File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Quantitative comparison of genetically encoded Ca 2+ indicators in cortical pyramidal cells and cerebellar purkinje cells

TitleQuantitative comparison of genetically encoded Ca <sup>2+</sup> indicators in cortical pyramidal cells and cerebellar purkinje cells
Authors
KeywordsAcute brain slice
Adenovirus
Cerebellar purkinje cell
Cortical pyramidal cell
Genetically encoded Ca indicators 2+
Patch-clamp recording
Two-photon imaging
Issue Date2011
Citation
Frontiers in Cellular Neuroscience, 2011, n. SEPTEMBER, p. 1-10 How to Cite?
AbstractGenetically encoded Ca 2+ indicators (GECIs) are promising tools for cell type-specific and chronic recording of neuronal activity. In the mammalian central nervous system, however, GECIs have been tested almost exclusively in cortical and hippocampal pyramidal cells, and the usefulness of recently developed GECIs has not been systematically examined in other cell types. Here we expressed the latest series of GECIs, yellow cameleon (YC) 2.60, YC3.60, YC-Nano15, and GCaMP3, in mouse cortical pyramidal cells as well as cerebellar Purkinje cells using in utero injection of recombinant adenoviral vectors. We characterized the performance of the GECIs by simultaneous two-photon imaging and whole-cell patchclamp recording in acute brain slices at 33±2°C. The fluorescent responses of GECIs to action potentials (APs) evoked by somatic current injection or to synaptic stimulation were examined using rapid dendritic imaging. In cortical pyramidal cells, YC2.60 showed the largest responses to single APs, but its decay kinetics were slower than YC3.60 and GCaMP3, while GCaMP3 showed the largest responses to 20APs evoked at 20Hz. In cerebellar Purkinje cells, onlyYC2.60 andYC-Nano15 could reliably report single complex spikes (CSs), and neither showed signal saturation over the entire stimulus range tested (1-10 CSs at 10Hz).The expression and response ofYC2.60 in Purkinje cells remained detectable and comparable for at least over 100days.These results provide useful information for selecting an optimal GECI depending on the experimental requirements: in cortical pyramidal cells, YC2.60 is suitable for detecting sparse firing of APs, whereas GCaMP3 is suitable for detecting burst firing of APs; in cerebellar Purkinje cells, YC2.60 as well as YC-Nano15 is suitable for detecting CSs. © 2011Yamada, Michikawa, Hashimoto, Horikawa, Nagai, Miyawaki, Häusserand Mikoshiba.
Persistent Identifierhttp://hdl.handle.net/10722/343098
ISSN
2023 Impact Factor: 4.2
2023 SCImago Journal Rankings: 1.471

 

DC FieldValueLanguage
dc.contributor.authorYamada, Yoshiyuki-
dc.contributor.authorMichikawa, Takayuki-
dc.contributor.authorHashimoto, Mitsuhiro-
dc.contributor.authorHorikawa, Kazuki-
dc.contributor.authorNagai, Takeharu-
dc.contributor.authorMiyawaki, Atsushi-
dc.contributor.authorHäusser, Michael-
dc.contributor.authorMikoshiba, Katsuhiko-
dc.date.accessioned2024-05-10T09:05:25Z-
dc.date.available2024-05-10T09:05:25Z-
dc.date.issued2011-
dc.identifier.citationFrontiers in Cellular Neuroscience, 2011, n. SEPTEMBER, p. 1-10-
dc.identifier.issn1662-5102-
dc.identifier.urihttp://hdl.handle.net/10722/343098-
dc.description.abstractGenetically encoded Ca 2+ indicators (GECIs) are promising tools for cell type-specific and chronic recording of neuronal activity. In the mammalian central nervous system, however, GECIs have been tested almost exclusively in cortical and hippocampal pyramidal cells, and the usefulness of recently developed GECIs has not been systematically examined in other cell types. Here we expressed the latest series of GECIs, yellow cameleon (YC) 2.60, YC3.60, YC-Nano15, and GCaMP3, in mouse cortical pyramidal cells as well as cerebellar Purkinje cells using in utero injection of recombinant adenoviral vectors. We characterized the performance of the GECIs by simultaneous two-photon imaging and whole-cell patchclamp recording in acute brain slices at 33±2°C. The fluorescent responses of GECIs to action potentials (APs) evoked by somatic current injection or to synaptic stimulation were examined using rapid dendritic imaging. In cortical pyramidal cells, YC2.60 showed the largest responses to single APs, but its decay kinetics were slower than YC3.60 and GCaMP3, while GCaMP3 showed the largest responses to 20APs evoked at 20Hz. In cerebellar Purkinje cells, onlyYC2.60 andYC-Nano15 could reliably report single complex spikes (CSs), and neither showed signal saturation over the entire stimulus range tested (1-10 CSs at 10Hz).The expression and response ofYC2.60 in Purkinje cells remained detectable and comparable for at least over 100days.These results provide useful information for selecting an optimal GECI depending on the experimental requirements: in cortical pyramidal cells, YC2.60 is suitable for detecting sparse firing of APs, whereas GCaMP3 is suitable for detecting burst firing of APs; in cerebellar Purkinje cells, YC2.60 as well as YC-Nano15 is suitable for detecting CSs. © 2011Yamada, Michikawa, Hashimoto, Horikawa, Nagai, Miyawaki, Häusserand Mikoshiba.-
dc.languageeng-
dc.relation.ispartofFrontiers in Cellular Neuroscience-
dc.subjectAcute brain slice-
dc.subjectAdenovirus-
dc.subjectCerebellar purkinje cell-
dc.subjectCortical pyramidal cell-
dc.subjectGenetically encoded Ca indicators 2+-
dc.subjectPatch-clamp recording-
dc.subjectTwo-photon imaging-
dc.titleQuantitative comparison of genetically encoded Ca <sup>2+</sup> indicators in cortical pyramidal cells and cerebellar purkinje cells-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.3389/fncel.2011.00018-
dc.identifier.scopuseid_2-s2.0-84862600723-
dc.identifier.issueSEPTEMBER-
dc.identifier.spage1-
dc.identifier.epage10-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats