File Download
There are no files associated with this item.
Links for fulltext
(May Require Subscription)
- Publisher Website: 10.1016/j.jsb.2023.108000
- Scopus: eid_2-s2.0-85165540730
- Find via
Supplementary
-
Citations:
- Scopus: 0
- Appears in Collections:
Article: Towards in situ high-resolution imaging of viruses and macromolecular complexes using cryo-electron tomography
Title | Towards in situ high-resolution imaging of viruses and macromolecular complexes using cryo-electron tomography |
---|---|
Authors | |
Keywords | A priori information Cryo-electron tomography Subtomogram averaging Virus assembly |
Issue Date | 1-Sep-2023 |
Publisher | Elsevier |
Citation | Journal of Structural Biology, 2023, v. 215, n. 3 How to Cite? |
Abstract | Cryo-electron tomography and subtomogram averaging are rising and fast-evolving imaging techniques to study biological events, providing structural information at an unprecedented resolution while preserving spatial correlation in their native contexts. The latest technology and methodology development ranging from sample preparation to data collection and data processing, has enabled significant advancement in its applications to various biological systems. This review provides an overview of the current technology development enabling high-resolution structural study in situ, highlighting the use of a priori information of biological samples to assess the quality of subtomogram averaging pipeline. We exemplify the applications of this technique to understanding viruses and principles of macromolecule assembly using different biological systems, ranging from in vitro to in situ samples, which provide structural information at different resolutions and contexts. |
Persistent Identifier | http://hdl.handle.net/10722/338086 |
ISSN | 2021 Impact Factor: 3.234 2020 SCImago Journal Rankings: 2.564 |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Huang, Yixin | - |
dc.contributor.author | Zhang, Yu | - |
dc.contributor.author | Ni, Tao | - |
dc.date.accessioned | 2024-03-11T10:26:09Z | - |
dc.date.available | 2024-03-11T10:26:09Z | - |
dc.date.issued | 2023-09-01 | - |
dc.identifier.citation | Journal of Structural Biology, 2023, v. 215, n. 3 | - |
dc.identifier.issn | 1047-8477 | - |
dc.identifier.uri | http://hdl.handle.net/10722/338086 | - |
dc.description.abstract | <p>Cryo-electron tomography and subtomogram averaging are rising and fast-evolving imaging techniques to study biological events, providing structural information at an unprecedented resolution while preserving spatial correlation in their native contexts. The latest technology and methodology development ranging from sample preparation to data collection and data processing, has enabled significant advancement in its applications to various biological systems. This review provides an overview of the current technology development enabling high-resolution structural study in situ, highlighting the use of a priori information of biological samples to assess the quality of subtomogram averaging pipeline. We exemplify the applications of this technique to understanding viruses and principles of macromolecule assembly using different biological systems, ranging from in vitro to in situ samples, which provide structural information at different resolutions and contexts. <br></p> | - |
dc.language | eng | - |
dc.publisher | Elsevier | - |
dc.relation.ispartof | Journal of Structural Biology | - |
dc.subject | A priori information | - |
dc.subject | Cryo-electron tomography | - |
dc.subject | Subtomogram averaging | - |
dc.subject | Virus assembly | - |
dc.title | Towards in situ high-resolution imaging of viruses and macromolecular complexes using cryo-electron tomography | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.jsb.2023.108000 | - |
dc.identifier.scopus | eid_2-s2.0-85165540730 | - |
dc.identifier.volume | 215 | - |
dc.identifier.issue | 3 | - |
dc.identifier.issnl | 1047-8477 | - |