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Article: Analysis of transcriptome and metabolome profiles alterations in fatty liver induced by high-fat diet in rat

TitleAnalysis of transcriptome and metabolome profiles alterations in fatty liver induced by high-fat diet in rat
Authors
Issue Date2010
Citation
Metabolism: Clinical and Experimental, 2010, v. 59, n. 4, p. 554-560 How to Cite?
AbstractExcessive energy intake greatly contributes to the development of nonalcoholic fatty liver disease (NAFLD) in modern society. To better understand the comprehensive mechanisms of NAFLD development, we investigated the metabolic alterations of rats with NAFLD induced by high-fat diet (HFD). Male Wistar rats were fed a HFD or standard chow for control. After 16 weeks, rat serum was collected for biochemical measurement. The rats' livers were resected and subjected to histology inspection and gene expression analysis with complementary DNA microarray and metabolic analysis with gas chromatography-mass spectroscopy. In HFD rats, the serum cholesterol, triglycerides, glucose, and insulin contents were increased; and the total cholesterol and triglycerides in the livers were also significantly increased. Complementary DNA microarray analysis revealed that 130 genes were regulated by HFD. Together with real-time reverse transcriptase polymerase chain reaction, lipid metabolism regulatory members like sterol regulatory element binding factor 1 and stearoyl-coenzyme A desaturase 1 had up-regulation, whereas others like peroxisome proliferator-activated receptor, carnitine palmitoyltransferase 1, and 3-hydroxy-3-methylglutaryl-coenzyme A reductase had repressed expression, in HFD rat livers. Metabolomic analysis showed that tetradecanoic acid, hexadecanoic acid, and oleic acid had elevation and arachidonic acid and eicosapentaenoic acid had decreased content in HFD rat livers. Amino acids including glycine, alanine, aspartic acid, glutamic acid, and proline contents were decreased. The integrative results from transcriptomic and metabolomic studies revealed that, in HFD rat livers, fatty acid utilization through β-oxidation was inhibited and lipogenesis was enhanced. These observations facilitated our understanding of the pathways involved in the development of NAFLD induced by HFD. © 2010 Elsevier Inc.
Persistent Identifierhttp://hdl.handle.net/10722/342367
ISSN
2023 Impact Factor: 10.8
2023 SCImago Journal Rankings: 2.792
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorXie, Zuoquan-
dc.contributor.authorLi, Houkai-
dc.contributor.authorWang, Ke-
dc.contributor.authorLin, Jingchao-
dc.contributor.authorWang, Qi-
dc.contributor.authorZhao, Guoping-
dc.contributor.authorJia, Wei-
dc.contributor.authorZhang, Qinghua-
dc.date.accessioned2024-04-17T07:03:19Z-
dc.date.available2024-04-17T07:03:19Z-
dc.date.issued2010-
dc.identifier.citationMetabolism: Clinical and Experimental, 2010, v. 59, n. 4, p. 554-560-
dc.identifier.issn0026-0495-
dc.identifier.urihttp://hdl.handle.net/10722/342367-
dc.description.abstractExcessive energy intake greatly contributes to the development of nonalcoholic fatty liver disease (NAFLD) in modern society. To better understand the comprehensive mechanisms of NAFLD development, we investigated the metabolic alterations of rats with NAFLD induced by high-fat diet (HFD). Male Wistar rats were fed a HFD or standard chow for control. After 16 weeks, rat serum was collected for biochemical measurement. The rats' livers were resected and subjected to histology inspection and gene expression analysis with complementary DNA microarray and metabolic analysis with gas chromatography-mass spectroscopy. In HFD rats, the serum cholesterol, triglycerides, glucose, and insulin contents were increased; and the total cholesterol and triglycerides in the livers were also significantly increased. Complementary DNA microarray analysis revealed that 130 genes were regulated by HFD. Together with real-time reverse transcriptase polymerase chain reaction, lipid metabolism regulatory members like sterol regulatory element binding factor 1 and stearoyl-coenzyme A desaturase 1 had up-regulation, whereas others like peroxisome proliferator-activated receptor, carnitine palmitoyltransferase 1, and 3-hydroxy-3-methylglutaryl-coenzyme A reductase had repressed expression, in HFD rat livers. Metabolomic analysis showed that tetradecanoic acid, hexadecanoic acid, and oleic acid had elevation and arachidonic acid and eicosapentaenoic acid had decreased content in HFD rat livers. Amino acids including glycine, alanine, aspartic acid, glutamic acid, and proline contents were decreased. The integrative results from transcriptomic and metabolomic studies revealed that, in HFD rat livers, fatty acid utilization through β-oxidation was inhibited and lipogenesis was enhanced. These observations facilitated our understanding of the pathways involved in the development of NAFLD induced by HFD. © 2010 Elsevier Inc.-
dc.languageeng-
dc.relation.ispartofMetabolism: Clinical and Experimental-
dc.titleAnalysis of transcriptome and metabolome profiles alterations in fatty liver induced by high-fat diet in rat-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.metabol.2009.08.022-
dc.identifier.pmid19913842-
dc.identifier.scopuseid_2-s2.0-77949444189-
dc.identifier.volume59-
dc.identifier.issue4-
dc.identifier.spage554-
dc.identifier.epage560-
dc.identifier.isiWOS:000276189200015-

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