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Article: Oblique-plane single-molecule localization microscopy for tissues and small intact animals

TitleOblique-plane single-molecule localization microscopy for tissues and small intact animals
Authors
Kim, JeongminWojcik, MichalWang, YuanMoon, SeonahZin, Emilia A.Marnani, NadiaNewman, Zachary L.Flannery, John G.Xu, KeZhang, Xiang
Issue Date2019
Citation
Nature Methods, 2019, v. 16, n. 9, p. 853-857 How to Cite?
DOI: http://dx.doi.org/10.1038/s41592-019-0510-z
AbstractSingle-molecule localization microscopy (SMLM), while well established for cultured cells, is not yet fully compatible with tissue-scale samples. We introduce single-molecule oblique-plane microscopy (obSTORM), which by directly imaging oblique sections of samples with oblique light-sheet illumination offers a deep and volumetric SMLM platform that is convenient for standard tissue samples and small intact animals. We demonstrate super-resolution imaging at depths of up to 66 µm for cells, Caenorhabditis elegans gonads, Drosophilamelanogaster larval brain, mouse retina and brain sections, and whole stickleback fish.
Persistent Identifierhttp://hdl.handle.net/10722/369053
ISSN
1548-7091
2023 Impact Factor: 36.1
2023 SCImago Journal Rankings: 14.796

 

DC FieldValueLanguage
dc.contributor.authorKim, Jeongmin-
dc.contributor.authorWojcik, Michal-
dc.contributor.authorWang, Yuan-
dc.contributor.authorMoon, Seonah-
dc.contributor.authorZin, Emilia A.-
dc.contributor.authorMarnani, Nadia-
dc.contributor.authorNewman, Zachary L.-
dc.contributor.authorFlannery, John G.-
dc.contributor.authorXu, Ke-
dc.contributor.authorZhang, Xiang-
dc.date.accessioned2026-01-16T03:15:24Z-
dc.date.available2026-01-16T03:15:24Z-
dc.date.issued2019-
dc.identifier.citationNature Methods, 2019, v. 16, n. 9, p. 853-857-
dc.identifier.issn1548-7091-
dc.identifier.urihttp://hdl.handle.net/10722/369053-
dc.description.abstractSingle-molecule localization microscopy (SMLM), while well established for cultured cells, is not yet fully compatible with tissue-scale samples. We introduce single-molecule oblique-plane microscopy (obSTORM), which by directly imaging oblique sections of samples with oblique light-sheet illumination offers a deep and volumetric SMLM platform that is convenient for standard tissue samples and small intact animals. We demonstrate super-resolution imaging at depths of up to 66 µm for cells, Caenorhabditis elegans gonads, Drosophilamelanogaster larval brain, mouse retina and brain sections, and whole stickleback fish.-
dc.languageeng-
dc.relation.ispartofNature Methods-
dc.titleOblique-plane single-molecule localization microscopy for tissues and small intact animals-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1038/s41592-019-0510-z-
dc.identifier.pmid31427757-
dc.identifier.scopuseid_2-s2.0-85070800992-
dc.identifier.volume16-
dc.identifier.issue9-
dc.identifier.spage853-
dc.identifier.epage857-
dc.identifier.eissn1548-7105-

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