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- Publisher Website: 10.1016/j.bj.2022.10.003
- Scopus: eid_2-s2.0-85149107343
- PMID: 36244649
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Article: The effects of intermittent hypoxia on hepatic expression of fatty acid translocase CD36 in lean and diet-induced obese mice
Title | The effects of intermittent hypoxia on hepatic expression of fatty acid translocase CD36 in lean and diet-induced obese mice |
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Authors | |
Keywords | Inflammation Intermittent hypoxia Liver injury Obstructive sleep apnea Oxidative stress |
Issue Date | 1-Oct-2023 |
Publisher | Elsevier |
Citation | Biomedical Journal, 2023, v. 46, n. 5 How to Cite? |
Abstract | Background: Both obstructive sleep apnea (OSA) and non-alcoholic fatty liver disease (NAFLD) are prevalent within obese individuals. We aimed to investigate the effects of intermittent hypoxia (IH), a clinical feature of OSA, on hepatic expression of fatty acid translocase (CD36) in relation to liver injury in lean and diet-induced obese mice. Methods: Four-week-old male C57BL/6J mice were randomized to standard diet (SD) or high fat (HF) diet groups. At 13-week-old, all mice were exposed to either air or IH (IH30; thirty hypoxic episodes per hour) for four weeks. We assessed liver injury through lipid profile, oxidative and inflammatory stress, histological scoring and hepatic CD36 expression. Results: In lean mice, IH elevated serum and hepatic triglyceride and free fatty acid (FFA) levels, in line with upregulation of hepatic CD36 expression and myeloperoxidase (MPO)-positive cells in support of inflammatory infiltrates along with increase in serum malondialdehyde (MDA), C-X-C motif chemokine ligand 1(CXCL-1) and monocyte chemoattractant protein-1 (MCP-1). In diet-induced obese mice, an increase in hepatic alanine transaminase (ALT) activity, serum and hepatic levels of lipid parameters and inflammatory markers, serum MDA level, hepatic expressions of CD36 and α-smooth muscle actin (α-SMA), and MPO-positive cells was observed. IH potentiated hepatic ALT activity, serum CXCL-1 and hepatic interleukin-6 (IL-6), in line with inflammatory infiltrates, but paradoxically, reduced hepatic FFA level and hepatic CD36 expression, compared to obese mice without IH exposure. However, IH further augmented diet-induced liver steatosis and fibrosis as shown by histological scores. Conclusion: This study contributes to support that IH featuring OSA may lead to liver injury via differential regulation of hepatic CD36 expression in lean and diet-induced obese mice. |
Persistent Identifier | http://hdl.handle.net/10722/344948 |
ISSN | 2023 Impact Factor: 4.1 2023 SCImago Journal Rankings: 1.143 |
DC Field | Value | Language |
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dc.contributor.author | Ji, Yang | - |
dc.contributor.author | Liang, Yingmin | - |
dc.contributor.author | Chu, Pak Hin | - |
dc.contributor.author | Ge, Mengqin | - |
dc.contributor.author | Yeung, Sze Chun | - |
dc.contributor.author | Ip, Mary Sau Man | - |
dc.contributor.author | Mak, Judith Choi Wo | - |
dc.date.accessioned | 2024-08-14T08:56:27Z | - |
dc.date.available | 2024-08-14T08:56:27Z | - |
dc.date.issued | 2023-10-01 | - |
dc.identifier.citation | Biomedical Journal, 2023, v. 46, n. 5 | - |
dc.identifier.issn | 2319-4170 | - |
dc.identifier.uri | http://hdl.handle.net/10722/344948 | - |
dc.description.abstract | <p>Background: Both obstructive sleep apnea (OSA) and non-alcoholic fatty liver disease (NAFLD) are prevalent within obese individuals. We aimed to investigate the effects of intermittent hypoxia (IH), a clinical feature of OSA, on hepatic expression of fatty acid translocase (CD36) in relation to liver injury in lean and diet-induced obese mice. Methods: Four-week-old male C57BL/6J mice were randomized to standard diet (SD) or high fat (HF) diet groups. At 13-week-old, all mice were exposed to either air or IH (IH30; thirty hypoxic episodes per hour) for four weeks. We assessed liver injury through lipid profile, oxidative and inflammatory stress, histological scoring and hepatic CD36 expression. Results: In lean mice, IH elevated serum and hepatic triglyceride and free fatty acid (FFA) levels, in line with upregulation of hepatic CD36 expression and myeloperoxidase (MPO)-positive cells in support of inflammatory infiltrates along with increase in serum malondialdehyde (MDA), C-X-C motif chemokine ligand 1(CXCL-1) and monocyte chemoattractant protein-1 (MCP-1). In diet-induced obese mice, an increase in hepatic alanine transaminase (ALT) activity, serum and hepatic levels of lipid parameters and inflammatory markers, serum MDA level, hepatic expressions of CD36 and α-smooth muscle actin (α-SMA), and MPO-positive cells was observed. IH potentiated hepatic ALT activity, serum CXCL-1 and hepatic interleukin-6 (IL-6), in line with inflammatory infiltrates, but paradoxically, reduced hepatic FFA level and hepatic CD36 expression, compared to obese mice without IH exposure. However, IH further augmented diet-induced liver steatosis and fibrosis as shown by histological scores. Conclusion: This study contributes to support that IH featuring OSA may lead to liver injury via differential regulation of hepatic CD36 expression in lean and diet-induced obese mice.</p> | - |
dc.language | eng | - |
dc.publisher | Elsevier | - |
dc.relation.ispartof | Biomedical Journal | - |
dc.subject | Inflammation | - |
dc.subject | Intermittent hypoxia | - |
dc.subject | Liver injury | - |
dc.subject | Obstructive sleep apnea | - |
dc.subject | Oxidative stress | - |
dc.title | The effects of intermittent hypoxia on hepatic expression of fatty acid translocase CD36 in lean and diet-induced obese mice | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.bj.2022.10.003 | - |
dc.identifier.pmid | 36244649 | - |
dc.identifier.scopus | eid_2-s2.0-85149107343 | - |
dc.identifier.volume | 46 | - |
dc.identifier.issue | 5 | - |
dc.identifier.issnl | 2319-4170 | - |