Effect of Jinfu Kang to experimental precancerous colon lesions and urinary metabolites in rat

Abstract

Objective: To profile urinary metabolite variations from 1, 2-dimethylhydrazine (DMH)-induced precancerous colon rats, Jinfu Kang treated rats and healthy controls. Method: We used ethyl chloroformate derivatization and gas chromatographymass spectrometry (GC-MS) based metabonomic method to analyze rat urines. Result: The time-dependent variations of metabolite profile showed a progressive deviation of the metabolism in die model group from the initial pattern over time and a systemic recovery of the metabolism in the treatment group, which is consistent with the histological results. The in-depth analysis indicated that the disorder of tricarboxylic acid cycle (TCA), tryptophan metabolism, polyamine metabolism and gut flora structure were associated with DMH intervention. Conclusion: Metabolic study revealed that Jinfu Kang can effectively reverse metabolic departures in DMH-induced precancerous colon rat, which is consistent with pathological results.