Novel tio2 hemisphere surface matrices determine the osteogenic differentiation and mineralization of pre-osteoblastic cells

Abstract

Statement of Purpose: Osseointegration at the bone-implant interface is of paramount importance for the success of orthopaedic implantations and their durability in long-term. Among a number of studies focused on modifying biomaterial physical and chemical properties, the design of surface topography still plays a significant role due to the ease of fabrication and clinical translation [1]. This approach also enables stable physical cue for cell-to-cell interaction, thereby leading to the up-regulations of cells towards osteoblastic differentiation and mineralization [2]. However, most of the studies were not just adopted physical modification, but also came along with surface treatment chemically. Therefore, the superior biological performance of modified biomaterials could hardly attribute to the surface topography cues alone. In this study, a series of TiO2 hemisphere patterns ranging from nanoscale to microscale were prepared on titanium surface by using an interfacial nanosphere lithography method. This study aims to investigate the underlying correlations between surface topography and osteogenic differentiation capability of pre-osteoblasts.