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Article: Metabonomics reveals metabolite changes in biliary atresia infants

TitleMetabonomics reveals metabolite changes in biliary atresia infants
Authors
Keywordsamino acid
biliary atresia
metabonomics
neonatal hepatitis syndrome
Issue Date2015
Citation
Journal of Proteome Research, 2015, v. 14, n. 6, p. 2569-2574 How to Cite?
AbstractBiliary atresia (BA) is a rare neonatal cholestatic disorder caused by obstruction of extra- and intra-hepatic bile ducts. If untreated, progressive liver cirrhosis will lead to death within 2 years. Early diagnosis and operation improve the outcome significantly. Infants with neonatal hepatitis syndrome (NHS) present similar symptoms, confounding the early diagnosis of BA. The lack of noninvasive diagnostic methods to differentiate BA from NHS greatly delays the surgery of BA infants, thus deteriorating the outcome. Here we performed a metabolomics study in plasma of BA, NHS, and healthy infants using gas chromatography-time-of-flight mass spectrometry. Scores plots of orthogonal partial least-squares discriminant analysis clearly separated BA from NHS and healthy infants. Eighteen metabolites were found to be differentially expressed between BA and NHS, among which seven (l-glutamic acid, l-ornithine, l-isoleucine, l-lysine, l-valine, l-tryptophan, and l-serine) were amino acids. The altered amino acids were quantitatively verified using ultraperformance liquid chromatography-tandem mass spectrometry. Ingenuity pathway analysis revealed the network of "Cellular Function and Maintenance, Hepatic System Development and Function, Neurological Disease" was altered most significantly. This study suggests that plasma metabolic profiling has great potential in differentiating BA from NHS, and amino acid metabolism is significantly different between the two diseases.
Persistent Identifierhttp://hdl.handle.net/10722/342490
ISSN
2023 Impact Factor: 3.8
2023 SCImago Journal Rankings: 1.299
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorZhou, Kejun-
dc.contributor.authorXie, Guoxiang-
dc.contributor.authorWang, Jun-
dc.contributor.authorZhao, Aihua-
dc.contributor.authorLiu, Jiajian-
dc.contributor.authorSu, Mingming-
dc.contributor.authorNi, Yan-
dc.contributor.authorZhou, Ying-
dc.contributor.authorPan, Weihua-
dc.contributor.authorChe, Yanran-
dc.contributor.authorZhang, Ting-
dc.contributor.authorXiao, Yongtao-
dc.contributor.authorWang, Yang-
dc.contributor.authorWen, Jie-
dc.contributor.authorJia, Wei-
dc.contributor.authorCai, Wei-
dc.date.accessioned2024-04-17T07:04:11Z-
dc.date.available2024-04-17T07:04:11Z-
dc.date.issued2015-
dc.identifier.citationJournal of Proteome Research, 2015, v. 14, n. 6, p. 2569-2574-
dc.identifier.issn1535-3893-
dc.identifier.urihttp://hdl.handle.net/10722/342490-
dc.description.abstractBiliary atresia (BA) is a rare neonatal cholestatic disorder caused by obstruction of extra- and intra-hepatic bile ducts. If untreated, progressive liver cirrhosis will lead to death within 2 years. Early diagnosis and operation improve the outcome significantly. Infants with neonatal hepatitis syndrome (NHS) present similar symptoms, confounding the early diagnosis of BA. The lack of noninvasive diagnostic methods to differentiate BA from NHS greatly delays the surgery of BA infants, thus deteriorating the outcome. Here we performed a metabolomics study in plasma of BA, NHS, and healthy infants using gas chromatography-time-of-flight mass spectrometry. Scores plots of orthogonal partial least-squares discriminant analysis clearly separated BA from NHS and healthy infants. Eighteen metabolites were found to be differentially expressed between BA and NHS, among which seven (l-glutamic acid, l-ornithine, l-isoleucine, l-lysine, l-valine, l-tryptophan, and l-serine) were amino acids. The altered amino acids were quantitatively verified using ultraperformance liquid chromatography-tandem mass spectrometry. Ingenuity pathway analysis revealed the network of "Cellular Function and Maintenance, Hepatic System Development and Function, Neurological Disease" was altered most significantly. This study suggests that plasma metabolic profiling has great potential in differentiating BA from NHS, and amino acid metabolism is significantly different between the two diseases.-
dc.languageeng-
dc.relation.ispartofJournal of Proteome Research-
dc.subjectamino acid-
dc.subjectbiliary atresia-
dc.subjectmetabonomics-
dc.subjectneonatal hepatitis syndrome-
dc.titleMetabonomics reveals metabolite changes in biliary atresia infants-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1021/acs.jproteome.5b00125-
dc.identifier.pmid25899098-
dc.identifier.scopuseid_2-s2.0-84930604004-
dc.identifier.volume14-
dc.identifier.issue6-
dc.identifier.spage2569-
dc.identifier.epage2574-
dc.identifier.eissn1535-3907-
dc.identifier.isiWOS:000355962000018-

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