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- Publisher Website: 10.1073/pnas.2309384121
- Scopus: eid_2-s2.0-85183240534
- PMID: 38252835
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Article: Graphene sandwich–based biological specimen preparation for cryo-EM analysis
Title | Graphene sandwich–based biological specimen preparation for cryo-EM analysis |
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Authors | |
Keywords | beam-induced motion charging effect cryo-EM graphene sandwich sample preparation |
Issue Date | 2024 |
Citation | Proceedings of the National Academy of Sciences of the United States of America, 2024, v. 121, n. 5, article no. e2309384121 How to Cite? |
Abstract | High-quality specimen preparation plays a crucial role in cryo-electron microscopy (cryo-EM) structural analysis. In this study, we have developed a reliable and convenient technique called the graphene sandwich method for preparing cryo-EM specimens. This method involves using two layers of graphene films that enclose macromolecules on both sides, allowing for an appropriate ice thickness for cryo-EM analysis. The graphene sandwich helps to mitigate beam-induced charging effect and reduce particle motion compared to specimens prepared using the traditional method with graphene support on only one side, therefore improving the cryo-EM data quality. These advancements may open new opportunities to expand the use of graphene in the field of biological electron microscopy. |
Persistent Identifier | http://hdl.handle.net/10722/351493 |
ISSN | 2023 Impact Factor: 9.4 2023 SCImago Journal Rankings: 3.737 |
DC Field | Value | Language |
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dc.contributor.author | Xu, Jie | - |
dc.contributor.author | Gao, Xiaoyin | - |
dc.contributor.author | Zheng, Liming | - |
dc.contributor.author | Jia, Xia | - |
dc.contributor.author | Xu, Kui | - |
dc.contributor.author | Ma, Yuwei | - |
dc.contributor.author | Wei, Xiaoding | - |
dc.contributor.author | Liu, Nan | - |
dc.contributor.author | Peng, Hailin | - |
dc.contributor.author | Wang, Hong Wei | - |
dc.date.accessioned | 2024-11-20T03:56:42Z | - |
dc.date.available | 2024-11-20T03:56:42Z | - |
dc.date.issued | 2024 | - |
dc.identifier.citation | Proceedings of the National Academy of Sciences of the United States of America, 2024, v. 121, n. 5, article no. e2309384121 | - |
dc.identifier.issn | 0027-8424 | - |
dc.identifier.uri | http://hdl.handle.net/10722/351493 | - |
dc.description.abstract | High-quality specimen preparation plays a crucial role in cryo-electron microscopy (cryo-EM) structural analysis. In this study, we have developed a reliable and convenient technique called the graphene sandwich method for preparing cryo-EM specimens. This method involves using two layers of graphene films that enclose macromolecules on both sides, allowing for an appropriate ice thickness for cryo-EM analysis. The graphene sandwich helps to mitigate beam-induced charging effect and reduce particle motion compared to specimens prepared using the traditional method with graphene support on only one side, therefore improving the cryo-EM data quality. These advancements may open new opportunities to expand the use of graphene in the field of biological electron microscopy. | - |
dc.language | eng | - |
dc.relation.ispartof | Proceedings of the National Academy of Sciences of the United States of America | - |
dc.subject | beam-induced motion | - |
dc.subject | charging effect | - |
dc.subject | cryo-EM | - |
dc.subject | graphene sandwich | - |
dc.subject | sample preparation | - |
dc.title | Graphene sandwich–based biological specimen preparation for cryo-EM analysis | - |
dc.type | Article | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1073/pnas.2309384121 | - |
dc.identifier.pmid | 38252835 | - |
dc.identifier.scopus | eid_2-s2.0-85183240534 | - |
dc.identifier.volume | 121 | - |
dc.identifier.issue | 5 | - |
dc.identifier.spage | article no. e2309384121 | - |
dc.identifier.epage | article no. e2309384121 | - |
dc.identifier.eissn | 1091-6490 | - |