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- Publisher Website: 10.1007/s00203-024-03918-4
- Scopus: eid_2-s2.0-85187128709
- PMID: 38462558
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Article: D-histidine combated biofilm formation and enhanced the effect of amikacin against Pseudomonas aeruginosa in vitro
Title | D-histidine combated biofilm formation and enhanced the effect of amikacin against Pseudomonas aeruginosa in vitro |
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Authors | |
Keywords | Biofilm D-amino acid D-histidine Pseudomonas aeruginosa Quorum sensing |
Issue Date | 2024 |
Citation | Archives of Microbiology, 2024, v. 206, n. 4, article no. 148 How to Cite? |
Abstract | Pseudomonas aeruginosa is an opportunistic gram-negative pathogenic microorganism that poses a significant challenge in clinical treatment. Antibiotics exhibit limited efficacy against mature biofilm, culminating in an increase in the number of antibiotic-resistant strains. Therefore, novel strategies are essential to enhance the effectiveness of antibiotics against Pseudomonas aeruginosa biofilms. D-histidine has been previously identified as a prospective anti-biofilm agent. However, limited attention has been directed towards its impact on Pseudomonas aeruginosa. Therefore, this study was undertaken to explore the effect of D-histidine on Pseudomonas aeruginosa in vitro. Our results demonstrated that D-histidine downregulated the mRNA expression of virulence and quorum sensing (QS)-associated genes in Pseudomonas aeruginosa PAO1 without affecting bacterial growth. Swarming and swimming motility tests revealed that D-histidine significantly reduced the motility and pathogenicity of PAO1. Moreover, crystal violet staining and confocal laser scanning microscopy demonstrated that D-histidine inhibited biofilm formation and triggered the disassembly of mature biofilms. Notably, D-histidine increased the susceptibility of PAO1 to amikacin compared to that in the amikacin-alone group. These findings underscore the efficacy of D-histidine in combating Pseudomonas aeruginosa by reducing biofilm formation and increasing biofilm disassembly. Moreover, the combination of amikacin and D-histidine induced a synergistic effect against Pseudomonas aeruginosa biofilms, suggesting the potential utility of D-histidine as a preventive strategy against biofilm-associated infections caused by Pseudomonas aeruginosa. |
Persistent Identifier | http://hdl.handle.net/10722/350043 |
ISSN | 2023 Impact Factor: 2.3 2023 SCImago Journal Rankings: 0.589 |
DC Field | Value | Language |
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dc.contributor.author | Zhang, Haichuan | - |
dc.contributor.author | Mi, Zhongwen | - |
dc.contributor.author | Wang, Junmin | - |
dc.contributor.author | Zhang, Jing | - |
dc.date.accessioned | 2024-10-17T07:02:41Z | - |
dc.date.available | 2024-10-17T07:02:41Z | - |
dc.date.issued | 2024 | - |
dc.identifier.citation | Archives of Microbiology, 2024, v. 206, n. 4, article no. 148 | - |
dc.identifier.issn | 0302-8933 | - |
dc.identifier.uri | http://hdl.handle.net/10722/350043 | - |
dc.description.abstract | Pseudomonas aeruginosa is an opportunistic gram-negative pathogenic microorganism that poses a significant challenge in clinical treatment. Antibiotics exhibit limited efficacy against mature biofilm, culminating in an increase in the number of antibiotic-resistant strains. Therefore, novel strategies are essential to enhance the effectiveness of antibiotics against Pseudomonas aeruginosa biofilms. D-histidine has been previously identified as a prospective anti-biofilm agent. However, limited attention has been directed towards its impact on Pseudomonas aeruginosa. Therefore, this study was undertaken to explore the effect of D-histidine on Pseudomonas aeruginosa in vitro. Our results demonstrated that D-histidine downregulated the mRNA expression of virulence and quorum sensing (QS)-associated genes in Pseudomonas aeruginosa PAO1 without affecting bacterial growth. Swarming and swimming motility tests revealed that D-histidine significantly reduced the motility and pathogenicity of PAO1. Moreover, crystal violet staining and confocal laser scanning microscopy demonstrated that D-histidine inhibited biofilm formation and triggered the disassembly of mature biofilms. Notably, D-histidine increased the susceptibility of PAO1 to amikacin compared to that in the amikacin-alone group. These findings underscore the efficacy of D-histidine in combating Pseudomonas aeruginosa by reducing biofilm formation and increasing biofilm disassembly. Moreover, the combination of amikacin and D-histidine induced a synergistic effect against Pseudomonas aeruginosa biofilms, suggesting the potential utility of D-histidine as a preventive strategy against biofilm-associated infections caused by Pseudomonas aeruginosa. | - |
dc.language | eng | - |
dc.relation.ispartof | Archives of Microbiology | - |
dc.subject | Biofilm | - |
dc.subject | D-amino acid | - |
dc.subject | D-histidine | - |
dc.subject | Pseudomonas aeruginosa | - |
dc.subject | Quorum sensing | - |
dc.title | D-histidine combated biofilm formation and enhanced the effect of amikacin against Pseudomonas aeruginosa in vitro | - |
dc.type | Article | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1007/s00203-024-03918-4 | - |
dc.identifier.pmid | 38462558 | - |
dc.identifier.scopus | eid_2-s2.0-85187128709 | - |
dc.identifier.volume | 206 | - |
dc.identifier.issue | 4 | - |
dc.identifier.spage | article no. 148 | - |
dc.identifier.epage | article no. 148 | - |
dc.identifier.eissn | 1432-072X | - |