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- Publisher Website: 10.3390/toxics10100619
- Scopus: eid_2-s2.0-85140598769
- WOS: WOS:000876114800001
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Article: Rapid Simultaneous Determination of Three Synthetic Cannabinoids in Urine and Plasma of Rats Using Ultra-High Performance Liquid Chromatography Tandem Mass Spectrometry
Title | Rapid Simultaneous Determination of Three Synthetic Cannabinoids in Urine and Plasma of Rats Using Ultra-High Performance Liquid Chromatography Tandem Mass Spectrometry |
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Authors | |
Keywords | 4-methylnaphthalen-1-yl-(1-pentylindol-3-yl) methanone LC-MS/MS methyl (1-(5-fluoropentyl)-1H-indazole-3-carbonyl)-L-valinate methyl 2-(1-(4-fluorobenzyl)-1H-indazole-3-carboxamido)-3-methylbutanoate synthetic cannabinoids |
Issue Date | 2022 |
Citation | Toxics, 2022, v. 10, n. 10, article no. 619 How to Cite? |
Abstract | Synthetic cannabinoids, a class of psychoactive compounds, are controlled as new psychoactive substances (NPSs) identified by the early warning system (EWS) of the European Monitoring Centre for Drugs and Drug Addiction (EMCDDA). At present, several new synthetic cannabinoids have appeared in the illegal drug market, including 4-methylnaphthalen-1-yl-(1-pentylindol-3-yl) methanone (JWH-122), methyl (1-(5-fluoropentyl)-1H-indazole-3-carbonyl)-L-valinate (5F-AMB), and methyl 2-(1-(4-fluorobenzyl)-1Hindazole-3-carboxamido)-3-methylbutanoate (AMB-FUBINACA). A convenient, rapid, and highly sensitive analytical method was developed to determine three synthetic cannabinoids in rat plasma and urine. The liquid chromatography tandem mass spectrometry (LC-MS/MS) method was optimized and validated to analyze the three synthetic cannabinoids in rat plasma and urine. The method identified intra-assay precision (1.3–9.0% and 2.8–6.7%), inter-assay precision (3.0–8.6% and 3.9–8.8%), limits of detection (0.003–0.004 ng/mL and 0.00125–0.002 ng/mL) and quantification (0.012–0.016 ng/mL and 0.003–0.005 ng/mL), recovery (95.4–106.8% and 92.0–106.8%) for rat plasma and urine, and the matrix effect (93.4–118.0%) for rat urine, and the correlation coefficients were above 0.99 in the linear range. The established LC-MS/MS method was successfully used to simultaneously detect the JWH-122 and 5F-AMB in rat plasma and JWH-122, 5F-AMB, and AMB-FUBINACA in rat urine. The present study provides methodological support for internal exposure assessment of three synthetic cannabinoids and promotes the quantitative analysis and technical supervision of synthetic cannabinoids. |
Persistent Identifier | http://hdl.handle.net/10722/342667 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Ke, Xing | - |
dc.contributor.author | Tian, Yimei | - |
dc.contributor.author | He, Dandan | - |
dc.contributor.author | Mu, Pengqian | - |
dc.contributor.author | Wan, Xuzhi | - |
dc.contributor.author | Zhang, Lange | - |
dc.contributor.author | Jia, Wei | - |
dc.contributor.author | Wang, Qiao | - |
dc.contributor.author | Fan, Yilei | - |
dc.contributor.author | Zhang, Yu | - |
dc.date.accessioned | 2024-04-17T07:05:24Z | - |
dc.date.available | 2024-04-17T07:05:24Z | - |
dc.date.issued | 2022 | - |
dc.identifier.citation | Toxics, 2022, v. 10, n. 10, article no. 619 | - |
dc.identifier.uri | http://hdl.handle.net/10722/342667 | - |
dc.description.abstract | Synthetic cannabinoids, a class of psychoactive compounds, are controlled as new psychoactive substances (NPSs) identified by the early warning system (EWS) of the European Monitoring Centre for Drugs and Drug Addiction (EMCDDA). At present, several new synthetic cannabinoids have appeared in the illegal drug market, including 4-methylnaphthalen-1-yl-(1-pentylindol-3-yl) methanone (JWH-122), methyl (1-(5-fluoropentyl)-1H-indazole-3-carbonyl)-L-valinate (5F-AMB), and methyl 2-(1-(4-fluorobenzyl)-1Hindazole-3-carboxamido)-3-methylbutanoate (AMB-FUBINACA). A convenient, rapid, and highly sensitive analytical method was developed to determine three synthetic cannabinoids in rat plasma and urine. The liquid chromatography tandem mass spectrometry (LC-MS/MS) method was optimized and validated to analyze the three synthetic cannabinoids in rat plasma and urine. The method identified intra-assay precision (1.3–9.0% and 2.8–6.7%), inter-assay precision (3.0–8.6% and 3.9–8.8%), limits of detection (0.003–0.004 ng/mL and 0.00125–0.002 ng/mL) and quantification (0.012–0.016 ng/mL and 0.003–0.005 ng/mL), recovery (95.4–106.8% and 92.0–106.8%) for rat plasma and urine, and the matrix effect (93.4–118.0%) for rat urine, and the correlation coefficients were above 0.99 in the linear range. The established LC-MS/MS method was successfully used to simultaneously detect the JWH-122 and 5F-AMB in rat plasma and JWH-122, 5F-AMB, and AMB-FUBINACA in rat urine. The present study provides methodological support for internal exposure assessment of three synthetic cannabinoids and promotes the quantitative analysis and technical supervision of synthetic cannabinoids. | - |
dc.language | eng | - |
dc.relation.ispartof | Toxics | - |
dc.subject | 4-methylnaphthalen-1-yl-(1-pentylindol-3-yl) methanone | - |
dc.subject | LC-MS/MS | - |
dc.subject | methyl (1-(5-fluoropentyl)-1H-indazole-3-carbonyl)-L-valinate | - |
dc.subject | methyl 2-(1-(4-fluorobenzyl)-1H-indazole-3-carboxamido)-3-methylbutanoate | - |
dc.subject | synthetic cannabinoids | - |
dc.title | Rapid Simultaneous Determination of Three Synthetic Cannabinoids in Urine and Plasma of Rats Using Ultra-High Performance Liquid Chromatography Tandem Mass Spectrometry | - |
dc.type | Article | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.3390/toxics10100619 | - |
dc.identifier.scopus | eid_2-s2.0-85140598769 | - |
dc.identifier.volume | 10 | - |
dc.identifier.issue | 10 | - |
dc.identifier.spage | article no. 619 | - |
dc.identifier.epage | article no. 619 | - |
dc.identifier.eissn | 2305-6304 | - |
dc.identifier.isi | WOS:000876114800001 | - |