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- Publisher Website: 10.1002/anie.202313096
- Scopus: eid_2-s2.0-85173444290
- PMID: 37728515
- WOS: WOS:001076288000001
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Article: Synthetic Membraneless Droplets for Synaptic-Like Clustering of Lipid Vesicles
Title | Synthetic Membraneless Droplets for Synaptic-Like Clustering of Lipid Vesicles |
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Authors | |
Keywords | Biomolecular Condensates Lipid Vesicles Liquid-Liquid Phase Separation Membraneless Organelles Synthetic Cell |
Issue Date | 2023 |
Citation | Angewandte Chemie - International Edition, 2023, v. 62, n. 45, article no. e202313096 How to Cite? |
Abstract | In eukaryotic cells, the membraneless organelles (MLOs) formed via liquid-liquid phase separation (LLPS) are found to interact intimately with membranous organelles (MOs). One major mode is the clustering of MOs by MLOs, such as the formation of clusters of synaptic vesicles at nerve terminals mediated by the synapsin-rich MLOs. Aqueous droplets, including complex coacervates and aqueous two-phase systems, have been plausible MLO-mimics to emulate or elucidate biological processes. However, neither of them can cluster lipid vesicles (LVs) like MLOs. In this work, we develop a synthetic droplet assembled from a combination of two different interactions underlying the formation of these two droplets, namely, associative and segregative interactions, which we call segregative-associative (SA) droplets. The SA droplets cluster and disperse LVs recapitulating the key functional features of synapsin condensates, which can be attributed to the weak electrostatic interaction environment provided by SA droplets. This work suggests LLPS with combined segregative and associative interactions as a possible route for synaptic clustering of lipid vesicles and highlights SA droplets as plausible MLO-mimics and models for studying and mimicking related cellular dynamics. |
Persistent Identifier | http://hdl.handle.net/10722/336949 |
ISSN | 2023 Impact Factor: 16.1 2023 SCImago Journal Rankings: 5.300 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Li, Qingchuan | - |
dc.contributor.author | Song, Qingchun | - |
dc.contributor.author | Guo, Wei | - |
dc.contributor.author | Cao, Yang | - |
dc.contributor.author | Cui, Xinyu | - |
dc.contributor.author | Chen, Dairong | - |
dc.contributor.author | Shum, Ho Cheung | - |
dc.date.accessioned | 2024-02-29T06:57:39Z | - |
dc.date.available | 2024-02-29T06:57:39Z | - |
dc.date.issued | 2023 | - |
dc.identifier.citation | Angewandte Chemie - International Edition, 2023, v. 62, n. 45, article no. e202313096 | - |
dc.identifier.issn | 1433-7851 | - |
dc.identifier.uri | http://hdl.handle.net/10722/336949 | - |
dc.description.abstract | In eukaryotic cells, the membraneless organelles (MLOs) formed via liquid-liquid phase separation (LLPS) are found to interact intimately with membranous organelles (MOs). One major mode is the clustering of MOs by MLOs, such as the formation of clusters of synaptic vesicles at nerve terminals mediated by the synapsin-rich MLOs. Aqueous droplets, including complex coacervates and aqueous two-phase systems, have been plausible MLO-mimics to emulate or elucidate biological processes. However, neither of them can cluster lipid vesicles (LVs) like MLOs. In this work, we develop a synthetic droplet assembled from a combination of two different interactions underlying the formation of these two droplets, namely, associative and segregative interactions, which we call segregative-associative (SA) droplets. The SA droplets cluster and disperse LVs recapitulating the key functional features of synapsin condensates, which can be attributed to the weak electrostatic interaction environment provided by SA droplets. This work suggests LLPS with combined segregative and associative interactions as a possible route for synaptic clustering of lipid vesicles and highlights SA droplets as plausible MLO-mimics and models for studying and mimicking related cellular dynamics. | - |
dc.language | eng | - |
dc.relation.ispartof | Angewandte Chemie - International Edition | - |
dc.subject | Biomolecular Condensates | - |
dc.subject | Lipid Vesicles | - |
dc.subject | Liquid-Liquid Phase Separation | - |
dc.subject | Membraneless Organelles | - |
dc.subject | Synthetic Cell | - |
dc.title | Synthetic Membraneless Droplets for Synaptic-Like Clustering of Lipid Vesicles | - |
dc.type | Article | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1002/anie.202313096 | - |
dc.identifier.pmid | 37728515 | - |
dc.identifier.scopus | eid_2-s2.0-85173444290 | - |
dc.identifier.volume | 62 | - |
dc.identifier.issue | 45 | - |
dc.identifier.spage | article no. e202313096 | - |
dc.identifier.epage | article no. e202313096 | - |
dc.identifier.eissn | 1521-3773 | - |
dc.identifier.isi | WOS:001076288000001 | - |