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Article: Anti-inflammatory activities of natural cyclopeptide RA-XII in colitis-associated colon cancer mouse model and its effect on gut microbiome

TitleAnti-inflammatory activities of natural cyclopeptide RA-XII in colitis-associated colon cancer mouse model and its effect on gut microbiome
Authors
Keywordscolitis
colorectal cancer
gut microbiome
inflammatory
microenvironment
RA-XII
Issue Date2022
Citation
Phytotherapy Research, 2022, v. 36, n. 6, p. 2641-2659 How to Cite?
AbstractColorectal cancer (CRC), the third most common cancer globally, is associated with intestinal inflammation that leads to poor prognosis. RA-XII, a natural cyclopeptide, has previously been reported to possess anti-tumor activities. Here, the anti-inflammatory activities of RA-XII were investigated in colitis-associated colon cancer mice and a co-culture in vitro model, in which colon cancer cells HCT116 and macrophages RAW264.7 were grown together to mimic the inflammatory microenvironment of CRC. Changes of inflammatory-related molecules and protein expressions in cells were evaluated after RA-XII incubation. Besides, azoxymethane and dextran sulfate sodium-induced colitis-associated colon cancer mice were treated with RA-XII for 24 days, inflammatory parameters and gut microbiome alterations were studied. Our results showed that RA-XII reversed the inflammatory responses of RAW264.7 cells induced by LPS and modulated the protein expressions of AKT, STAT3/p-STAT3, P70S6K, NF-κB and GSK3β and suppressed the expression of LC3A/B in HCT116 cells in co-culture system. RA-XII treatment restored the colitis damage in colon, reduced colon tumors numbers and decreased inflammatory factors (IL-6, IL-10 and TNF-α). The role of RA-XII on regulating gut microbiome was also demonstrated for the first time. In conclusion, our findings provided new scientific evidence for developing RA-XII as a potent anti-inflammatory agent for CRC.
Persistent Identifierhttp://hdl.handle.net/10722/343373
ISSN
2023 Impact Factor: 6.1
2023 SCImago Journal Rankings: 1.277

 

DC FieldValueLanguage
dc.contributor.authorLin, Qianwen-
dc.contributor.authorLiu, Mingyu-
dc.contributor.authorYue, Grace Gar Lee-
dc.contributor.authorCheung, Man Kit-
dc.contributor.authorLai, Zhixing-
dc.contributor.authorKwok, Frankie Hin Fai-
dc.contributor.authorLee, Julia Kin Ming-
dc.contributor.authorWang, Zhe-
dc.contributor.authorLau, Clara Bik San-
dc.contributor.authorTan, Ninghua-
dc.date.accessioned2024-05-10T09:07:34Z-
dc.date.available2024-05-10T09:07:34Z-
dc.date.issued2022-
dc.identifier.citationPhytotherapy Research, 2022, v. 36, n. 6, p. 2641-2659-
dc.identifier.issn0951-418X-
dc.identifier.urihttp://hdl.handle.net/10722/343373-
dc.description.abstractColorectal cancer (CRC), the third most common cancer globally, is associated with intestinal inflammation that leads to poor prognosis. RA-XII, a natural cyclopeptide, has previously been reported to possess anti-tumor activities. Here, the anti-inflammatory activities of RA-XII were investigated in colitis-associated colon cancer mice and a co-culture in vitro model, in which colon cancer cells HCT116 and macrophages RAW264.7 were grown together to mimic the inflammatory microenvironment of CRC. Changes of inflammatory-related molecules and protein expressions in cells were evaluated after RA-XII incubation. Besides, azoxymethane and dextran sulfate sodium-induced colitis-associated colon cancer mice were treated with RA-XII for 24 days, inflammatory parameters and gut microbiome alterations were studied. Our results showed that RA-XII reversed the inflammatory responses of RAW264.7 cells induced by LPS and modulated the protein expressions of AKT, STAT3/p-STAT3, P70S6K, NF-κB and GSK3β and suppressed the expression of LC3A/B in HCT116 cells in co-culture system. RA-XII treatment restored the colitis damage in colon, reduced colon tumors numbers and decreased inflammatory factors (IL-6, IL-10 and TNF-α). The role of RA-XII on regulating gut microbiome was also demonstrated for the first time. In conclusion, our findings provided new scientific evidence for developing RA-XII as a potent anti-inflammatory agent for CRC.-
dc.languageeng-
dc.relation.ispartofPhytotherapy Research-
dc.subjectcolitis-
dc.subjectcolorectal cancer-
dc.subjectgut microbiome-
dc.subjectinflammatory-
dc.subjectmicroenvironment-
dc.subjectRA-XII-
dc.titleAnti-inflammatory activities of natural cyclopeptide RA-XII in colitis-associated colon cancer mouse model and its effect on gut microbiome-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/ptr.7482-
dc.identifier.pmid35537703-
dc.identifier.scopuseid_2-s2.0-85129747690-
dc.identifier.volume36-
dc.identifier.issue6-
dc.identifier.spage2641-
dc.identifier.epage2659-
dc.identifier.eissn1099-1573-

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