File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: WT1 as a myoepithelial marker: a comparative study of breast, cutaneous, and salivary gland lesions

TitleWT1 as a myoepithelial marker: a comparative study of breast, cutaneous, and salivary gland lesions
Authors
KeywordsAdenoid cystic carcinoma
Cutaneous mixed tumor
Myoepithelium
Pleomorphic adenoma
WT1
Issue Date2023
Citation
Human Pathology, 2023, v. 135, p. 76-83 How to Cite?
AbstractWT1 immunostain is expressed in various benign and malignant neoplasms, as well as normal myoepithelial cells. WT1 shows differential expression in non-neoplastic, benign, and malignant neoplastic myoepithelial cells of the salivary gland. In this study, WT1 immunostain and other myoepithelial markers were compared to investigate the value of WT1 as a myoepithelial marker, and to delineate the expression profile of WT1 in nonsalivary gland myoepithelial cells. WT1, p63, and calponin immunostains were performed on normal and lesional tissues from the breast (adenosis, sclerosing adenosis, lactating adenoma, nipple adenoma, tubular adenoma, adenomyoepithelioma, and adenoid cystic carcinoma [ACC]), skin (cutaneous mixed tumor, hidradenoma, spiradenoma, and ACC), and salivary gland (pleomorphic adenoma and ACC). The stained slides were digitized and orientated with H&E images and assessed simultaneously using QuPath. A total of 129, 58, and 56 breast, cutaneous, and salivary gland lesions, respectively, were included. There was poor agreement between WT1-p63 and WT1-calponin (κ < 0.1) in all organs, with absence of WT1 expression in normal salivary gland myoepithelium and most ACCs. There were no significant differences in WT1 expression in myoepithelial cells in normal breast tissue and benign breast neoplasms. Compared to pleomorphic adenomas, cutaneous mixed tumors showed lower WT1 expression (P < .001). WT1 is a less sensitive myoepithelial marker than calponin and p63. However, its unique pattern of expression in salivary gland primary for pleomorphic adenomas/cutaneous mixed tumor can favor a diagnosis of benign salivary gland tumors, particularly in small biopsy specimens.
Persistent Identifierhttp://hdl.handle.net/10722/343411
ISSN
2023 Impact Factor: 2.7
2023 SCImago Journal Rankings: 0.936

 

DC FieldValueLanguage
dc.contributor.authorNg, Joanna K.M.-
dc.contributor.authorLi, Joshua J.X.-
dc.contributor.authorLai, Billy S.W.-
dc.contributor.authorTsang, Julia Y.-
dc.contributor.authorChan, Agnes W.S.-
dc.contributor.authorCheung, Christina M.T.-
dc.contributor.authorIp, Edric C.C.-
dc.contributor.authorTse, Gary M.-
dc.date.accessioned2024-05-10T09:07:55Z-
dc.date.available2024-05-10T09:07:55Z-
dc.date.issued2023-
dc.identifier.citationHuman Pathology, 2023, v. 135, p. 76-83-
dc.identifier.issn0046-8177-
dc.identifier.urihttp://hdl.handle.net/10722/343411-
dc.description.abstractWT1 immunostain is expressed in various benign and malignant neoplasms, as well as normal myoepithelial cells. WT1 shows differential expression in non-neoplastic, benign, and malignant neoplastic myoepithelial cells of the salivary gland. In this study, WT1 immunostain and other myoepithelial markers were compared to investigate the value of WT1 as a myoepithelial marker, and to delineate the expression profile of WT1 in nonsalivary gland myoepithelial cells. WT1, p63, and calponin immunostains were performed on normal and lesional tissues from the breast (adenosis, sclerosing adenosis, lactating adenoma, nipple adenoma, tubular adenoma, adenomyoepithelioma, and adenoid cystic carcinoma [ACC]), skin (cutaneous mixed tumor, hidradenoma, spiradenoma, and ACC), and salivary gland (pleomorphic adenoma and ACC). The stained slides were digitized and orientated with H&E images and assessed simultaneously using QuPath. A total of 129, 58, and 56 breast, cutaneous, and salivary gland lesions, respectively, were included. There was poor agreement between WT1-p63 and WT1-calponin (κ < 0.1) in all organs, with absence of WT1 expression in normal salivary gland myoepithelium and most ACCs. There were no significant differences in WT1 expression in myoepithelial cells in normal breast tissue and benign breast neoplasms. Compared to pleomorphic adenomas, cutaneous mixed tumors showed lower WT1 expression (P < .001). WT1 is a less sensitive myoepithelial marker than calponin and p63. However, its unique pattern of expression in salivary gland primary for pleomorphic adenomas/cutaneous mixed tumor can favor a diagnosis of benign salivary gland tumors, particularly in small biopsy specimens.-
dc.languageeng-
dc.relation.ispartofHuman Pathology-
dc.subjectAdenoid cystic carcinoma-
dc.subjectCutaneous mixed tumor-
dc.subjectMyoepithelium-
dc.subjectPleomorphic adenoma-
dc.subjectWT1-
dc.titleWT1 as a myoepithelial marker: a comparative study of breast, cutaneous, and salivary gland lesions-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.humpath.2023.01.005-
dc.identifier.pmid36739952-
dc.identifier.scopuseid_2-s2.0-85149636718-
dc.identifier.volume135-
dc.identifier.spage76-
dc.identifier.epage83-
dc.identifier.eissn1532-8392-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats