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Article: Substance P-immunoreactive neurons in hamster retinas
Title | Substance P-immunoreactive neurons in hamster retinas |
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Authors | |
Keywords | Amacrine cells Dorsal lateral geniculate nucleus Optic nerve section Retinal ganglion cells Substance P |
Issue Date | 1999 |
Publisher | Cambridge University Press. The Journal's web site is located at http://journals.cambridge.org/action/displayJournal?jid=VNS |
Citation | Visual Neuroscience, 1999, v. 16 n. 3, p. 475-481 How to Cite? |
Abstract | Light-microscopic immunocytochemistry was utilized to localize the different populations of substance P-immunoreactive (SP-IR) neurons in the hamster retina. Based on observation of 2505 SP-IR neurons in transverse sections, 34% were amacrine cells whose pear-shaped or round cell bodies (7-8 μm) were situated in the inner half of the inner nuclear layer (INL) or in the inner plexiform layer (IPL), while 66% of SP-IR somata (6-20 μm) were located in the ganglion cell layer (GCL) which were interpreted to be displaced amacrine cells and retinal ganglion cells (RGCs). At least three types of SP-IR amacrine cells were identified. The SP-IR processes were distributed in strata 1, 3, and 5 with the densest plexus in stratum 5 of the inner plexiform layer. In the wholemounted retina, the SP-IR cells were found to be distributed throughout the entire retina and their mean number was estimated to be 4224 ± 76. Two experiments were performed to clarify whether any of the SP-IR neurons in the GCL were RGCs. The first experiment demonstrated the presence of SP-IR RGCs by retrogradely labeling the RGCs and subsequently staining the SP-IR cells in the retina using immunocytochemistry. The second experiment identified SP-IR central projections of RGCs to the contralateral dorsal lateral geniculate nucleus. This projection disappeared following removal of the contralateral eye. The number of SP-IR RGCs was estimated following optic nerve section. At 2 months after sectioning the optic nerve, the total number of SP-IR neurons in the GCL reduced from 4224 ± 76 to a mean of 1192 ± 139. Assuming that all SP-IR neurons in the GCL which disappeared after nerve section were RGCs, the number of SP-IR RGCs was estimated to be 3032, representing 3-4% of the total RGCs. In summary, findings of the present study provide evidence for the existence of SP-IR RGCs in the hamster retina. |
Persistent Identifier | http://hdl.handle.net/10722/42334 |
ISSN | 2023 Impact Factor: 1.1 2023 SCImago Journal Rankings: 0.472 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Li, HB | en_HK |
dc.contributor.author | So, KF | en_HK |
dc.contributor.author | Cheuk, W | en_HK |
dc.date.accessioned | 2007-01-29T08:47:08Z | - |
dc.date.available | 2007-01-29T08:47:08Z | - |
dc.date.issued | 1999 | en_HK |
dc.identifier.citation | Visual Neuroscience, 1999, v. 16 n. 3, p. 475-481 | en_HK |
dc.identifier.issn | 0952-5238 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/42334 | - |
dc.description.abstract | Light-microscopic immunocytochemistry was utilized to localize the different populations of substance P-immunoreactive (SP-IR) neurons in the hamster retina. Based on observation of 2505 SP-IR neurons in transverse sections, 34% were amacrine cells whose pear-shaped or round cell bodies (7-8 μm) were situated in the inner half of the inner nuclear layer (INL) or in the inner plexiform layer (IPL), while 66% of SP-IR somata (6-20 μm) were located in the ganglion cell layer (GCL) which were interpreted to be displaced amacrine cells and retinal ganglion cells (RGCs). At least three types of SP-IR amacrine cells were identified. The SP-IR processes were distributed in strata 1, 3, and 5 with the densest plexus in stratum 5 of the inner plexiform layer. In the wholemounted retina, the SP-IR cells were found to be distributed throughout the entire retina and their mean number was estimated to be 4224 ± 76. Two experiments were performed to clarify whether any of the SP-IR neurons in the GCL were RGCs. The first experiment demonstrated the presence of SP-IR RGCs by retrogradely labeling the RGCs and subsequently staining the SP-IR cells in the retina using immunocytochemistry. The second experiment identified SP-IR central projections of RGCs to the contralateral dorsal lateral geniculate nucleus. This projection disappeared following removal of the contralateral eye. The number of SP-IR RGCs was estimated following optic nerve section. At 2 months after sectioning the optic nerve, the total number of SP-IR neurons in the GCL reduced from 4224 ± 76 to a mean of 1192 ± 139. Assuming that all SP-IR neurons in the GCL which disappeared after nerve section were RGCs, the number of SP-IR RGCs was estimated to be 3032, representing 3-4% of the total RGCs. In summary, findings of the present study provide evidence for the existence of SP-IR RGCs in the hamster retina. | en_HK |
dc.format.extent | 978680 bytes | - |
dc.format.extent | 25600 bytes | - |
dc.format.mimetype | application/pdf | - |
dc.format.mimetype | application/msword | - |
dc.language | eng | en_HK |
dc.publisher | Cambridge University Press. The Journal's web site is located at http://journals.cambridge.org/action/displayJournal?jid=VNS | en_HK |
dc.relation.ispartof | Visual Neuroscience | en_HK |
dc.rights | Visual Neuroscience. Copyright © Cambridge University Press. | en_HK |
dc.subject | Amacrine cells | en_HK |
dc.subject | Dorsal lateral geniculate nucleus | en_HK |
dc.subject | Optic nerve section | en_HK |
dc.subject | Retinal ganglion cells | en_HK |
dc.subject | Substance P | en_HK |
dc.subject.mesh | Immunohistochemistry | en_HK |
dc.subject.mesh | Neurons - chemistry | en_HK |
dc.subject.mesh | Retina - chemistry - cytology | en_HK |
dc.subject.mesh | Retinal ganglion cells - chemistry | en_HK |
dc.subject.mesh | Substance p - analysis | en_HK |
dc.title | Substance P-immunoreactive neurons in hamster retinas | en_HK |
dc.type | Article | en_HK |
dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0952-5238&volume=16&issue=3&spage=475&epage=481&date=1999&atitle=Substance+P-immunoreactive+neurons+in+hamster+retinas | en_HK |
dc.identifier.email | So, KF:hrmaskf@hkucc.hku.hk | en_HK |
dc.identifier.authority | So, KF=rp00329 | en_HK |
dc.description.nature | published_or_final_version | en_HK |
dc.identifier.doi | 10.1017/S0952523899163089 | en_HK |
dc.identifier.pmid | 10349968 | - |
dc.identifier.scopus | eid_2-s2.0-0033136353 | en_HK |
dc.identifier.hkuros | 40883 | - |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0033136353&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 16 | en_HK |
dc.identifier.issue | 3 | en_HK |
dc.identifier.spage | 475 | en_HK |
dc.identifier.epage | 481 | en_HK |
dc.identifier.isi | WOS:000080234000008 | - |
dc.publisher.place | United Kingdom | en_HK |
dc.identifier.scopusauthorid | Li, HB=13008179800 | en_HK |
dc.identifier.scopusauthorid | So, KF=34668391300 | en_HK |
dc.identifier.scopusauthorid | Cheuk, W=7003958433 | en_HK |
dc.identifier.issnl | 0952-5238 | - |