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- Publisher Website: 10.7554/eLife.05808
- Scopus: eid_2-s2.0-84926500475
- PMID: 25824291
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Article: CaRuby-Nano: A novel high affinity calcium probe for dual color imaging
| Title | CaRuby-Nano: A novel high affinity calcium probe for dual color imaging |
|---|---|
| Authors | |
| Issue Date | 2015 |
| Citation | eLife, 2015, v. 2015, n. 4, article no. e05808 How to Cite? |
| Abstract | The great demand for long-wavelength and high signal-to-noise Ca2+ indicators has led us to develop CaRuby-Nano, a new functionalizable red calcium indicator with nanomolar affinity for use in cell biology and neuroscience research. In addition, we generated CaRuby-Nano dextran conjugates and an AM-ester variant for bulk loading of tissue. We tested the new indicator using in vitro and in vivo experiments demonstrating the high sensitivity of CaRuby-Nano as well as its power in dual color imaging experiments. |
| Persistent Identifier | http://hdl.handle.net/10722/343176 |
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | Collot, Mayeul | - |
| dc.contributor.author | Wilms, Christian D. | - |
| dc.contributor.author | Bentkhayet, Asma | - |
| dc.contributor.author | Marcaggi, Païkan | - |
| dc.contributor.author | Couchman, Kiri | - |
| dc.contributor.author | Charpak, Serge | - |
| dc.contributor.author | Dieudonné, Stéphane | - |
| dc.contributor.author | Häusser, Michael | - |
| dc.contributor.author | Feltz, Anne | - |
| dc.contributor.author | Mallet, Jean Maurice | - |
| dc.date.accessioned | 2024-05-10T09:06:02Z | - |
| dc.date.available | 2024-05-10T09:06:02Z | - |
| dc.date.issued | 2015 | - |
| dc.identifier.citation | eLife, 2015, v. 2015, n. 4, article no. e05808 | - |
| dc.identifier.uri | http://hdl.handle.net/10722/343176 | - |
| dc.description.abstract | The great demand for long-wavelength and high signal-to-noise Ca2+ indicators has led us to develop CaRuby-Nano, a new functionalizable red calcium indicator with nanomolar affinity for use in cell biology and neuroscience research. In addition, we generated CaRuby-Nano dextran conjugates and an AM-ester variant for bulk loading of tissue. We tested the new indicator using in vitro and in vivo experiments demonstrating the high sensitivity of CaRuby-Nano as well as its power in dual color imaging experiments. | - |
| dc.language | eng | - |
| dc.relation.ispartof | eLife | - |
| dc.title | CaRuby-Nano: A novel high affinity calcium probe for dual color imaging | - |
| dc.type | Article | - |
| dc.description.nature | link_to_subscribed_fulltext | - |
| dc.identifier.doi | 10.7554/eLife.05808 | - |
| dc.identifier.pmid | 25824291 | - |
| dc.identifier.scopus | eid_2-s2.0-84926500475 | - |
| dc.identifier.volume | 2015 | - |
| dc.identifier.issue | 4 | - |
| dc.identifier.spage | article no. e05808 | - |
| dc.identifier.epage | article no. e05808 | - |
| dc.identifier.eissn | 2050-084X | - |