File Download
There are no files associated with this item.
Links for fulltext
(May Require Subscription)
- Publisher Website: 10.4161/cbt.4.6.1721
- Scopus: eid_2-s2.0-25144523473
- PMID: 15908782
- Find via
Supplementary
- Citations:
- Appears in Collections:
Article: Differential anti-tumor activity of Coriolus versicolor (Yunzhi) extract through p53- and/or Bcl-2-dependent apoptotic pathway in human breast cancer cells
Title | Differential anti-tumor activity of Coriolus versicolor (Yunzhi) extract through p53- and/or Bcl-2-dependent apoptotic pathway in human breast cancer cells |
---|---|
Authors | |
Keywords | Anti-tumor Apoptosis Bcl-2 Breast cancer cells Coriolus versicolor p53 Yunzhi |
Issue Date | 2005 |
Citation | Cancer Biology and Therapy, 2005, v. 4, n. 6, p. 638-644 How to Cite? |
Abstract | Coriolus versicolor (CV), also called Yunzhi, has been demonstrated to exert anti-tumor effects on various types of cancer cells, but the underlying mechanism has not been fully elucidated. The present study aimed to evaluate the in vitro anti-tumor activity of a standardized aqueous ethanol extract prepared from CV on four breast cancer cell lines using MTT assay, and test whether the mechanism involves apoptosis induction and modulation of p53 and Bcl-2 protein expressions using cell death detection ELISA, p53 and Bcl-2 ELISAs respectively. Our results demonstrated that the CV extract dose-dependently suppressed the proliferation of three breast tumor cell lines, with ascending order of IC 50 values: T-47D, MCF-7, MDA-MB-231, while BT-20 cells were not significantly affected. Tumoricidal activity of the CV extract was found to be comparable to a chemotherapeutic anti-cancer drug, mitomycin C. Nucleosome productions in apoptotic MDA-MB-231, MCF-7 and T-47D cells were significantly augmented in a time-dependent manner and paralleled the anti-proliferative activity of CV extract. Expression of p53 protein was significantly upregulated only in T-47D cells treated with the CV extract in a dose- and time-dependent fashion, but not in MCF-7 (except at 400 μg/ml after 16 h) and MDA-MB-231 cells. The CV extract significantly induced a dose-dependent downregulation of Bcl-2 protein expression in MCF-7 and T-47D cells, but not in MDA-MB-231 cells. These results suggested that apoptosis induction, differentially dependent of p53 and Bcl-2 expressions, might be the possible mechanism of CV extract-mediated cytotoxicity in human breast cancer cells in vitro. ©2005 Landes Bioscience. |
Persistent Identifier | http://hdl.handle.net/10722/343010 |
ISSN | 2023 Impact Factor: 4.4 2023 SCImago Journal Rankings: 0.914 |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ho, Cheong Yip | - |
dc.contributor.author | Kim, Chi Fai | - |
dc.contributor.author | Leung, Kwok Nam | - |
dc.contributor.author | Fung, Kwok Pui | - |
dc.contributor.author | Tse, Tak Fu | - |
dc.contributor.author | Chan, Helen | - |
dc.contributor.author | Lau, Clara Bik San | - |
dc.date.accessioned | 2024-05-10T09:04:45Z | - |
dc.date.available | 2024-05-10T09:04:45Z | - |
dc.date.issued | 2005 | - |
dc.identifier.citation | Cancer Biology and Therapy, 2005, v. 4, n. 6, p. 638-644 | - |
dc.identifier.issn | 1538-4047 | - |
dc.identifier.uri | http://hdl.handle.net/10722/343010 | - |
dc.description.abstract | Coriolus versicolor (CV), also called Yunzhi, has been demonstrated to exert anti-tumor effects on various types of cancer cells, but the underlying mechanism has not been fully elucidated. The present study aimed to evaluate the in vitro anti-tumor activity of a standardized aqueous ethanol extract prepared from CV on four breast cancer cell lines using MTT assay, and test whether the mechanism involves apoptosis induction and modulation of p53 and Bcl-2 protein expressions using cell death detection ELISA, p53 and Bcl-2 ELISAs respectively. Our results demonstrated that the CV extract dose-dependently suppressed the proliferation of three breast tumor cell lines, with ascending order of IC 50 values: T-47D, MCF-7, MDA-MB-231, while BT-20 cells were not significantly affected. Tumoricidal activity of the CV extract was found to be comparable to a chemotherapeutic anti-cancer drug, mitomycin C. Nucleosome productions in apoptotic MDA-MB-231, MCF-7 and T-47D cells were significantly augmented in a time-dependent manner and paralleled the anti-proliferative activity of CV extract. Expression of p53 protein was significantly upregulated only in T-47D cells treated with the CV extract in a dose- and time-dependent fashion, but not in MCF-7 (except at 400 μg/ml after 16 h) and MDA-MB-231 cells. The CV extract significantly induced a dose-dependent downregulation of Bcl-2 protein expression in MCF-7 and T-47D cells, but not in MDA-MB-231 cells. These results suggested that apoptosis induction, differentially dependent of p53 and Bcl-2 expressions, might be the possible mechanism of CV extract-mediated cytotoxicity in human breast cancer cells in vitro. ©2005 Landes Bioscience. | - |
dc.language | eng | - |
dc.relation.ispartof | Cancer Biology and Therapy | - |
dc.subject | Anti-tumor | - |
dc.subject | Apoptosis | - |
dc.subject | Bcl-2 | - |
dc.subject | Breast cancer cells | - |
dc.subject | Coriolus versicolor | - |
dc.subject | p53 | - |
dc.subject | Yunzhi | - |
dc.title | Differential anti-tumor activity of Coriolus versicolor (Yunzhi) extract through p53- and/or Bcl-2-dependent apoptotic pathway in human breast cancer cells | - |
dc.type | Article | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.4161/cbt.4.6.1721 | - |
dc.identifier.pmid | 15908782 | - |
dc.identifier.scopus | eid_2-s2.0-25144523473 | - |
dc.identifier.volume | 4 | - |
dc.identifier.issue | 6 | - |
dc.identifier.spage | 638 | - |
dc.identifier.epage | 644 | - |
dc.identifier.eissn | 1555-8576 | - |