Anti-angiogenic effects of the fruit of Alpinia oxyphylla

Abstract

Aim of study: The fruit of Alpinia oxyphylla, an herb commonly used in East Asian medicine, is variously used for the treatment of cancer and inflammatory conditions, which may possibly be mediated through anti-angiogenesis. This study aims to check for anti-angiogenic functions in the herb. Materials and methods: The 95% ethanol extract and four subsequent fractions (n-hexane, ethyl acetate, n-butanol and aqueous fractions) of the fruit of A. oxyphylla were tested on zebrafish model by quantitative endogenous alkaline phosphatase assay; then the active fractions were further tested on wild type and Tg(fli1a:EGFP)y1 zebrafish embryos and human umbilical vein endothelial cells and tumor cell lines for the anti-angiogenic effects. Results: The n-hexane and ethyl acetate fractions showed anti-angiogenic potentials in both in vivo and in vitro models. Conclusions: The use of A. oxyphylla for cancer and inflammation diseases may be partly due to its effects against vessel formation. © 2010 Elsevier Ireland Ltd.