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Article: Distinct urinary metabolic profile of human colorectal cancer

TitleDistinct urinary metabolic profile of human colorectal cancer
Authors
Keywordscolorectal cancer
gas chromatography time-of-flight mass spectrometry
metabolic profiling
metabonomics
ultra performance liquid chromatography quadrupole time-of-flight mass spectrometry
urine
Issue Date2012
Citation
Journal of Proteome Research, 2012, v. 11, n. 2, p. 1354-1363 How to Cite?
AbstractA full spectrum of metabolic aberrations that are directly linked to colorectal cancer (CRC) at early curable stages is critical for developing and deploying molecular diagnostic and therapeutic approaches that will significantly improve patient survival. We have recently reported a urinary metabonomic profiling study on CRC subjects (n = 60) and health controls (n = 63), in which a panel of urinary metabolite markers was identified. Here, we report a second urinary metabonomic study on a larger cohort of CRC (n = 101) and healthy subjects (n = 103), using gas chromatography time-of-flight mass spectrometry and ultra performance liquid chromatography quadrupole time-of-flight mass spectrometry. Consistent with our previous findings, we observed a number of dysregulated metabolic pathways, such as glycolysis, TCA cycle, urea cycle, pyrimidine metabolism, tryptophan metabolism, polyamine metabolism, as well as gut microbial-host co-metabolism in CRC subjects. Our findings confirm distinct urinary metabolic footprints of CRC patients characterized by altered levels of metabolites derived from gut microbial-host co-metabolism. A panel of metabolite markers composed of citrate, hippurate, p-cresol, 2-aminobutyrate, myristate, putrescine, and kynurenate was selected, which was able to discriminate CRC subjects from their healthy counterparts. A receiver operating characteristic curve (ROC) analysis of these markers resulted in an area under the receiver operating characteristic curve (AUC) of 0.993 and 0.998 for the training set and the testing set, respectively. These potential metabolite markers provide a novel and promising molecular diagnostic approach for the early detection of CRC. © 2011 American Chemical Society.
Persistent Identifierhttp://hdl.handle.net/10722/342729
ISSN
2023 Impact Factor: 3.8
2023 SCImago Journal Rankings: 1.299
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorCheng, Yu-
dc.contributor.authorXie, Guoxiang-
dc.contributor.authorChen, Tianlu-
dc.contributor.authorQiu, Yunping-
dc.contributor.authorZou, Xia-
dc.contributor.authorZheng, Minhua-
dc.contributor.authorTan, Binbin-
dc.contributor.authorFeng, Bo-
dc.contributor.authorDong, Taotao-
dc.contributor.authorHe, Pingang-
dc.contributor.authorZhao, Linjing-
dc.contributor.authorZhao, Aihua-
dc.contributor.authorXu, Lisa X.-
dc.contributor.authorZhang, Yan-
dc.contributor.authorJia, Wei-
dc.date.accessioned2024-04-17T07:05:51Z-
dc.date.available2024-04-17T07:05:51Z-
dc.date.issued2012-
dc.identifier.citationJournal of Proteome Research, 2012, v. 11, n. 2, p. 1354-1363-
dc.identifier.issn1535-3893-
dc.identifier.urihttp://hdl.handle.net/10722/342729-
dc.description.abstractA full spectrum of metabolic aberrations that are directly linked to colorectal cancer (CRC) at early curable stages is critical for developing and deploying molecular diagnostic and therapeutic approaches that will significantly improve patient survival. We have recently reported a urinary metabonomic profiling study on CRC subjects (n = 60) and health controls (n = 63), in which a panel of urinary metabolite markers was identified. Here, we report a second urinary metabonomic study on a larger cohort of CRC (n = 101) and healthy subjects (n = 103), using gas chromatography time-of-flight mass spectrometry and ultra performance liquid chromatography quadrupole time-of-flight mass spectrometry. Consistent with our previous findings, we observed a number of dysregulated metabolic pathways, such as glycolysis, TCA cycle, urea cycle, pyrimidine metabolism, tryptophan metabolism, polyamine metabolism, as well as gut microbial-host co-metabolism in CRC subjects. Our findings confirm distinct urinary metabolic footprints of CRC patients characterized by altered levels of metabolites derived from gut microbial-host co-metabolism. A panel of metabolite markers composed of citrate, hippurate, p-cresol, 2-aminobutyrate, myristate, putrescine, and kynurenate was selected, which was able to discriminate CRC subjects from their healthy counterparts. A receiver operating characteristic curve (ROC) analysis of these markers resulted in an area under the receiver operating characteristic curve (AUC) of 0.993 and 0.998 for the training set and the testing set, respectively. These potential metabolite markers provide a novel and promising molecular diagnostic approach for the early detection of CRC. © 2011 American Chemical Society.-
dc.languageeng-
dc.relation.ispartofJournal of Proteome Research-
dc.subjectcolorectal cancer-
dc.subjectgas chromatography time-of-flight mass spectrometry-
dc.subjectmetabolic profiling-
dc.subjectmetabonomics-
dc.subjectultra performance liquid chromatography quadrupole time-of-flight mass spectrometry-
dc.subjecturine-
dc.titleDistinct urinary metabolic profile of human colorectal cancer-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1021/pr201001a-
dc.identifier.pmid22148915-
dc.identifier.scopuseid_2-s2.0-84863024905-
dc.identifier.volume11-
dc.identifier.issue2-
dc.identifier.spage1354-
dc.identifier.epage1363-
dc.identifier.eissn1535-3907-
dc.identifier.isiWOS:000300458300071-

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