are the most abundant innate immune cells and are also important effectors in the maintenance of oral health. However, little is known about the effects of saliva on the PMN. We therefore aimed to investigate the effect of saliva on the PMNs’ morphology and functioning. Design: Effect of saliva on the membrane integrity of PMNs isolated from blood was evaluated with FACS using Annexin V (apoptosis marker) and propidum iodide (membrane integrity marker). The effect on cell morphology was examined using transmission electron imaging. Binding and phagocytosis of the oral bacterium Fusobacterium nucleatum by PMNs was analysed by FACS. Reactive oxygen species (ROS) production was measured with chemiluminescence. Results: Incubation with saliva for 60 min had no detectable effects on the membrane integrity or the morphology of PMNs. In contrast, preincubation of F. nucleatum with saliva inhibited its subsequent interaction with PMNs, resulting in a diminished production of ROS. Conclusions: Saliva does not impair the function of PMNs. However, interaction of salivary components with F. nucleatum may affect their recognition by PMNs resulting in a diminished functional response. |
Persistent Identifier | http://hdl.handle.net/10722/336938 |
ISSN | 2023 Impact Factor: 2.2 2023 SCImago Journal Rankings: 0.562 |
ISI Accession Number ID |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Rijkschroeff, Patrick | - |
dc.contributor.author | Gunput, Sabrina T.G. | - |
dc.contributor.author | Ligtenberg, Antoon J.M. | - |
dc.contributor.author | Veerman, Enno C.I. | - |
dc.contributor.author | Loos, Bruno G. | - |
dc.contributor.author | Nicu, Elena A. | - |
dc.date.accessioned | 2024-02-29T06:57:34Z | - |
dc.date.available | 2024-02-29T06:57:34Z | - |
dc.date.issued | 2018 | - |
dc.identifier.citation | Archives of Oral Biology, 2018, v. 92, p. 68-74 | - |
dc.identifier.issn | 0003-9969 | - |
dc.identifier.uri | http://hdl.handle.net/10722/336938 | - |
dc.description.abstract | Objective: Polymorphonuclear neutrophils (PMNs) are the most abundant innate immune cells and are also important effectors in the maintenance of oral health. However, little is known about the effects of saliva on the PMN. We therefore aimed to investigate the effect of saliva on the PMNs’ morphology and functioning. Design: Effect of saliva on the membrane integrity of PMNs isolated from blood was evaluated with FACS using Annexin V (apoptosis marker) and propidum iodide (membrane integrity marker). The effect on cell morphology was examined using transmission electron imaging. Binding and phagocytosis of the oral bacterium Fusobacterium nucleatum by PMNs was analysed by FACS. Reactive oxygen species (ROS) production was measured with chemiluminescence. Results: Incubation with saliva for 60 min had no detectable effects on the membrane integrity or the morphology of PMNs. In contrast, preincubation of F. nucleatum with saliva inhibited its subsequent interaction with PMNs, resulting in a diminished production of ROS. Conclusions: Saliva does not impair the function of PMNs. However, interaction of salivary components with F. nucleatum may affect their recognition by PMNs resulting in a diminished functional response. | - |
dc.language | eng | - |
dc.relation.ispartof | Archives of Oral Biology | - |
dc.subject | Neutrophils | - |
dc.subject | Oral health | - |
dc.subject | Phagocytosis | - |
dc.subject | Reactive oxygen species | - |
dc.subject | Saliva | - |
dc.title | Polymorphonuclear neutrophil integrity and functionality are preserved when exposed to saliva | - |
dc.type | Article | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1016/j.archoralbio.2018.04.019 | - |
dc.identifier.pmid | 29763780 | - |
dc.identifier.scopus | eid_2-s2.0-85047070569 | - |
dc.identifier.volume | 92 | - |
dc.identifier.spage | 68 | - |
dc.identifier.epage | 74 | - |
dc.identifier.eissn | 1879-1506 | - |
dc.identifier.isi | WOS:000436915400010 | - |