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Article: Tailoring the multiscale mechanics of tunable decellularized extracellular matrix (dECM) for wound healing through immunomodulation

TitleTailoring the multiscale mechanics of tunable decellularized extracellular matrix (dECM) for wound healing through immunomodulation
Authors
KeywordsDecellularized extracellular matrix
Freeze-thaw treatment
Immunomodulation
Macrophage polarization
Mechanotransduction
Multiscale mechanics
Wound healing
Issue Date17-May-2023
PublisherElsevier
Citation
Bioactive Materials, 2023, v. 28, p. 95-111 How to Cite?
AbstractWith the discovery of the pivotal role of macrophages in tissue regeneration through shaping the tissue immune microenvironment, various immunomodulatory strategies have been proposed to modify traditional biomaterials. Decellularized extracellular matrix (dECM) has been extensively used in the clinical treatment of tissue injury due to its favorable biocompatibility and similarity to the native tissue environment. However, most reported decellularization protocols may cause damage to the native structure of dECM, which undermines its inherent advantages and potential clinical applications. Here, we introduce a mechanically tunable dECM prepared by optimizing the freeze-thaw cycles. We demonstrated that the alteration in micromechanical properties of dECM resulting from the cyclic freeze-thaw process contributes to distinct macrophage-mediated host immune responses to the materials, which are recently recognized to play a pivotal role in determining the outcome of tissue regeneration. Our sequencing data further revealed that the immunomodulatory effect of dECM was induced via the mechnotrasduction pathways in macrophages. Next, we tested the dECM in a rat skin injury model and found an enhanced micromechanical property of dECM achieved with three freeze-thaw cycles significantly promoted the M2 polarization of macrophages, leading to superior wound healing. These findings suggest that the immunomodulatory property of dECM can be efficiently manipulated by tailoring its inherent micromechanical properties during the decellularization process. Therefore, our mechanics-immunomodulation-based strategy provides new insights into the development of advanced biomaterials for wound healing.
Persistent Identifierhttp://hdl.handle.net/10722/336999
ISSN
2023 Impact Factor: 18.0
2023 SCImago Journal Rankings: 3.466
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLuo, P-
dc.contributor.authorHuang, RX-
dc.contributor.authorWu, Y-
dc.contributor.authorLiu, XC-
dc.contributor.authorShan, ZJ-
dc.contributor.authorGong, L-
dc.contributor.authorDeng, SD-
dc.contributor.authorLiu, HW-
dc.contributor.authorFang, JH-
dc.contributor.authorWu, SY-
dc.contributor.authorWu, XY-
dc.contributor.authorLiu, Q-
dc.contributor.authorChen, ZT-
dc.contributor.authorYeung, KWK-
dc.contributor.authorQiao, W-
dc.contributor.authorChen, SC-
dc.contributor.authorChen, ZF -
dc.date.accessioned2024-03-11T10:17:14Z-
dc.date.available2024-03-11T10:17:14Z-
dc.date.issued2023-05-17-
dc.identifier.citationBioactive Materials, 2023, v. 28, p. 95-111-
dc.identifier.issn2452-199X-
dc.identifier.urihttp://hdl.handle.net/10722/336999-
dc.description.abstractWith the discovery of the pivotal role of macrophages in tissue regeneration through shaping the tissue immune microenvironment, various immunomodulatory strategies have been proposed to modify traditional biomaterials. Decellularized extracellular matrix (dECM) has been extensively used in the clinical treatment of tissue injury due to its favorable biocompatibility and similarity to the native tissue environment. However, most reported decellularization protocols may cause damage to the native structure of dECM, which undermines its inherent advantages and potential clinical applications. Here, we introduce a mechanically tunable dECM prepared by optimizing the freeze-thaw cycles. We demonstrated that the alteration in micromechanical properties of dECM resulting from the cyclic freeze-thaw process contributes to distinct macrophage-mediated host immune responses to the materials, which are recently recognized to play a pivotal role in determining the outcome of tissue regeneration. Our sequencing data further revealed that the immunomodulatory effect of dECM was induced via the mechnotrasduction pathways in macrophages. Next, we tested the dECM in a rat skin injury model and found an enhanced micromechanical property of dECM achieved with three freeze-thaw cycles significantly promoted the M2 polarization of macrophages, leading to superior wound healing. These findings suggest that the immunomodulatory property of dECM can be efficiently manipulated by tailoring its inherent micromechanical properties during the decellularization process. Therefore, our mechanics-immunomodulation-based strategy provides new insights into the development of advanced biomaterials for wound healing.-
dc.languageeng-
dc.publisherElsevier-
dc.relation.ispartofBioactive Materials-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectDecellularized extracellular matrix-
dc.subjectFreeze-thaw treatment-
dc.subjectImmunomodulation-
dc.subjectMacrophage polarization-
dc.subjectMechanotransduction-
dc.subjectMultiscale mechanics-
dc.subjectWound healing-
dc.titleTailoring the multiscale mechanics of tunable decellularized extracellular matrix (dECM) for wound healing through immunomodulation-
dc.typeArticle-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1016/j.bioactmat.2023.05.011-
dc.identifier.scopuseid_2-s2.0-85159190132-
dc.identifier.volume28-
dc.identifier.spage95-
dc.identifier.epage111-
dc.identifier.eissn2452-199X-
dc.identifier.isiWOS:000995866700001-
dc.identifier.issnl2452-199X-

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