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- Publisher Website: 10.1136/gut.2005.077776
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Article: Eradication of Helicobacter pylori infection reverses E-cadherin promoter hypermethylation
Title | Eradication of Helicobacter pylori infection reverses E-cadherin promoter hypermethylation |
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Authors | |
Issue Date | 2006 |
Publisher | BMJ Publishing Group. The Journal's web site is located at http://gut.bmjjournals.com/ |
Citation | Gut, 2006, v. 55 n. 4, p. 463-468 How to Cite? |
Abstract | Background: E-cadherin methylation is important in gastric carcinogenesis. Reversing hypermethylation may halt the carcinogenic process. We have previously reported that Helicobacter pylori infection is associated with E-cadherin methylation in chronic gastritis patients. Aim: To examine if eradication of H pylori could reverse E-cadherin methylation. Methods: Patients with dyspepsia and positive for H pylori infection, with a mucosal biopsy showing chronic active gastritis, were randomised to receive H pylori eradication therapy (group 1, n = 41) or no treatment (group 2, n = 40), and were followed up prospectively. Gastric mucosae were taken for methylation assay at week 0 (before treatment) and week 6 (after treatment). Archived specimens of intestinal metaplasia with H pylori infection (n = 22) and without (n = 19) were retrieved for methylation analysis. Methylation was assessed using methylation specific polymerase chain reaction and sequencing. Results: Methylation at E-cadherin was detected in 46% (19/41) and 17% (7/41) of patients at weeks 0 and 6, respectively, in group 1 (p = 0.004); 78.9% (15/19) of specimens were unmethylated after eradication of H pylori. Mucosal biopsy showed chronic inactive gastritis in 35 patients, intestinal metaplasia in one, and normal mucosa in five at week 6. Methylation was detected in 47.5% (19/40) and 52.5% (21/40) of patients at weeks 0 and 6, respectively, in group 2 (P = 0.5). Gastric mucosal biopsy showed persistent chronic active gastritis in all cases. Methylation frequency did not differ in H pylori positive or negative intestinal metaplastic specimens (72.7% v 63%; p = 0.5). Conclusion: H pylori eradication therapy could reverse methylation in patients with chronic gastritis. This demonstrates an environmental effect on methylation. |
Persistent Identifier | http://hdl.handle.net/10722/44964 |
ISSN | 2023 Impact Factor: 23.0 2023 SCImago Journal Rankings: 8.052 |
PubMed Central ID | |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
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dc.contributor.author | Chan, AOO | en_HK |
dc.contributor.author | Peng, JZ | en_HK |
dc.contributor.author | Lam, SK | en_HK |
dc.contributor.author | Lai, KC | en_HK |
dc.contributor.author | Yuen, MF | en_HK |
dc.contributor.author | Cheung, HKL | en_HK |
dc.contributor.author | Kwong, YL | en_HK |
dc.contributor.author | Rashid, A | en_HK |
dc.contributor.author | Chan, CK | en_HK |
dc.contributor.author | Wong, BCY | en_HK |
dc.date.accessioned | 2007-10-30T06:14:33Z | - |
dc.date.available | 2007-10-30T06:14:33Z | - |
dc.date.issued | 2006 | en_HK |
dc.identifier.citation | Gut, 2006, v. 55 n. 4, p. 463-468 | en_HK |
dc.identifier.issn | 0017-5749 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/44964 | - |
