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Article: Ultrasensitive sensors reveal the spatiotemporal landscape of lactate metabolism in physiology and disease

TitleUltrasensitive sensors reveal the spatiotemporal landscape of lactate metabolism in physiology and disease
Authors
Keywordshighly responsive lactate sensors
lactate metabolism
point-of-care clinical screening
real-time monitoring
subcellular lactate landscape
Issue Date2023
Citation
Cell Metabolism, 2023, v. 35, n. 1, p. 200-211.e9 How to Cite?
AbstractDespite its central importance in cellular metabolism, many details remain to be determined regarding subcellular lactate metabolism and its regulation in physiology and disease, as there is sensitive spatiotemporal resolution of lactate distribution, and dynamics remains a technical challenge. Here, we develop and characterize an ultrasensitive, highly responsive, ratiometric lactate sensor, named FiLa, enabling the monitoring of subtle lactate fluctuations in living cells and animals. Utilizing FiLa, we demonstrate that lactate is highly enriched in mammalian mitochondria and compile an atlas of subcellular lactate metabolism that reveals lactate as a key hub sensing various metabolic activities. In addition, FiLa sensors also enable direct imaging of elevated lactate levels in diabetic mice and facilitate the establishment of a simple, rapid, and sensitive lactate assay for point-of-care clinical screening. Thus, FiLa sensors provide powerful, broadly applicable tools for defining the spatiotemporal landscape of lactate metabolism in health and disease.
Persistent Identifierhttp://hdl.handle.net/10722/342259
ISSN
2023 Impact Factor: 27.7
2023 SCImago Journal Rankings: 11.406
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLi, Xie-
dc.contributor.authorZhang, Yinan-
dc.contributor.authorXu, Lingyan-
dc.contributor.authorWang, Aoxue-
dc.contributor.authorZou, Yejun-
dc.contributor.authorLi, Ting-
dc.contributor.authorHuang, Li-
dc.contributor.authorChen, Weicai-
dc.contributor.authorLiu, Shuning-
dc.contributor.authorJiang, Kun-
dc.contributor.authorZhang, Xiuze-
dc.contributor.authorWang, Dongmei-
dc.contributor.authorZhang, Lijuan-
dc.contributor.authorZhang, Zhuo-
dc.contributor.authorZhang, Zeyi-
dc.contributor.authorChen, Xianjun-
dc.contributor.authorJia, Wei-
dc.contributor.authorZhao, Aihua-
dc.contributor.authorYan, Xinfeng-
dc.contributor.authorZhou, Haimeng-
dc.contributor.authorZhu, Linyong-
dc.contributor.authorMa, Xinran-
dc.contributor.authorJu, Zhenyu-
dc.contributor.authorJia, Weiping-
dc.contributor.authorWang, Congrong-
dc.contributor.authorLoscalzo, Joseph-
dc.contributor.authorYang, Yi-
dc.contributor.authorZhao, Yuzheng-
dc.date.accessioned2024-04-17T07:02:31Z-
dc.date.available2024-04-17T07:02:31Z-
dc.date.issued2023-
dc.identifier.citationCell Metabolism, 2023, v. 35, n. 1, p. 200-211.e9-
dc.identifier.issn1550-4131-
dc.identifier.urihttp://hdl.handle.net/10722/342259-
dc.description.abstractDespite its central importance in cellular metabolism, many details remain to be determined regarding subcellular lactate metabolism and its regulation in physiology and disease, as there is sensitive spatiotemporal resolution of lactate distribution, and dynamics remains a technical challenge. Here, we develop and characterize an ultrasensitive, highly responsive, ratiometric lactate sensor, named FiLa, enabling the monitoring of subtle lactate fluctuations in living cells and animals. Utilizing FiLa, we demonstrate that lactate is highly enriched in mammalian mitochondria and compile an atlas of subcellular lactate metabolism that reveals lactate as a key hub sensing various metabolic activities. In addition, FiLa sensors also enable direct imaging of elevated lactate levels in diabetic mice and facilitate the establishment of a simple, rapid, and sensitive lactate assay for point-of-care clinical screening. Thus, FiLa sensors provide powerful, broadly applicable tools for defining the spatiotemporal landscape of lactate metabolism in health and disease.-
dc.languageeng-
dc.relation.ispartofCell Metabolism-
dc.subjecthighly responsive lactate sensors-
dc.subjectlactate metabolism-
dc.subjectpoint-of-care clinical screening-
dc.subjectreal-time monitoring-
dc.subjectsubcellular lactate landscape-
dc.titleUltrasensitive sensors reveal the spatiotemporal landscape of lactate metabolism in physiology and disease-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.cmet.2022.10.002-
dc.identifier.pmid36309010-
dc.identifier.scopuseid_2-s2.0-85144921286-
dc.identifier.volume35-
dc.identifier.issue1-
dc.identifier.spage200-
dc.identifier.epage211.e9-
dc.identifier.eissn1932-7420-
dc.identifier.isiWOS:000920390700001-

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