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Article: Derivation of trophoblast stem cells from human expanded potential stem cells

TitleDerivation of trophoblast stem cells from human expanded potential stem cells
Authors
Xu, ShaoWang, SidongTam, Timothy Theodore Ka KiLiu, PengtaoRuan, Degong
KeywordsCell Biology
Cell Differentiation
Developmental Biology
Stem Cells
Issue Date16-Jun-2023
PublisherCell Press
Citation
STAR Protocols, 2023, v. 4, n. 2 How to Cite?
DOI: http://dx.doi.org/10.1016/j.xpro.2023.102354
Abstract

Human trophoblast development study has long been limited by the lack of suitable materials. Here we present a detailed protocol for the differentiation of human expanded potential stem cells (hEPSCs) into human trophoblast stem cells (TSCs) and for the subsequent establishment of TSC lines. The hEPSC-derived TSC lines can be continuously passaged and are functional in further differentiation into syncytiotrophoblasts and extravillous trophoblasts. The hEPSC-TSC system offers a valuable cell source for studying human trophoblast development in pregnancy. For complete details on the use and execution of this protocol, please refer to Gao et al. (2019) and Ruan et al. (2022).


Persistent Identifierhttp://hdl.handle.net/10722/357486
ISI Accession Number ID
WOS:001058583200001

 

DC FieldValueLanguage
dc.contributor.authorXu, Shao-
dc.contributor.authorWang, Sidong-
dc.contributor.authorTam, Timothy Theodore Ka Ki-
dc.contributor.authorLiu, Pengtao-
dc.contributor.authorRuan, Degong-
dc.date.accessioned2025-07-22T03:13:02Z-
dc.date.available2025-07-22T03:13:02Z-
dc.date.issued2023-06-16-
dc.identifier.citationSTAR Protocols, 2023, v. 4, n. 2-
dc.identifier.urihttp://hdl.handle.net/10722/357486-
dc.description.abstract<p>Human trophoblast development study has long been limited by the lack of suitable materials. Here we present a detailed protocol for the differentiation of human expanded potential stem cells (hEPSCs) into human trophoblast stem cells (TSCs) and for the subsequent establishment of TSC lines. The hEPSC-derived TSC lines can be continuously passaged and are functional in further differentiation into syncytiotrophoblasts and extravillous trophoblasts. The hEPSC-TSC system offers a valuable cell source for studying human trophoblast development in pregnancy. For complete details on the use and execution of this protocol, please refer to Gao et al. (2019) and Ruan et al. (2022).</p>-
dc.languageeng-
dc.publisherCell Press-
dc.relation.ispartofSTAR Protocols-
dc.subjectCell Biology-
dc.subjectCell Differentiation-
dc.subjectDevelopmental Biology-
dc.subjectStem Cells-
dc.titleDerivation of trophoblast stem cells from human expanded potential stem cells-
dc.typeArticle-
dc.identifier.doi10.1016/j.xpro.2023.102354-
dc.identifier.pmid37300826-
dc.identifier.scopuseid_2-s2.0-85161648592-
dc.identifier.volume4-
dc.identifier.issue2-
dc.identifier.eissn2666-1667-
dc.identifier.isiWOS:001058583200001-
dc.identifier.issnl2666-1667-

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