dc.description.abstract | Background: E-cadherin methylation is important in gastric carcinogenesis. Reversing hypermethylation may halt the carcinogenic process. We have previously reported that Helicobacter pylori infection is associated with E-cadherin methylation in chronic gastritis patients. Aim: To examine if eradication of H pylori could reverse E-cadherin methylation. Methods: Patients with dyspepsia and positive for H pylori infection, with a mucosal biopsy showing chronic active gastritis, were randomised to receive H pylori eradication therapy (group 1, n = 41) or no treatment (group 2, n = 40), and were followed up prospectively. Gastric mucosae were taken for methylation assay at week 0 (before treatment) and week 6 (after treatment). Archived specimens of intestinal metaplasia with H pylori infection (n = 22) and without (n = 19) were retrieved for methylation analysis. Methylation was assessed using methylation specific polymerase chain reaction and sequencing. Results: Methylation at E-cadherin was detected in 46% (19/41) and 17% (7/41) of patients at weeks 0 and 6, respectively, in group 1 (p = 0.004); 78.9% (15/19) of specimens were unmethylated after eradication of H pylori. Mucosal biopsy showed chronic inactive gastritis in 35 patients, intestinal metaplasia in one, and normal mucosa in five at week 6. Methylation was detected in 47.5% (19/40) and 52.5% (21/40) of patients at weeks 0 and 6, respectively, in group 2 (P = 0.5). Gastric mucosal biopsy showed persistent chronic active gastritis in all cases. Methylation frequency did not differ in H pylori positive or negative intestinal metaplastic specimens (72.7% v 63%; p = 0.5). Conclusion: H pylori eradication therapy could reverse methylation in patients with chronic gastritis. This demonstrates an environmental effect on methylation. | en_HK |
dc.format.extent | 1048306 bytes | - |
dc.format.extent | 125545 bytes | - |
dc.format.extent | 9342 bytes | - |
dc.format.extent | 2057 bytes | - |
dc.format.mimetype | application/pdf | - |
dc.format.mimetype | application/pdf | - |
dc.format.mimetype | text/plain | - |
dc.format.mimetype | text/plain | - |
dc.language | eng | en_HK |
dc.publisher | BMJ Publishing Group. The Journal's web site is located at http://gut.bmjjournals.com/ | en_HK |
dc.relation.ispartof | Gut | en_HK |
dc.rights | Gut. Copyright © B M J Publishing Group. | en_HK |
dc.subject.mesh | Cadherins-genetics | en_HK |
dc.subject.mesh | Gastritis-genetics | en_HK |
dc.subject.mesh | Helicobacter-Infections-genetics | en_HK |
dc.subject.mesh | Helicobacter-pylori | en_HK |
dc.subject.mesh | Promoter-Regions-Genetics-genetics | en_HK |
dc.title | Eradication of Helicobacter pylori infection reverses E-cadherin promoter hypermethylation | en_HK |
dc.type | Article | en_HK |
dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0017-5749&volume=55&issue=4&spage=463&epage=468&date=2006&atitle=Eradication+of+Helicobacter+pylori+infection+reverses+E-cadherin+promoter+hypermethylation | en_HK |
dc.identifier.email | Yuen, MF:mfyuen@hkucc.hku.hk | en_HK |
dc.identifier.email | Kwong, YL:ylkwong@hku.hk | en_HK |
dc.identifier.email | Wong, BCY:bcywong@hku.hk | en_HK |
dc.identifier.authority | Yuen, MF=rp00479 | en_HK |
dc.identifier.authority | Kwong, YL=rp00358 | en_HK |
dc.identifier.authority | Wong, BCY=rp00429 | en_HK |
dc.description.nature | published_or_final_version | en_HK |
dc.identifier.doi | 10.1136/gut.2005.077776 | en_HK |
dc.identifier.pmid | 16428266 | - |
dc.identifier.pmcid | PMC1856151 | - |
dc.identifier.scopus | eid_2-s2.0-33645119366 | en_HK |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-33645119366&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 55 | en_HK |
dc.identifier.issue | 4 | en_HK |
dc.identifier.spage | 463 | en_HK |
dc.identifier.epage | 468 | en_HK |
dc.identifier.isi | WOS:000236561900012 | - |
dc.publisher.place | United Kingdom | en_HK |
dc.identifier.scopusauthorid | Chan, AOO=7403167965 | en_HK |
dc.identifier.scopusauthorid | Peng, JZ=12796973500 | en_HK |
dc.identifier.scopusauthorid | Lam, SK=7402279473 | en_HK |
dc.identifier.scopusauthorid | Lai, KC=7402135595 | en_HK |
dc.identifier.scopusauthorid | Yuen, MF=7102031955 | en_HK |
dc.identifier.scopusauthorid | Cheung, HKL=7201839335 | en_HK |
dc.identifier.scopusauthorid | Kwong, YL=7102818954 | en_HK |
dc.identifier.scopusauthorid | Rashid, A=7102299914 | en_HK |
dc.identifier.scopusauthorid | Chan, CK=7404813824 | en_HK |
dc.identifier.scopusauthorid | Wong, BCY=7402023340 | en_HK |
dc.identifier.issnl | 0017-5749 | - |