File Download
There are no files associated with this item.
Links for fulltext
(May Require Subscription)
- Publisher Website: 10.1016/j.jpba.2010.01.046
- Scopus: eid_2-s2.0-77950369068
- PMID: 20185264
- WOS: WOS:000276057300021
- Find via
Supplementary
- Citations:
- Appears in Collections:
Article: An optimized procedure for metabonomic analysis of rat liver tissue using gas chromatography/time-of-flight mass spectrometry
Title | An optimized procedure for metabonomic analysis of rat liver tissue using gas chromatography/time-of-flight mass spectrometry |
---|---|
Authors | |
Keywords | Carbon tetrachloride Gas chromatography/time-of-flight mass spectrometry Liver tissue Metabonomics Multivariate statistics |
Issue Date | 2010 |
Citation | Journal of Pharmaceutical and Biomedical Analysis, 2010, v. 52, n. 4, p. 589-596 How to Cite? |
Abstract | In this paper, we present a tissue metabonomic method with an optimized extraction procedure followed by instrumental analysis with gas chromatography/time-of-flight mass spectrometry (GC/TOFMS) and spectral data analysis with multivariate statistics. Metabolite extractions were carried out using three solvents: chloroform, methanol, and water, with design of experiment (DOE) theory and multivariate statistical analysis. A two-step metabolite extraction procedure was optimized using a mixed solvent of chloroform-methanol-water (1:2:1, v/v/v) and then followed by methanol alone. This approach was subsequently validated using standard compounds and liver tissues. Calibration curves were obtained in the range of 0.50-125.0μg/mL for standards and 0.02-0.25 g/mL acceptable for liver tissue samples. For most of the metabolites investigated, relative standard deviations (RSD) were below 10% within a day (reproducibility) and below 15% within a week (stability). Rat liver tissues of carbon tetrachloride-induced acute liver injury models (n=. 10) and healthy control rats (n=. 10) were analyzed which demonstrated the applicability of the developed procedure for the tissue metabonomic study. © 2010 Elsevier B.V. |
Persistent Identifier | http://hdl.handle.net/10722/342369 |
ISSN | 2023 Impact Factor: 3.1 2023 SCImago Journal Rankings: 0.584 |
ISI Accession Number ID |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Pan, Li | - |
dc.contributor.author | Qiu, Yunping | - |
dc.contributor.author | Chen, Tianlu | - |
dc.contributor.author | Lin, Jinchao | - |
dc.contributor.author | Chi, Yi | - |
dc.contributor.author | Su, Mingming | - |
dc.contributor.author | Zhao, Aihua | - |
dc.contributor.author | Jia, Wei | - |
dc.date.accessioned | 2024-04-17T07:03:20Z | - |
dc.date.available | 2024-04-17T07:03:20Z | - |
dc.date.issued | 2010 | - |
dc.identifier.citation | Journal of Pharmaceutical and Biomedical Analysis, 2010, v. 52, n. 4, p. 589-596 | - |
dc.identifier.issn | 0731-7085 | - |
dc.identifier.uri | http://hdl.handle.net/10722/342369 | - |
dc.description.abstract | In this paper, we present a tissue metabonomic method with an optimized extraction procedure followed by instrumental analysis with gas chromatography/time-of-flight mass spectrometry (GC/TOFMS) and spectral data analysis with multivariate statistics. Metabolite extractions were carried out using three solvents: chloroform, methanol, and water, with design of experiment (DOE) theory and multivariate statistical analysis. A two-step metabolite extraction procedure was optimized using a mixed solvent of chloroform-methanol-water (1:2:1, v/v/v) and then followed by methanol alone. This approach was subsequently validated using standard compounds and liver tissues. Calibration curves were obtained in the range of 0.50-125.0μg/mL for standards and 0.02-0.25 g/mL acceptable for liver tissue samples. For most of the metabolites investigated, relative standard deviations (RSD) were below 10% within a day (reproducibility) and below 15% within a week (stability). Rat liver tissues of carbon tetrachloride-induced acute liver injury models (n=. 10) and healthy control rats (n=. 10) were analyzed which demonstrated the applicability of the developed procedure for the tissue metabonomic study. © 2010 Elsevier B.V. | - |
dc.language | eng | - |
dc.relation.ispartof | Journal of Pharmaceutical and Biomedical Analysis | - |
dc.subject | Carbon tetrachloride | - |
dc.subject | Gas chromatography/time-of-flight mass spectrometry | - |
dc.subject | Liver tissue | - |
dc.subject | Metabonomics | - |
dc.subject | Multivariate statistics | - |
dc.title | An optimized procedure for metabonomic analysis of rat liver tissue using gas chromatography/time-of-flight mass spectrometry | - |
dc.type | Article | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1016/j.jpba.2010.01.046 | - |
dc.identifier.pmid | 20185264 | - |
dc.identifier.scopus | eid_2-s2.0-77950369068 | - |
dc.identifier.volume | 52 | - |
dc.identifier.issue | 4 | - |
dc.identifier.spage | 589 | - |
dc.identifier.epage | 596 | - |
dc.identifier.isi | WOS:000276057300021 | - |