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Article: A promoter polymorphism defines distinct roles in anther development for Col-0 and Ler-0 alleles of Arabidopsis ACYL-COA BINDING PROTEIN3
| Title | A promoter polymorphism defines distinct roles in anther development for Col-0 and Ler-0 alleles of Arabidopsis ACYL-COA BINDING PROTEIN3 |
|---|---|
| Authors | |
| Keywords | acyl-CoA thioesters anther locule EMSA ERECTA HP-LC luciferase assay promoter variations sporocyte formation |
| Issue Date | 24-Jun-2024 |
| Publisher | Wiley |
| Citation | New Phytologist, 2024, v. 243, n. 4, p. 1424-1439 How to Cite? |
| Abstract | Acyl-CoA-Binding Proteins (ACBPs) bind acyl-CoA esters and function in lipid metabolism. Although acbp3-1, the ACBP3 mutant in Arabidopsis thaliana ecotype Col-0, displays normal floral development, the acbp3-2 mutant from ecotype Ler-0 characterized herein exhibits defective adaxial anther lobes and improper sporocyte formation. To understand these differences and identify the role of ERECTA in ACBP3 function, the acbp3 mutants and acbp3-erecta (er) lines were analyzed by microscopy for anther morphology and high-performance liquid chromatography for lipid composition. Defects in Landsberg anther development were related to the ERECTA-mediated pathway because the progenies of acbp3-2 × La-0 and acbp3-1 × er-1 in Col-0 showed normal anthers, contrasting to that of acbp3-2 in Ler-0. Polymorphism in the regulatory region of ACBP3 enabled its function in anther development in Ler-0 but not Col-0 which harbored an AT-repeat insertion. ACBP3 expression and anther development in acbp3-2 were restored using ACBP3pro (Ler)::ACBP3 not ACBP3pro (Col)::ACBP3. SPOROCYTELESS (SPL), a sporocyte formation regulator activated ACBP3 transcription in Ler-0 but not Col-0. For anther development, the ERECTA-related role of ACBP3 is required in Ler-0, but not Col-0. The disrupted promoter regulatory region for SPL binding in Col-0 eliminates the role of ACBP3 in anther development. |
| Persistent Identifier | http://hdl.handle.net/10722/344328 |
| ISSN | 2023 Impact Factor: 8.3 2023 SCImago Journal Rankings: 3.007 |
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | Guo, Ze-Hua | - |
| dc.contributor.author | Hu, Tai-Hua | - |
| dc.contributor.author | Hamdan, Mohd Fadhli | - |
| dc.contributor.author | Li, Minghui | - |
| dc.contributor.author | Wang, Ruifeng | - |
| dc.contributor.author | Xu, Jie | - |
| dc.contributor.author | Lung, Shiu-Cheung | - |
| dc.contributor.author | Liang, Wanqi | - |
| dc.contributor.author | Shi, Jianxin | - |
| dc.contributor.author | Zhang, Dabing | - |
| dc.contributor.author | Chye, Mee-Len | - |
| dc.date.accessioned | 2024-07-24T13:50:46Z | - |
| dc.date.available | 2024-07-24T13:50:46Z | - |
| dc.date.issued | 2024-06-24 | - |
| dc.identifier.citation | New Phytologist, 2024, v. 243, n. 4, p. 1424-1439 | - |
| dc.identifier.issn | 0028-646X | - |
| dc.identifier.uri | http://hdl.handle.net/10722/344328 | - |
| dc.description.abstract | <p> <span>Acyl-CoA-Binding Proteins (ACBPs) bind acyl-CoA esters and function in lipid metabolism. Although acbp3-1, the ACBP3 mutant in Arabidopsis thaliana ecotype Col-0, displays normal floral development, the acbp3-2 mutant from ecotype Ler-0 characterized herein exhibits defective adaxial anther lobes and improper sporocyte formation. To understand these differences and identify the role of ERECTA in ACBP3 function, the acbp3 mutants and acbp3-erecta (er) lines were analyzed by microscopy for anther morphology and high-performance liquid chromatography for lipid composition. Defects in Landsberg anther development were related to the ERECTA-mediated pathway because the progenies of acbp3-2 × La-0 and acbp3-1 × er-1 in Col-0 showed normal anthers, contrasting to that of acbp3-2 in Ler-0. Polymorphism in the regulatory region of ACBP3 enabled its function in anther development in Ler-0 but not Col-0 which harbored an AT-repeat insertion. ACBP3 expression and anther development in acbp3-2 were restored using ACBP3pro (Ler)::ACBP3 not ACBP3pro (Col)::ACBP3. SPOROCYTELESS (SPL), a sporocyte formation regulator activated ACBP3 transcription in Ler-0 but not Col-0. For anther development, the ERECTA-related role of ACBP3 is required in Ler-0, but not Col-0. The disrupted promoter regulatory region for SPL binding in Col-0 eliminates the role of ACBP3 in anther development.</span> <br></p> | - |
| dc.language | eng | - |
| dc.publisher | Wiley | - |
| dc.relation.ispartof | New Phytologist | - |
| dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
| dc.subject | acyl-CoA thioesters | - |
| dc.subject | anther locule | - |
| dc.subject | EMSA | - |
| dc.subject | ERECTA | - |
| dc.subject | HP-LC | - |
| dc.subject | luciferase assay | - |
| dc.subject | promoter variations | - |
| dc.subject | sporocyte formation | - |
| dc.title | A promoter polymorphism defines distinct roles in anther development for Col-0 and Ler-0 alleles of Arabidopsis ACYL-COA BINDING PROTEIN3 | - |
| dc.type | Article | - |
| dc.description.nature | published_or_final_version | - |
| dc.identifier.doi | 10.1111/nph.19924 | - |
| dc.identifier.scopus | eid_2-s2.0-85196657318 | - |
| dc.identifier.volume | 243 | - |
| dc.identifier.issue | 4 | - |
| dc.identifier.spage | 1424 | - |
| dc.identifier.epage | 1439 | - |
| dc.identifier.eissn | 1469-8137 | - |
| dc.identifier.issnl | 0028-646X | - |