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Article: Catechins protect against acrylamide- and glycidamide-induced cellular toxicity via rescuing cellular apoptosis and DNA damage

TitleCatechins protect against acrylamide- and glycidamide-induced cellular toxicity via rescuing cellular apoptosis and DNA damage
Authors
KeywordsAcrylamide
Cellular apoptosis
DNA damage
Epicatechin
Epigallocatechin gallate
Glycidamide
Issue Date2022
Citation
Food and Chemical Toxicology, 2022, v. 167, article no. 113253 How to Cite?
AbstractAcrylamide (AA) occurs in both various environmental and dietary sources and has raised widespread concern as a probable carcinogen. Glycidamide (GA) is the main genotoxic metabolite through P450 2E1 (CYP2E1). In the present study, we investigated the protective effect of (−)-epigallocatechin gallate (EGCG) and (−)-epicatechin (EC) against AA- and GA-induced hepatotoxicity in HepG2 cells. The results demonstrated that EC and EGCG inhibited AA- and GA-induced cytotoxicity and mitochondria-mediated cellular apoptosis. Moreover, exposure to AA (100 μg/mL) and GA (50 μg/mL) caused cell cycle arrest and DNA damage, while EC and EGCG ranging from 12.5 to 50 μg/mL rescued cell cycle arrest and inhibited DNA damage. Furthermore, EC and EGCG down-regulated pro-apoptotic protein Bax and Caspase 3 after a 24-h treatment in HepG2 cells exposed to AA (100 μg/mL) or GA (50 μg/mL). Also, the intervention with EC or EGCG up-regulated the expression of DNA repair related protein PARP and down-regulated the expression of Cleaved-PARP. Besides, EC exerted better protective effect than EGCG against AA- and GA-induced cytotoxicity in HepG2 cells. Altogether, EC and EGCG were effective in protecting AA- and GA-induced hepatotoxicity via rescuing cellular apoptosis and DNA damage, as well as promoting cell cycle progression in HepG2 cells.
Persistent Identifierhttp://hdl.handle.net/10722/342661
ISSN
2023 Impact Factor: 3.9
2023 SCImago Journal Rankings: 0.780
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorWang, Anli-
dc.contributor.authorChen, Xinyu-
dc.contributor.authorWang, Laizhao-
dc.contributor.authorJia, Wei-
dc.contributor.authorWan, Xuzhi-
dc.contributor.authorJiao, Jingjing-
dc.contributor.authorYao, Weixuan-
dc.contributor.authorZhang, Yu-
dc.date.accessioned2024-04-17T07:05:22Z-
dc.date.available2024-04-17T07:05:22Z-
dc.date.issued2022-
dc.identifier.citationFood and Chemical Toxicology, 2022, v. 167, article no. 113253-
dc.identifier.issn0278-6915-
dc.identifier.urihttp://hdl.handle.net/10722/342661-
dc.description.abstractAcrylamide (AA) occurs in both various environmental and dietary sources and has raised widespread concern as a probable carcinogen. Glycidamide (GA) is the main genotoxic metabolite through P450 2E1 (CYP2E1). In the present study, we investigated the protective effect of (−)-epigallocatechin gallate (EGCG) and (−)-epicatechin (EC) against AA- and GA-induced hepatotoxicity in HepG2 cells. The results demonstrated that EC and EGCG inhibited AA- and GA-induced cytotoxicity and mitochondria-mediated cellular apoptosis. Moreover, exposure to AA (100 μg/mL) and GA (50 μg/mL) caused cell cycle arrest and DNA damage, while EC and EGCG ranging from 12.5 to 50 μg/mL rescued cell cycle arrest and inhibited DNA damage. Furthermore, EC and EGCG down-regulated pro-apoptotic protein Bax and Caspase 3 after a 24-h treatment in HepG2 cells exposed to AA (100 μg/mL) or GA (50 μg/mL). Also, the intervention with EC or EGCG up-regulated the expression of DNA repair related protein PARP and down-regulated the expression of Cleaved-PARP. Besides, EC exerted better protective effect than EGCG against AA- and GA-induced cytotoxicity in HepG2 cells. Altogether, EC and EGCG were effective in protecting AA- and GA-induced hepatotoxicity via rescuing cellular apoptosis and DNA damage, as well as promoting cell cycle progression in HepG2 cells.-
dc.languageeng-
dc.relation.ispartofFood and Chemical Toxicology-
dc.subjectAcrylamide-
dc.subjectCellular apoptosis-
dc.subjectDNA damage-
dc.subjectEpicatechin-
dc.subjectEpigallocatechin gallate-
dc.subjectGlycidamide-
dc.titleCatechins protect against acrylamide- and glycidamide-induced cellular toxicity via rescuing cellular apoptosis and DNA damage-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.fct.2022.113253-
dc.identifier.pmid35738327-
dc.identifier.scopuseid_2-s2.0-85135598602-
dc.identifier.volume167-
dc.identifier.spagearticle no. 113253-
dc.identifier.epagearticle no. 113253-
dc.identifier.eissn1873-6351-
dc.identifier.isiWOS:000828006900002-

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