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Article: Optimizing non-invasive preimplantation genetic testing: investigating culture conditions, sample collection, and IVF treatment for improved non-invasive PGT-A results

TitleOptimizing non-invasive preimplantation genetic testing: investigating culture conditions, sample collection, and IVF treatment for improved non-invasive PGT-A results
Authors
KeywordsAneuploidy
niPGT-A
Non-invasive
PGT-A
Spent culture media
Issue Date6-Jan-2024
PublisherSpringer
Citation
Journal of Assisted Reproduction and Genetics, 2024, v. 41, n. 2, p. 465-472 How to Cite?
Abstract

Purpose: This study aimed to optimize the non-invasive preimplantation genetic testing for aneuploidy (niPGT-A) in the laboratory by comparing two collection timing of the spent culture medium (SCM), two embryo rinsing protocols, and the use of conventional insemination instead of intracytoplasmic sperm injection (ICSI). Methods: Results of two embryo rinsing methods (one-step vs sequential) and SCM collected on day 5 vs day 6 after retrieval were compared against trophectoderm (TE) biopsies as reference. Results from day 6 SCM in cycles fertilized by conventional insemination were compared with PGT-A using ICSI. Results: The rate of concordance was higher in day 6 samples than in day 5 samples when the sequential method was used, in terms of total concordance (TC; day 6 vs day 5: 85.0% vs 60.0%, p = 0.0228), total concordance with same sex (TCS, 82.5% vs 28,0%, p < 0.0001), and full concordance with same sex (FCS, 62.5% vs 24.0%, p = 0.0025). The sequential method significantly out-performed the one-step method when SCM were collected on day 6 (sequential vs one-step, TC: 85.0% vs 64.5%, p = 0.0449; TCS: 82.5% vs 54.8%, p = 0.0113; FCS: 62.5% vs 25.8%, p = 0.0021). There was no significant difference in niPGT-A results between cycles fertilized by the conventional insemination and ICSI. Conclusion: We have shown a higher concordance rate when SCM was collected on day 6 and the embryos were rinsed in a sequential manner. Comparable results of niPGT-A when oocytes were fertilized by conventional insemination or ICSI. These optimization steps are important prior to commencement of a randomized trial in niPGT-A.


Persistent Identifierhttp://hdl.handle.net/10722/343556
ISSN
2023 Impact Factor: 3.2
2023 SCImago Journal Rankings: 1.004

 

DC FieldValueLanguage
dc.contributor.authorChow, Judy F C-
dc.contributor.authorLam, Kevin K W-
dc.contributor.authorCheng, Heidi H Y-
dc.contributor.authorLai, Shui Fan-
dc.contributor.authorYeung, William S B-
dc.contributor.authorNg, Ernest H Y-
dc.date.accessioned2024-05-21T03:11:45Z-
dc.date.available2024-05-21T03:11:45Z-
dc.date.issued2024-01-06-
dc.identifier.citationJournal of Assisted Reproduction and Genetics, 2024, v. 41, n. 2, p. 465-472-
dc.identifier.issn1058-0468-
dc.identifier.urihttp://hdl.handle.net/10722/343556-
dc.description.abstract<p>Purpose: This study aimed to optimize the non-invasive preimplantation genetic testing for aneuploidy (niPGT-A) in the laboratory by comparing two collection timing of the spent culture medium (SCM), two embryo rinsing protocols, and the use of conventional insemination instead of intracytoplasmic sperm injection (ICSI). Methods: Results of two embryo rinsing methods (one-step vs sequential) and SCM collected on day 5 vs day 6 after retrieval were compared against trophectoderm (TE) biopsies as reference. Results from day 6 SCM in cycles fertilized by conventional insemination were compared with PGT-A using ICSI. Results: The rate of concordance was higher in day 6 samples than in day 5 samples when the sequential method was used, in terms of total concordance (TC; day 6 vs day 5: 85.0% vs 60.0%, p = 0.0228), total concordance with same sex (TCS, 82.5% vs 28,0%, p &lt; 0.0001), and full concordance with same sex (FCS, 62.5% vs 24.0%, p = 0.0025). The sequential method significantly out-performed the one-step method when SCM were collected on day 6 (sequential vs one-step, TC: 85.0% vs 64.5%, p = 0.0449; TCS: 82.5% vs 54.8%, p = 0.0113; FCS: 62.5% vs 25.8%, p = 0.0021). There was no significant difference in niPGT-A results between cycles fertilized by the conventional insemination and ICSI. Conclusion: We have shown a higher concordance rate when SCM was collected on day 6 and the embryos were rinsed in a sequential manner. Comparable results of niPGT-A when oocytes were fertilized by conventional insemination or ICSI. These optimization steps are important prior to commencement of a randomized trial in niPGT-A.</p>-
dc.languageeng-
dc.publisherSpringer-
dc.relation.ispartofJournal of Assisted Reproduction and Genetics-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectAneuploidy-
dc.subjectniPGT-A-
dc.subjectNon-invasive-
dc.subjectPGT-A-
dc.subjectSpent culture media-
dc.titleOptimizing non-invasive preimplantation genetic testing: investigating culture conditions, sample collection, and IVF treatment for improved non-invasive PGT-A results-
dc.typeArticle-
dc.identifier.doi10.1007/s10815-023-03015-3-
dc.identifier.scopuseid_2-s2.0-85181467079-
dc.identifier.volume41-
dc.identifier.issue2-
dc.identifier.spage465-
dc.identifier.epage472-
dc.identifier.eissn1573-7330-
dc.identifier.issnl1058-0468-

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