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Article: The lipoprotein lipase that is shuttled into capillaries by GPIHBP1 enters the glycocalyx where it mediates lipoprotein processing

TitleThe lipoprotein lipase that is shuttled into capillaries by GPIHBP1 enters the glycocalyx where it mediates lipoprotein processing
Authors
Issue Date23-Oct-2023
PublisherNational Academy of Sciences
Citation
Proceedings of the National Academy of Sciences, 2023, v. 120, n. 44 How to Cite?
Abstract

Lipoprotein lipase (LPL), the enzyme that carries out the lipolytic processing of triglyceride-rich lipoproteins (TRLs), is synthesized by adipocytes and myocytes and secreted into the interstitial spaces. The LPL is then bound by GPIHBP1, a GPI-anchored protein of endothelial cells (ECs), and transported across ECs to the capillary lumen. The assumption has been that the LPL that is moved into capillaries remains attached to GPIHBP1 and that GPIHBP1 serves as a platform for TRL processing. In the current studies, we examined the validity of that assumption. We found that an LPL-specific monoclonal antibody (mAb), 88B8, which lacks the ability to detect GPIHBP1-bound LPL, binds avidly to LPL within capillaries. We further demonstrated, by confocal microscopy, immunogold electron microscopy, and nanoscale secondary ion mass spectrometry analyses, that the LPL detected by mAb 88B8 is located within the EC glycocalyx, distant from the GPIHBP1 on the EC plasma membrane. The LPL within the glycocalyx mediates the margination of TRLs along capillaries and is active in TRL processing, resulting in the delivery of lipoprotein-derived lipids to immediately adjacent parenchymal cells. Thus, the LPL that GPIHBP1 transports into capillaries can detach and move into the EC glycocalyx, where it functions in the intravascular processing of TRLs.


Persistent Identifierhttp://hdl.handle.net/10722/340651
ISSN
2023 Impact Factor: 9.4
2023 SCImago Journal Rankings: 3.737
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorSong, Wenxin-
dc.contributor.authorBeigneux, Anne P-
dc.contributor.authorWeston, Thomas A-
dc.contributor.authorChen, Kai-
dc.contributor.authorYang, Ye-
dc.contributor.authorNguyen, le Phuong-
dc.contributor.authorGuagliardo, Paul-
dc.contributor.authorJung, Hyesoo-
dc.contributor.authorTran, Anh P-
dc.contributor.authorTu, Yiping-
dc.contributor.authorTran, Caitlyn-
dc.contributor.authorBirrane, Gabriel-
dc.contributor.authorMiyashita, Kazuya-
dc.contributor.authorNakajima, Katsuyuki-
dc.contributor.authorMurakami, Masami-
dc.contributor.authorTontonoz, Peter-
dc.contributor.authorJiang, Haibo-
dc.contributor.authorPloug, Michael-
dc.contributor.authorFong, Loren G-
dc.contributor.authorYoung, Stephen G -
dc.date.accessioned2024-03-11T10:46:09Z-
dc.date.available2024-03-11T10:46:09Z-
dc.date.issued2023-10-23-
dc.identifier.citationProceedings of the National Academy of Sciences, 2023, v. 120, n. 44-
dc.identifier.issn0027-8424-
dc.identifier.urihttp://hdl.handle.net/10722/340651-
dc.description.abstract<p>Lipoprotein lipase (LPL), the enzyme that carries out the lipolytic processing of triglyceride-rich lipoproteins (TRLs), is synthesized by adipocytes and myocytes and secreted into the interstitial spaces. The LPL is then bound by GPIHBP1, a GPI-anchored protein of endothelial cells (ECs), and transported across ECs to the capillary lumen. The assumption has been that the LPL that is moved into capillaries remains attached to GPIHBP1 and that GPIHBP1 serves as a platform for TRL processing. In the current studies, we examined the validity of that assumption. We found that an LPL-specific monoclonal antibody (mAb), 88B8, which lacks the ability to detect GPIHBP1-bound LPL, binds avidly to LPL within capillaries. We further demonstrated, by confocal microscopy, immunogold electron microscopy, and nanoscale secondary ion mass spectrometry analyses, that the LPL detected by mAb 88B8 is located within the EC glycocalyx, distant from the GPIHBP1 on the EC plasma membrane. The LPL within the glycocalyx mediates the margination of TRLs along capillaries and is active in TRL processing, resulting in the delivery of lipoprotein-derived lipids to immediately adjacent parenchymal cells. Thus, the LPL that GPIHBP1 transports into capillaries can detach and move into the EC glycocalyx, where it functions in the intravascular processing of TRLs.<br></p>-
dc.languageeng-
dc.publisherNational Academy of Sciences-
dc.relation.ispartofProceedings of the National Academy of Sciences-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.titleThe lipoprotein lipase that is shuttled into capillaries by GPIHBP1 enters the glycocalyx where it mediates lipoprotein processing-
dc.typeArticle-
dc.identifier.doi10.1073/pnas.2313825120-
dc.identifier.scopuseid_2-s2.0-85175661671-
dc.identifier.volume120-
dc.identifier.issue44-
dc.identifier.eissn1091-6490-
dc.identifier.isiWOS:001124085100003-
dc.identifier.issnl0027-8424-

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