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Article: The protective effect of Herba Cistanches on statin-induced myotoxicity in vitro

TitleThe protective effect of Herba Cistanches on statin-induced myotoxicity in vitro
Authors
KeywordsCaspase-3
Cholesterol
Herba Cistanches
Hyperlipidemia
Muscle toxicity
Statin
Verbascoside
Issue Date2016
Citation
Journal of Ethnopharmacology, 2016, v. 190, p. 68-73 How to Cite?
AbstractEthnopharmacological relevance Herba Cistanches (HC, Cistanche deserticola or Cistanche tubulosa) is a Chinese herb traditionally used for muscle problems. Previous studies demonstrated that HC extract could reduce muscle damage and improve ATP storage in post-exercised rats. However, its effect on statin-induced muscle toxicity has never been investigated. Aim The objective of this study was to determine if the aqueous extract of HC (HCE) could prevent simvastatin-induced toxicity in L6 rat skeletal muscle cells; and whether verbascoside is the major bioactive constituent which contributes to the effects. Materials and methods MTT was performed to determine the effects of HCE (0-2000 μg/ml) or verbascoside (0-160 μM) on simvastatin (10 μM)-treated L6 cells. Annexin V-FITC/PI apoptosis assay and Caspase 3 assay were performed to determine the protective role of HCE on simvastatin-induced cell death, and to evaluate if HCE exerted its protective effect through the caspase pathway. ATP production was measured to investigate if HCE could prevent simvastatin-induced reduction in ATP production in vitro. Results Simvastatin significantly increased apoptotic cell death in L6 cells. HCE significantly exerted a dose-dependent reduction on simvastatin-induced apoptotic cells, possibly via caspase-3 pathway. Simvastatin reduced the ATP production in L6 cells, which was dose-dependently prevented by HCE. There was only a trend but not significant effect (except at high dose) of verbascoside on the protection of simvastatin-induced muscle toxicity. Conclusions In conclusion, we demonstrated for the first time that HCE could exert dose-dependent protective effect on simvastatin-induced toxicity in vitro, which was unlikely due to the presence of verbascoside. Our study suggested the potential use of HC under the situation of simvastatin-induced muscle toxicity.
Persistent Identifierhttp://hdl.handle.net/10722/343215
ISSN
2023 Impact Factor: 4.8
2023 SCImago Journal Rankings: 0.936

 

DC FieldValueLanguage
dc.contributor.authorWat, Elaine-
dc.contributor.authorNg, Chun Fai-
dc.contributor.authorKoon, Chi Man-
dc.contributor.authorWong, Eric Chun Wai-
dc.contributor.authorTomlinson, Brian-
dc.contributor.authorLau, Clara Bik San-
dc.date.accessioned2024-05-10T09:06:21Z-
dc.date.available2024-05-10T09:06:21Z-
dc.date.issued2016-
dc.identifier.citationJournal of Ethnopharmacology, 2016, v. 190, p. 68-73-
dc.identifier.issn0378-8741-
dc.identifier.urihttp://hdl.handle.net/10722/343215-
dc.description.abstractEthnopharmacological relevance Herba Cistanches (HC, Cistanche deserticola or Cistanche tubulosa) is a Chinese herb traditionally used for muscle problems. Previous studies demonstrated that HC extract could reduce muscle damage and improve ATP storage in post-exercised rats. However, its effect on statin-induced muscle toxicity has never been investigated. Aim The objective of this study was to determine if the aqueous extract of HC (HCE) could prevent simvastatin-induced toxicity in L6 rat skeletal muscle cells; and whether verbascoside is the major bioactive constituent which contributes to the effects. Materials and methods MTT was performed to determine the effects of HCE (0-2000 μg/ml) or verbascoside (0-160 μM) on simvastatin (10 μM)-treated L6 cells. Annexin V-FITC/PI apoptosis assay and Caspase 3 assay were performed to determine the protective role of HCE on simvastatin-induced cell death, and to evaluate if HCE exerted its protective effect through the caspase pathway. ATP production was measured to investigate if HCE could prevent simvastatin-induced reduction in ATP production in vitro. Results Simvastatin significantly increased apoptotic cell death in L6 cells. HCE significantly exerted a dose-dependent reduction on simvastatin-induced apoptotic cells, possibly via caspase-3 pathway. Simvastatin reduced the ATP production in L6 cells, which was dose-dependently prevented by HCE. There was only a trend but not significant effect (except at high dose) of verbascoside on the protection of simvastatin-induced muscle toxicity. Conclusions In conclusion, we demonstrated for the first time that HCE could exert dose-dependent protective effect on simvastatin-induced toxicity in vitro, which was unlikely due to the presence of verbascoside. Our study suggested the potential use of HC under the situation of simvastatin-induced muscle toxicity.-
dc.languageeng-
dc.relation.ispartofJournal of Ethnopharmacology-
dc.subjectCaspase-3-
dc.subjectCholesterol-
dc.subjectHerba Cistanches-
dc.subjectHyperlipidemia-
dc.subjectMuscle toxicity-
dc.subjectStatin-
dc.subjectVerbascoside-
dc.titleThe protective effect of Herba Cistanches on statin-induced myotoxicity in vitro-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.jep.2016.06.020-
dc.identifier.pmid27286913-
dc.identifier.scopuseid_2-s2.0-84973550045-
dc.identifier.volume190-
dc.identifier.spage68-
dc.identifier.epage73-
dc.identifier.eissn1872-7573-

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