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- Publisher Website: 10.1099/00221287-148-2-507
- Scopus: eid_2-s2.0-0036175114
- PMID: 11832514
- WOS: WOS:000173748500019
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Article: The metIC operon involved in methionine biosynthesis in Bacillus subtilis is controlled by transcription antitermination
Title | The metIC operon involved in methionine biosynthesis in Bacillus subtilis is controlled by transcription antitermination |
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Authors | |
Keywords | Regulation by antitermination Sulfur metabolism |
Issue Date | 2002 |
Publisher | Society for General Microbiology. The Journal's web site is located at http://mic.sgmjournals.org |
Citation | Microbiology, 2002, v. 148 n. pt 2, p. 507-518 How to Cite? |
Abstract | There are two major pathways for methionine biosynthesis in micro-organisms. Little is known about these pathways in Bacillus subtilis. The authors assigned a function to the metI (formerly yjcI) and metC (formerly yjcJ) genes of B. subtilis by complementing Escherichia coli metB and metC mutants, analysing the phenotype of B. subtilis metI and metC mutants, and carrying out enzyme activity assays. These genes encode polypeptides belonging to the cystathionine -synthase family of proteins. Interestingly, the MetI protein has both cystathionine -synthase and O-acetylhomoserine thiolyase activities, whereas the MetC protein is a cystathionine ß-lyase. In B. subtilis, the transsulfuration and the thiolation pathways are functional in vivo. Due to its dual activity, the MetI protein participates in both pathways. The metI and metC genes form an operon, the expression of which is subject to sulfur-dependent regulation. When the sulfur source is sulfate or cysteine the transcription of this operon is high. Conversely, when the sulfur source is methionine its transcription is low. An S-box sequence, which is located upstream of the metI gene, is involved in the regulation of the metIC operon. Northern blot experiments demonstrated the existence of two transcripts: a small transcript corresponding to the premature transcription termination at the terminator present in the S-box and a large one corresponding to transcription of the complete metIC operon. When methionine levels were limiting, the amount of the full-length transcript increased. These results substantiate a model of regulation by transcription antitermination. |
Persistent Identifier | http://hdl.handle.net/10722/42040 |
ISSN | 2023 Impact Factor: 2.6 2023 SCImago Journal Rankings: 0.750 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Auger, S | en_HK |
dc.contributor.author | Yuen, WH | en_HK |
dc.contributor.author | Danchin, A | en_HK |
dc.contributor.author | Martin-Verstraete, I | en_HK |
dc.date.accessioned | 2007-01-08T02:27:33Z | - |
dc.date.available | 2007-01-08T02:27:33Z | - |
dc.date.issued | 2002 | en_HK |
dc.identifier.citation | Microbiology, 2002, v. 148 n. pt 2, p. 507-518 | en_HK |
dc.identifier.issn | 1350-0872 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/42040 | - |
dc.description.abstract | There are two major pathways for methionine biosynthesis in micro-organisms. Little is known about these pathways in Bacillus subtilis. The authors assigned a function to the metI (formerly yjcI) and metC (formerly yjcJ) genes of B. subtilis by complementing Escherichia coli metB and metC mutants, analysing the phenotype of B. subtilis metI and metC mutants, and carrying out enzyme activity assays. These genes encode polypeptides belonging to the cystathionine -synthase family of proteins. Interestingly, the MetI protein has both cystathionine -synthase and O-acetylhomoserine thiolyase activities, whereas the MetC protein is a cystathionine ß-lyase. In B. subtilis, the transsulfuration and the thiolation pathways are functional in vivo. Due to its dual activity, the MetI protein participates in both pathways. The metI and metC genes form an operon, the expression of which is subject to sulfur-dependent regulation. When the sulfur source is sulfate or cysteine the transcription of this operon is high. Conversely, when the sulfur source is methionine its transcription is low. An S-box sequence, which is located upstream of the metI gene, is involved in the regulation of the metIC operon. Northern blot experiments demonstrated the existence of two transcripts: a small transcript corresponding to the premature transcription termination at the terminator present in the S-box and a large one corresponding to transcription of the complete metIC operon. When methionine levels were limiting, the amount of the full-length transcript increased. These results substantiate a model of regulation by transcription antitermination. | en_HK |
dc.format.extent | 336734 bytes | - |
dc.format.extent | 25600 bytes | - |
dc.format.extent | 363115 bytes | - |
dc.format.mimetype | application/pdf | - |
dc.format.mimetype | application/msword | - |
dc.format.mimetype | application/pdf | - |
dc.language | eng | en_HK |
dc.publisher | Society for General Microbiology. The Journal's web site is located at http://mic.sgmjournals.org | en_HK |
dc.subject | Regulation by antitermination | - |
dc.subject | Sulfur metabolism | - |
dc.subject.mesh | Bacillus subtilis - genetics - metabolism | en_HK |
dc.subject.mesh | Methionine - blood | en_HK |
dc.subject.mesh | Multienzyme complexes | en_HK |
dc.subject.mesh | Saccharomyces cerevisiae proteins | en_HK |
dc.subject.mesh | Gene expression regulation, bacterial | en_HK |
dc.title | The metIC operon involved in methionine biosynthesis in Bacillus subtilis is controlled by transcription antitermination | en_HK |
dc.type | Article | en_HK |
dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1350-0872&volume=148&issue=pt 2&spage=507&epage=518&date=2002&atitle=The+metIC+operon+involved+in+methionine+biosynthesis+in+Bacillus+subtilis+is+controlled+by+transcription+antitermination | en_HK |
dc.description.nature | published_or_final_version | en_HK |
dc.identifier.doi | 10.1099/00221287-148-2-507 | - |
dc.identifier.pmid | 11832514 | - |
dc.identifier.scopus | eid_2-s2.0-0036175114 | - |
dc.identifier.hkuros | 69003 | - |
dc.identifier.isi | WOS:000173748500019 | - |
dc.identifier.issnl | 1350-0872 | - |