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Article: Content of stress granules reveals a sex difference at the early phase of cold exposure in mice

TitleContent of stress granules reveals a sex difference at the early phase of cold exposure in mice
Authors
Keywordsadaptive thermogenesis
sex dimorphism
stress granules
Issue Date21-Dec-2023
PublisherAmerican Physiological Society
Citation
AJP - Endocrinology and Metabolism, 2024, v. 326, n. 1, p. E29-E37 How to Cite?
Abstract

Adaptive thermogenesis is a vital physiological process for small endotherms. Female animals usually are more sensitive to cold temperature due to anatomical differences. Whether there is a sex difference at a molecular level is unclear. Stress granules (SGs) are dynamic organelles in which untranslated mRNAs reside during cellular stress. We hypothesize that the prompt response of SGs to cold stress can reveal the molecular difference between sexes. By analyzing the content in SGs of brown adipose tissue (BAT) at the early phase of cold stress for both sexes, we found more diverse mRNAs docked in the SGs in male mice and these mRNAs representing an extensive cellular reprogramming including apoptosis process and cold-induced thermogenesis. In female mice, the mRNAs in SGs dominantly were comprised of genes regulating ribonucleoprotein complex biogenesis. Conversely, the proteome in SGs was commonly characterized as structure molecules and RNA processing for both sexes. A spectrum of eukaryotic initiation factors (eIFs) was detected in the SGs of both female and male BAT, while those remained unchanged upon cold stress in male mice, various eIF3 and eIF4G isoforms were found reduced in female mice. Taken together, the unique features in SGs of male BAT reflected a prompt uncoupling protein-1 (UCP1) induction which was absent in female, and female, by contrast, were prepared for long-term transcriptional and translational adaptations.NEW & NOTEWORTHY The proteome analysis reveals that stress granules are the predominant form of cytosolic messenger ribonucleoproteins of brown adipose tissue (BAT) at the early phase of cold exposure in mice for both sexes. The transcriptome of stress granules of BAT unveils a sex difference of molecular response in early phase of cold exposure in mice, and such difference prepares for a prompt response to cold stress in male mice while for long-term adaptation in female mice.


Persistent Identifierhttp://hdl.handle.net/10722/339853
ISSN
2023 Impact Factor: 4.2
2023 SCImago Journal Rankings: 1.479
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorCheung, SWM-
dc.contributor.authorYiu, JHC-
dc.contributor.authorChin, KTC-
dc.contributor.authorCai, JL-
dc.contributor.authorXu, AM-
dc.contributor.authorWong, CM-
dc.contributor.authorWoo, CW -
dc.date.accessioned2024-03-11T10:39:47Z-
dc.date.available2024-03-11T10:39:47Z-
dc.date.issued2023-12-21-
dc.identifier.citationAJP - Endocrinology and Metabolism, 2024, v. 326, n. 1, p. E29-E37-
dc.identifier.issn0193-1849-
dc.identifier.urihttp://hdl.handle.net/10722/339853-
dc.description.abstract<p>Adaptive thermogenesis is a vital physiological process for small endotherms. Female animals usually are more sensitive to cold temperature due to anatomical differences. Whether there is a sex difference at a molecular level is unclear. Stress granules (SGs) are dynamic organelles in which untranslated mRNAs reside during cellular stress. We hypothesize that the prompt response of SGs to cold stress can reveal the molecular difference between sexes. By analyzing the content in SGs of brown adipose tissue (BAT) at the early phase of cold stress for both sexes, we found more diverse mRNAs docked in the SGs in male mice and these mRNAs representing an extensive cellular reprogramming including apoptosis process and cold-induced thermogenesis. In female mice, the mRNAs in SGs dominantly were comprised of genes regulating ribonucleoprotein complex biogenesis. Conversely, the proteome in SGs was commonly characterized as structure molecules and RNA processing for both sexes. A spectrum of eukaryotic initiation factors (eIFs) was detected in the SGs of both female and male BAT, while those remained unchanged upon cold stress in male mice, various eIF3 and eIF4G isoforms were found reduced in female mice. Taken together, the unique features in SGs of male BAT reflected a prompt uncoupling protein-1 (UCP1) induction which was absent in female, and female, by contrast, were prepared for long-term transcriptional and translational adaptations.NEW & NOTEWORTHY The proteome analysis reveals that stress granules are the predominant form of cytosolic messenger ribonucleoproteins of brown adipose tissue (BAT) at the early phase of cold exposure in mice for both sexes. The transcriptome of stress granules of BAT unveils a sex difference of molecular response in early phase of cold exposure in mice, and such difference prepares for a prompt response to cold stress in male mice while for long-term adaptation in female mice.</p>-
dc.languageeng-
dc.publisherAmerican Physiological Society-
dc.relation.ispartofAJP - Endocrinology and Metabolism-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectadaptive thermogenesis-
dc.subjectsex dimorphism-
dc.subjectstress granules-
dc.titleContent of stress granules reveals a sex difference at the early phase of cold exposure in mice-
dc.typeArticle-
dc.identifier.doi10.1152/ajpendo.00317.2023-
dc.identifier.pmid37991452-
dc.identifier.scopuseid_2-s2.0-85181179091-
dc.identifier.volume326-
dc.identifier.issue1-
dc.identifier.spageE29-
dc.identifier.epageE37-
dc.identifier.eissn1522-1555-
dc.identifier.isiWOS:001143708400003-
dc.identifier.issnl0193-1849-

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