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Article: High-Throughput Generation, Manipulation, and Degradation of Magnetic Nanoparticle-Laden Alginate Core-Shell Beads for Single Bacteria Culturing Analysis

TitleHigh-Throughput Generation, Manipulation, and Degradation of Magnetic Nanoparticle-Laden Alginate Core-Shell Beads for Single Bacteria Culturing Analysis
Authors
Keywordsbacteria culturing
bacteria screening
biomechatronics
magnetic sorting
Microfluidics
Issue Date1-Jul-2023
PublisherInstitute of Electrical and Electronics Engineers
Citation
IEEE Transactions on NanoBioscience, 2023, v. 22, n. 3, p. 487-497 How to Cite?
Abstract

Microbes could be found almost everywhere around us and have significant impacts on our human society. The treatment of microorganisms has long been seen as a complex problem. Till now, most of the genetic and phenotypic information regarding rare species is buried in the bulk microbial colony due to a lack of efficient tools to screen live bacteria. Droplet microfluidics offers a powerful approach to address this problem. However, the interactions among bacteria and their living environment are entirely restricted by the water/oil interfaces in conventional water/oil single emulsion-based microfluidic systems. Here, we demonstrate an oil-mediated all-aqueous microfluidic workflow that can overcome this drawback. In contrast to the previous works, our all-aqueous culturing environment allows cell-cell and cell-environment interactions, thus facilitating the growth of bacteria. Fe3O4 magnetic nanoparticles added into the alginate beads enables on-chip manipulation of the microcapsules. The core-shell structure separately encapsulates bacteria and magnetic particles in the core and shell to avoid contamination. We demonstrate the feasibility of this approach by single bacterium culturing in droplet-templated alginate beads. Finally, a new approach is proposed to degrade the alginate beads for post-treatment. This novel microfluidic workflow can create new opportunities for microbial applications, such as bacteria culturing and screening.


Persistent Identifierhttp://hdl.handle.net/10722/340939
ISSN
2023 Impact Factor: 3.7
2023 SCImago Journal Rankings: 0.659
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorYuan, S-
dc.contributor.authorZhang, Y-
dc.contributor.authorNan, L-
dc.contributor.authorLai, PT-
dc.contributor.authorZhang, T-
dc.contributor.authorPong, PWT-
dc.contributor.authorShum, HC-
dc.date.accessioned2024-03-11T10:48:26Z-
dc.date.available2024-03-11T10:48:26Z-
dc.date.issued2023-07-01-
dc.identifier.citationIEEE Transactions on NanoBioscience, 2023, v. 22, n. 3, p. 487-497-
dc.identifier.issn1536-1241-
dc.identifier.urihttp://hdl.handle.net/10722/340939-
dc.description.abstract<p>Microbes could be found almost everywhere around us and have significant impacts on our human society. The treatment of microorganisms has long been seen as a complex problem. Till now, most of the genetic and phenotypic information regarding rare species is buried in the bulk microbial colony due to a lack of efficient tools to screen live bacteria. Droplet microfluidics offers a powerful approach to address this problem. However, the interactions among bacteria and their living environment are entirely restricted by the water/oil interfaces in conventional water/oil single emulsion-based microfluidic systems. Here, we demonstrate an oil-mediated all-aqueous microfluidic workflow that can overcome this drawback. In contrast to the previous works, our all-aqueous culturing environment allows cell-cell and cell-environment interactions, thus facilitating the growth of bacteria. Fe3O4 magnetic nanoparticles added into the alginate beads enables on-chip manipulation of the microcapsules. The core-shell structure separately encapsulates bacteria and magnetic particles in the core and shell to avoid contamination. We demonstrate the feasibility of this approach by single bacterium culturing in droplet-templated alginate beads. Finally, a new approach is proposed to degrade the alginate beads for post-treatment. This novel microfluidic workflow can create new opportunities for microbial applications, such as bacteria culturing and screening.</p>-
dc.languageeng-
dc.publisherInstitute of Electrical and Electronics Engineers-
dc.relation.ispartofIEEE Transactions on NanoBioscience-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectbacteria culturing-
dc.subjectbacteria screening-
dc.subjectbiomechatronics-
dc.subjectmagnetic sorting-
dc.subjectMicrofluidics-
dc.titleHigh-Throughput Generation, Manipulation, and Degradation of Magnetic Nanoparticle-Laden Alginate Core-Shell Beads for Single Bacteria Culturing Analysis-
dc.typeArticle-
dc.identifier.doi10.1109/TNB.2022.3205057-
dc.identifier.scopuseid_2-s2.0-85137878551-
dc.identifier.volume22-
dc.identifier.issue3-
dc.identifier.spage487-
dc.identifier.epage497-
dc.identifier.eissn1558-2639-
dc.identifier.isiWOS:001023344700004-
dc.identifier.issnl1536-1241-

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