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Article: Graphene sandwich–based biological specimen preparation for cryo-EM analysis

TitleGraphene sandwich–based biological specimen preparation for cryo-EM analysis
Authors
Keywordsbeam-induced motion
charging effect
cryo-EM
graphene sandwich
sample preparation
Issue Date2024
Citation
Proceedings of the National Academy of Sciences of the United States of America, 2024, v. 121, n. 5, article no. e2309384121 How to Cite?
AbstractHigh-quality specimen preparation plays a crucial role in cryo-electron microscopy (cryo-EM) structural analysis. In this study, we have developed a reliable and convenient technique called the graphene sandwich method for preparing cryo-EM specimens. This method involves using two layers of graphene films that enclose macromolecules on both sides, allowing for an appropriate ice thickness for cryo-EM analysis. The graphene sandwich helps to mitigate beam-induced charging effect and reduce particle motion compared to specimens prepared using the traditional method with graphene support on only one side, therefore improving the cryo-EM data quality. These advancements may open new opportunities to expand the use of graphene in the field of biological electron microscopy.
Persistent Identifierhttp://hdl.handle.net/10722/351493
ISSN
2023 Impact Factor: 9.4
2023 SCImago Journal Rankings: 3.737

 

DC FieldValueLanguage
dc.contributor.authorXu, Jie-
dc.contributor.authorGao, Xiaoyin-
dc.contributor.authorZheng, Liming-
dc.contributor.authorJia, Xia-
dc.contributor.authorXu, Kui-
dc.contributor.authorMa, Yuwei-
dc.contributor.authorWei, Xiaoding-
dc.contributor.authorLiu, Nan-
dc.contributor.authorPeng, Hailin-
dc.contributor.authorWang, Hong Wei-
dc.date.accessioned2024-11-20T03:56:42Z-
dc.date.available2024-11-20T03:56:42Z-
dc.date.issued2024-
dc.identifier.citationProceedings of the National Academy of Sciences of the United States of America, 2024, v. 121, n. 5, article no. e2309384121-
dc.identifier.issn0027-8424-
dc.identifier.urihttp://hdl.handle.net/10722/351493-
dc.description.abstractHigh-quality specimen preparation plays a crucial role in cryo-electron microscopy (cryo-EM) structural analysis. In this study, we have developed a reliable and convenient technique called the graphene sandwich method for preparing cryo-EM specimens. This method involves using two layers of graphene films that enclose macromolecules on both sides, allowing for an appropriate ice thickness for cryo-EM analysis. The graphene sandwich helps to mitigate beam-induced charging effect and reduce particle motion compared to specimens prepared using the traditional method with graphene support on only one side, therefore improving the cryo-EM data quality. These advancements may open new opportunities to expand the use of graphene in the field of biological electron microscopy.-
dc.languageeng-
dc.relation.ispartofProceedings of the National Academy of Sciences of the United States of America-
dc.subjectbeam-induced motion-
dc.subjectcharging effect-
dc.subjectcryo-EM-
dc.subjectgraphene sandwich-
dc.subjectsample preparation-
dc.titleGraphene sandwich–based biological specimen preparation for cryo-EM analysis-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1073/pnas.2309384121-
dc.identifier.pmid38252835-
dc.identifier.scopuseid_2-s2.0-85183240534-
dc.identifier.volume121-
dc.identifier.issue5-
dc.identifier.spagearticle no. e2309384121-
dc.identifier.epagearticle no. e2309384121-
dc.identifier.eissn1091-6490-

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