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Article: The male pachynema-specific protein MAPS drives phase separation in vitro and regulates sex body formation and chromatin behaviors in vivo

TitleThe male pachynema-specific protein MAPS drives phase separation in vitro and regulates sex body formation and chromatin behaviors in vivo
Authors
Lin, ZexiongLi, DongliangZheng, JiahuanYao, ChenchengLiu, DongtengZhang, HaoFeng, HaiweiChen, ChunxuLi, PengZhang, YuxiangJiang, BinjieHu, ZheZhao, YuShi, FuCao, DandanRodriguez-Wallberg, Kenny ALi, ZhengYeung, William SBChow, Louise TWang, HengbinLiu, Kui
KeywordsCP: Cell biology
CP: Molecular biology
male infertility
MAPS
meiotic prophase I
phase separation
sex body formation
Issue Date23-Jan-2024
PublisherCell Press
Citation
Cell Reports, 2024, v. 43, n. 1, p. 1-17 How to Cite?
DOI: http://dx.doi.org/10.1016/j.celrep.2023.113651
Abstract

Dynamic chromosome remodeling and nuclear compartmentalization take place during mammalian meiotic prophase I. We report here that the crucial roles of male pachynema-specific protein (MAPS) in pachynema progression might be mediated by its liquid-liquid phase separation in vitro and in cellulo. MAPS forms distinguishable liquid phases, and deletion or mutations of its N-terminal amino acids (aa) 2−9 disrupt its secondary structure and charge properties, impeding phase separation. Maps−/− pachytene spermatocytesexhibit defects in nucleus compartmentalization, including defects in forming sex bodies, altered nucleosome composition, and disordered chromatin accessibility. MapsΔ2–9/Δ2–9 male mice expressing MAPS protein lacking aa 2–9 phenocopy Maps−/− mice. Moreover, a frameshift mutation in C3orf62, the human counterpart of Maps, is correlated with nonobstructive azoospermia in a patient exhibiting pachynema arrest in spermatocyte development. Hence, the phase separation property of MAPS seems essential for pachynema progression in mouse and human spermatocytes.


Persistent Identifierhttp://hdl.handle.net/10722/339832
ISSN
2211-1247
2023 Impact Factor: 7.5
2023 SCImago Journal Rankings: 4.279
ISI Accession Number ID
WOS:001154262900001

 

DC FieldValueLanguage
dc.contributor.authorLin, Zexiong-
dc.contributor.authorLi, Dongliang-
dc.contributor.authorZheng, Jiahuan-
dc.contributor.authorYao, Chencheng-
dc.contributor.authorLiu, Dongteng-
dc.contributor.authorZhang, Hao-
dc.contributor.authorFeng, Haiwei-
dc.contributor.authorChen, Chunxu-
dc.contributor.authorLi, Peng-
dc.contributor.authorZhang, Yuxiang-
dc.contributor.authorJiang, Binjie-
dc.contributor.authorHu, Zhe-
dc.contributor.authorZhao, Yu-
dc.contributor.authorShi, Fu-
dc.contributor.authorCao, Dandan-
dc.contributor.authorRodriguez-Wallberg, Kenny A-
dc.contributor.authorLi, Zheng-
dc.contributor.authorYeung, William SB-
dc.contributor.authorChow, Louise T-
dc.contributor.authorWang, Hengbin-
dc.contributor.authorLiu, Kui-
dc.date.accessioned2024-03-11T10:39:37Z-
dc.date.available2024-03-11T10:39:37Z-
dc.date.issued2024-01-23-
dc.identifier.citationCell Reports, 2024, v. 43, n. 1, p. 1-17-
dc.identifier.issn2211-1247-
dc.identifier.urihttp://hdl.handle.net/10722/339832-
dc.description.abstract<p>Dynamic chromosome remodeling and nuclear compartmentalization take place during mammalian meiotic <a href="https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/prophase" title="Learn more about prophase from ScienceDirect's AI-generated Topic Pages">prophase</a> I. We report here that the crucial roles of male pachynema-specific protein (MAPS) in <a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/pachytene" title="Learn more about pachynema from ScienceDirect's AI-generated Topic Pages">pachynema</a> progression might be mediated by its liquid-liquid phase separation <em>in vitro</em> and <em>in cellulo</em>. MAPS forms distinguishable liquid phases, and deletion or mutations of its N-terminal <a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/amino-acids" title="Learn more about amino acids from ScienceDirect's AI-generated Topic Pages">amino acids</a> (aa) 2−9 disrupt its <a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/protein-secondary-structure" title="Learn more about secondary structure from ScienceDirect's AI-generated Topic Pages">secondary structure</a> and charge properties, impeding phase separation. <em>Maps</em><em>−/−</em> pachytene <a href="https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/gametocyte" title="Learn more about spermatocytes from ScienceDirect's AI-generated Topic Pages">spermatocytes</a>exhibit defects in nucleus compartmentalization, including defects in forming sex bodies, altered nucleosome composition, and disordered chromatin accessibility. <em>Maps</em><em>Δ2–9/Δ2–9</em> male mice expressing MAPS protein lacking aa 2–9 <a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/phenocopy" title="Learn more about phenocopy from ScienceDirect's AI-generated Topic Pages">phenocopy</a> <em>Maps</em><em>−/−</em> mice. Moreover, a <a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/frameshift-mutation" title="Learn more about frameshift mutation from ScienceDirect's AI-generated Topic Pages">frameshift mutation</a> in <em>C3orf62</em>, the human counterpart of <em>Maps</em>, is correlated with nonobstructive azoospermia in a patient exhibiting pachynema arrest in spermatocyte development. Hence, the phase separation property of MAPS seems essential for pachynema progression in mouse and human spermatocytes.<br></p>-
dc.languageeng-
dc.publisherCell Press-
dc.relation.ispartofCell Reports-
dc.subjectCP: Cell biology-
dc.subjectCP: Molecular biology-
dc.subjectmale infertility-
dc.subjectMAPS-
dc.subjectmeiotic prophase I-
dc.subjectphase separation-
dc.subjectsex body formation-
dc.titleThe male pachynema-specific protein MAPS drives phase separation in vitro and regulates sex body formation and chromatin behaviors in vivo-
dc.typeArticle-
dc.identifier.doi10.1016/j.celrep.2023.113651-
dc.identifier.scopuseid_2-s2.0-85183405888-
dc.identifier.volume43-
dc.identifier.issue1-
dc.identifier.spage1-
dc.identifier.epage17-
dc.identifier.isiWOS:001154262900001-
dc.identifier.issnl2211-1247-

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