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Article: Polymorphism analysis of pfmdr1 gene in Plasmodium falciparum isolates 11 years post-adoption of artemisinin-based combination therapy in Saudi Arabia
Title | Polymorphism analysis of pfmdr1 gene in Plasmodium falciparum isolates 11 years post-adoption of artemisinin-based combination therapy in Saudi Arabia |
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Authors | |
Issue Date | 11-Jan-2022 |
Publisher | Nature Research |
Citation | Scientific Reports, 2022, v. 12, n. 1 How to Cite? |
Abstract | A total of 227 Plasmodium falciparum isolates from Jazan region, southwestern Saudi Arabia were amplified for the P. falciparum multi-drug resistance 1 (pfmdr1) gene to detect point mutations 11 years after the introduction of artemisinin-based combination therapy (ACT) in Saudi Arabia. The pfmdr1 86Y mutation was found in 11.5% (26/227) of the isolates while the N86 wild allele was detected in 88.5%. Moreover, 184F point mutations dominated (86.3%) the instances of pfmdr1 polymorphism while no mutation was observed at codons 1034, 1042 and 1246. Three pfmdr1 haplotypes were identified, NFSND (74.9%), NYSND (13.7%) and YFSND (11.4%). Associations of the prevalence of 86Y mutation and YFSND haplotype with participants’ nationality, residency and parasitaemia level were found to be significant (P < 0.05). The findings revealed significant decline in the prevalence of the pfmdr1 86Y mutation in P. falciparum isolates from Jazan region over a decade after the implementation of ACT treatment. Moreover, the high prevalence of the NFSND haplotype might be indicative of the potential emergence of CQ-sensitive but artemether-lumefantrine-resistant P. falciparum strains since the adoption of ACT. Therefore, continuous monitoring of the molecular markers of antimalarial drug resistance in Jazan region is highly recommended. |
Persistent Identifier | http://hdl.handle.net/10722/337256 |
ISSN | 2023 Impact Factor: 3.8 2023 SCImago Journal Rankings: 0.900 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Al-Mekhlafi, HM | - |
dc.contributor.author | Madkhali, AM | - |
dc.contributor.author | Abdulhaq, AA | - |
dc.contributor.author | Atroosh, WM | - |
dc.contributor.author | Ghzwani, AH | - |
dc.contributor.author | Zain, KA | - |
dc.contributor.author | Ghailan, KY | - |
dc.contributor.author | Hamali, HA | - |
dc.contributor.author | Mobarki, AA | - |
dc.contributor.author | Alharazi, TH | - |
dc.contributor.author | Eisa, ZM | - |
dc.contributor.author | Lau, YL | - |
dc.date.accessioned | 2024-03-11T10:19:17Z | - |
dc.date.available | 2024-03-11T10:19:17Z | - |
dc.date.issued | 2022-01-11 | - |
dc.identifier.citation | Scientific Reports, 2022, v. 12, n. 1 | - |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.uri | http://hdl.handle.net/10722/337256 | - |
dc.description.abstract | <p>A total of 227 <em>Plasmodium falciparum</em> isolates from Jazan region, southwestern Saudi Arabia were amplified for the <em>P. falciparum multi-drug resistance 1</em> (<em>pfmdr1</em>) gene to detect point mutations 11 years after the introduction of artemisinin-based combination therapy (ACT) in Saudi Arabia. The <em>pfmdr1</em> 86<strong>Y</strong> mutation was found in 11.5% (26/227) of the isolates while the N86 wild allele was detected in 88.5%. Moreover, 184<strong>F</strong> point mutations dominated (86.3%) the instances of <em>pfmdr1</em> polymorphism while no mutation was observed at codons 1034, 1042 and 1246. Three <em>pfmdr1</em> haplotypes were identified, N<strong>F</strong>SND (74.9%), NYSND (13.7%) and <strong>YF</strong>SND (11.4%). Associations of the prevalence of 86<strong>Y</strong> mutation and <strong>YF</strong>SND haplotype with participants’ nationality, residency and parasitaemia level were found to be significant (<em>P</em> < 0.05). The findings revealed significant decline in the prevalence of the <em>pfmdr1</em> 86<strong>Y</strong> mutation in <em>P. falciparum</em> isolates from Jazan region over a decade after the implementation of ACT treatment. Moreover, the high prevalence of the N<strong>F</strong>SND haplotype might be indicative of the potential emergence of CQ-sensitive but artemether-lumefantrine-resistant <em>P. falciparum</em> strains since the adoption of ACT. Therefore, continuous monitoring of the molecular markers of antimalarial drug resistance in Jazan region is highly recommended.</p> | - |
dc.language | eng | - |
dc.publisher | Nature Research | - |
dc.relation.ispartof | Scientific Reports | - |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.title | Polymorphism analysis of pfmdr1 gene in Plasmodium falciparum isolates 11 years post-adoption of artemisinin-based combination therapy in Saudi Arabia | - |
dc.type | Article | - |
dc.identifier.doi | 10.1038/s41598-021-04450-x | - |
dc.identifier.scopus | eid_2-s2.0-85122808317 | - |
dc.identifier.volume | 12 | - |
dc.identifier.issue | 1 | - |
dc.identifier.eissn | 2045-2322 | - |
dc.identifier.isi | WOS:000783767400034 | - |
dc.identifier.issnl | 2045-2322 | - |