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- Publisher Website: 10.1038/s41467-023-41211-y
- Scopus: eid_2-s2.0-85170184951
- WOS: WOS:001065300300006
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Article: Intrinsically disordered CsoS2 acts as a general molecular thread for α-carboxysome shell assembly
Title | Intrinsically disordered CsoS2 acts as a general molecular thread for α-carboxysome shell assembly |
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Authors | |
Issue Date | 7-Sep-2023 |
Publisher | Nature Research |
Citation | Nature Communications, 2023, v. 14, n. 1 How to Cite? |
Abstract | Carboxysomes are a paradigm of self-assembling proteinaceous organelles found in nature, offering compartmentalisation of enzymes and pathways to enhance carbon fixation. In α-carboxysomes, the disordered linker protein CsoS2 plays an essential role in carboxysome assembly and Rubisco encapsulation. Its mechanism of action, however, is not fully understood. Here we synthetically engineer α-carboxysome shells using minimal shell components and determine cryoEM structures of these to decipher the principle of shell assembly and encapsulation. The structures reveal that the intrinsically disordered CsoS2 C-terminus is well-structured and acts as a universal "molecular thread" stitching through multiple shell protein interfaces. We further uncover in CsoS2 a highly conserved repetitive key interaction motif, [IV]TG, which is critical to the shell assembly and architecture. Our study provides a general mechanism for the CsoS2-governed carboxysome shell assembly and cargo encapsulation and further advances synthetic engineering of carboxysomes for diverse biotechnological applications. |
Persistent Identifier | http://hdl.handle.net/10722/338087 |
ISSN | 2023 Impact Factor: 14.7 2023 SCImago Journal Rankings: 4.887 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Ni, Tao | - |
dc.contributor.author | Jiang, Qiuyao | - |
dc.contributor.author | Ng, Pei Cing | - |
dc.contributor.author | Shen, Juan | - |
dc.contributor.author | Dou, Hao | - |
dc.contributor.author | Zhu, Yanan | - |
dc.contributor.author | Radecke, Julika | - |
dc.contributor.author | Dykes, Gregory F | - |
dc.contributor.author | Huang, Fang | - |
dc.contributor.author | Liu, Lu-Ning | - |
dc.contributor.author | Zhang, Peijun | - |
dc.date.accessioned | 2024-03-11T10:26:09Z | - |
dc.date.available | 2024-03-11T10:26:09Z | - |
dc.date.issued | 2023-09-07 | - |
dc.identifier.citation | Nature Communications, 2023, v. 14, n. 1 | - |
dc.identifier.issn | 2041-1723 | - |
dc.identifier.uri | http://hdl.handle.net/10722/338087 | - |
dc.description.abstract | <p>Carboxysomes are a paradigm of self-assembling proteinaceous organelles found in nature, offering compartmentalisation of enzymes and pathways to enhance carbon fixation. In α-carboxysomes, the disordered linker protein CsoS2 plays an essential role in carboxysome assembly and Rubisco encapsulation. Its mechanism of action, however, is not fully understood. Here we synthetically engineer α-carboxysome shells using minimal shell components and determine cryoEM structures of these to decipher the principle of shell assembly and encapsulation. The structures reveal that the intrinsically disordered CsoS2 C-terminus is well-structured and acts as a universal "molecular thread" stitching through multiple shell protein interfaces. We further uncover in CsoS2 a highly conserved repetitive key interaction motif, [IV]TG, which is critical to the shell assembly and architecture. Our study provides a general mechanism for the CsoS2-governed carboxysome shell assembly and cargo encapsulation and further advances synthetic engineering of carboxysomes for diverse biotechnological applications. <br></p> | - |
dc.language | eng | - |
dc.publisher | Nature Research | - |
dc.relation.ispartof | Nature Communications | - |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.title | Intrinsically disordered CsoS2 acts as a general molecular thread for α-carboxysome shell assembly | - |
dc.type | Article | - |
dc.identifier.doi | 10.1038/s41467-023-41211-y | - |
dc.identifier.scopus | eid_2-s2.0-85170184951 | - |
dc.identifier.volume | 14 | - |
dc.identifier.issue | 1 | - |
dc.identifier.eissn | 2041-1723 | - |
dc.identifier.isi | WOS:001065300300006 | - |
dc.identifier.issnl | 2041-1723 | - |