File Download
There are no files associated with this item.
Links for fulltext
(May Require Subscription)
- Publisher Website: 10.3791/64677
- Scopus: eid_2-s2.0-85149509347
- WOS: WOS:000992754400001
- Find via
Supplementary
- Citations:
- Appears in Collections:
Article: Facile Preparation and Photoactivation of Prodrug-Dye Nanoassemblies
Title | Facile Preparation and Photoactivation of Prodrug-Dye Nanoassemblies |
---|---|
Authors | |
Issue Date | 17-Feb-2023 |
Publisher | MyJove Corporation |
Citation | Journal of Visualized Experiments, 2023, v. 2023, n. 192 How to Cite? |
Abstract | Self-assembly is a simple yet reliable method for constructing nanoscale drug delivery systems. Photoactivatable prodrugs enable controllable drug release from nanocarriers at target sites modulated by light irradiation. In this protocol, a facile method for fabricating photoactivatable prodrug-dye nanoparticles via molecular self-assembly is presented. The procedures for prodrug synthesis, nanoparticle fabrication, physical characterization of the nanoassembly, photocleavage demonstration, and in vitro cytotoxicity verification are described in detail. A photocleavable boron-dipyrromethene-chlorambucil (BC) prodrug was first synthesized. BC and a near-infrared dye, IR-783, at an optimized ratio, could self-assemble into nanoparticles (IR783/BC NPs). The synthesized nanoparticles had an average size of 87.22 nm and a surface charge of -29.8 mV. The nanoparticles disassembled upon light irradiation, which could be observed by transmission electronic microscopy. The photocleavage of BC was completed within 10 min, with a 22% recovery efficiency for chlorambucil. The nanoparticles displayed enhanced cytotoxicity under light irradiation at 530 nm compared with the non-irradiated nanoparticles and irradiated free BC prodrug. This protocol provides a reference for the construction and evaluation of photoresponsive drug delivery systems. |
Persistent Identifier | http://hdl.handle.net/10722/340562 |
ISSN | 2023 Impact Factor: 1.2 2023 SCImago Journal Rankings: 0.449 |
ISI Accession Number ID |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Zhang, Yichi | - |
dc.contributor.author | Long, Kaiqi | - |
dc.contributor.author | Wang, Weiping | - |
dc.date.accessioned | 2024-03-11T10:45:31Z | - |
dc.date.available | 2024-03-11T10:45:31Z | - |
dc.date.issued | 2023-02-17 | - |
dc.identifier.citation | Journal of Visualized Experiments, 2023, v. 2023, n. 192 | - |
dc.identifier.issn | 1940-087X | - |
dc.identifier.uri | http://hdl.handle.net/10722/340562 | - |
dc.description.abstract | <p>Self-assembly is a simple yet reliable method for constructing nanoscale drug delivery systems. Photoactivatable prodrugs enable controllable drug release from nanocarriers at target sites modulated by light irradiation. In this protocol, a facile method for fabricating photoactivatable prodrug-dye nanoparticles <em>via </em>molecular self-assembly is presented. The procedures for prodrug synthesis, nanoparticle fabrication, physical characterization of the nanoassembly, photocleavage demonstration, and <em>in vitro</em> cytotoxicity verification are described in detail. A photocleavable boron-dipyrromethene-chlorambucil (BC) prodrug was first synthesized. BC and a near-infrared dye, IR-783, at an optimized ratio, could self-assemble into nanoparticles (IR783/BC NPs). The synthesized nanoparticles had an average size of 87.22 nm and a surface charge of -29.8 mV. The nanoparticles disassembled upon light irradiation, which could be observed by transmission electronic microscopy. The photocleavage of BC was completed within 10 min, with a 22% recovery efficiency for chlorambucil. The nanoparticles displayed enhanced cytotoxicity under light irradiation at 530 nm compared with the non-irradiated nanoparticles and irradiated free BC prodrug. This protocol provides a reference for the construction and evaluation of photoresponsive drug delivery systems.<br></p> | - |
dc.language | eng | - |
dc.publisher | MyJove Corporation | - |
dc.relation.ispartof | Journal of Visualized Experiments | - |
dc.title | Facile Preparation and Photoactivation of Prodrug-Dye Nanoassemblies | - |
dc.type | Article | - |
dc.identifier.doi | 10.3791/64677 | - |
dc.identifier.scopus | eid_2-s2.0-85149509347 | - |
dc.identifier.volume | 2023 | - |
dc.identifier.issue | 192 | - |
dc.identifier.eissn | 1940-087X | - |
dc.identifier.isi | WOS:000992754400001 | - |
dc.identifier.issnl | 1940-087X | - |