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- Publisher Website: 10.1182/blood-2004-10-4166
- Scopus: eid_2-s2.0-27144497805
- PMID: 15860669
- WOS: WOS:000232134700031
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Article: Chemokine up-regulation in SARS-coronavirus-infected, monocyte-derived human dendritic cells
Title | Chemokine up-regulation in SARS-coronavirus-infected, monocyte-derived human dendritic cells |
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Authors | |
Issue Date | 2005 |
Publisher | American Society of Hematology. The Journal's web site is located at http://bloodjournal.hematologylibrary.org/ |
Citation | Blood, 2005, v. 106 n. 7, p. 2366-2374 How to Cite? |
Abstract | Lymphopenia and increasing viral load in the first 10 days of severe acute respiratory syndrome (SARS) suggested immune evasion by SARS-coronavirus (CoV). In this study, we focused on dendritic cells (DCs) which play important roles in linking the innate and adaptive immunity. SARS-CoV was shown to infect both immature and mature human monocyte-derived DCs by electron microscopy and immunofluorescence. The detection of negative strands of SARS-CoV RNA in DCs suggested viral replication. However, no increase in viral RNA was observed. Using cytopathic assays, no increase in virus titer was detected in infected DCs and cell-culture supernatant, confirming that virus replication was incomplete. No induction of apoptosis or maturation was detected in SARS-CoV-infected DCs. The SARS-CoV-infected DCs showed low expression of antiviral cytokines (interferon α [IFN-α], IFN-β, IFN-γ, and interleukin 12p40 [IL-12p40]), moderate up-regulation of proinflammatory cytokines (tumor necrosis factor α [TNF-α] and IL-6) but significant up-regulation of inflammatory chemokines (macrophage inflammatory protein 1α [MIP-1α], regulated on activation normal T cell expressed and secreted [RANTES]), interferon-inducible protein of 10 kDa [IP-10], and monocyte chemoattractant protein 1 [MCP-1]). The lack of antiviral cytokine response against a background of intense chemokine upregulation could represent a mechanism of immune evasion by SARS-CoV. © 2005 by The American Society of Hematology. |
Persistent Identifier | http://hdl.handle.net/10722/48636 |
ISSN | 2023 Impact Factor: 21.0 2023 SCImago Journal Rankings: 5.272 |
PubMed Central ID | |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Law, HKW | en_HK |
dc.contributor.author | Chung, YC | en_HK |
dc.contributor.author | Hoi, YN | en_HK |
dc.contributor.author | Sin, FS | en_HK |
dc.contributor.author | Yuk, OC | en_HK |
dc.contributor.author | Luk, W | en_HK |
dc.contributor.author | Nicholls, JM | en_HK |
dc.contributor.author | Peiris, JSM | en_HK |
dc.contributor.author | Lau, YL | en_HK |
dc.date.accessioned | 2008-05-22T04:19:41Z | - |
dc.date.available | 2008-05-22T04:19:41Z | - |
dc.date.issued | 2005 | en_HK |
dc.identifier.citation | Blood, 2005, v. 106 n. 7, p. 2366-2374 | en_HK |
dc.identifier.issn | 0006-4971 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/48636 | - |
dc.description.abstract | Lymphopenia and increasing viral load in the first 10 days of severe acute respiratory syndrome (SARS) suggested immune evasion by SARS-coronavirus (CoV). In this study, we focused on dendritic cells (DCs) which play important roles in linking the innate and adaptive immunity. SARS-CoV was shown to infect both immature and mature human monocyte-derived DCs by electron microscopy and immunofluorescence. The detection of negative strands of SARS-CoV RNA in DCs suggested viral replication. However, no increase in viral RNA was observed. Using cytopathic assays, no increase in virus titer was detected in infected DCs and cell-culture supernatant, confirming that virus replication was incomplete. No induction of apoptosis or maturation was detected in SARS-CoV-infected DCs. The SARS-CoV-infected DCs showed low expression of antiviral cytokines (interferon α [IFN-α], IFN-β, IFN-γ, and interleukin 12p40 [IL-12p40]), moderate up-regulation of proinflammatory cytokines (tumor necrosis factor α [TNF-α] and IL-6) but significant up-regulation of inflammatory chemokines (macrophage inflammatory protein 1α [MIP-1α], regulated on activation normal T cell expressed and secreted [RANTES]), interferon-inducible protein of 10 kDa [IP-10], and monocyte chemoattractant protein 1 [MCP-1]). The lack of antiviral cytokine response against a background of intense chemokine upregulation could represent a mechanism of immune evasion by SARS-CoV. © 2005 by The American Society of Hematology. | en_HK |
dc.format.extent | 1048366 bytes | - |
dc.format.extent | 4956 bytes | - |
dc.format.mimetype | application/pdf | - |
dc.format.mimetype | text/plain | - |
dc.language | eng | en_HK |
dc.publisher | American Society of Hematology. The Journal's web site is located at http://bloodjournal.hematologylibrary.org/ | en_HK |
dc.relation.ispartof | Blood | en_HK |
dc.subject.mesh | Interleukin-12 - biosynthesis - metabolism | en_HK |
dc.subject.mesh | Leukocytes, Mononuclear - cytology | en_HK |
dc.subject.mesh | Up-Regulation | en_HK |
dc.subject.mesh | SARS Virus - genetics - metabolism | en_HK |
dc.subject.mesh | Monocytes - cytology | en_HK |
dc.title | Chemokine up-regulation in SARS-coronavirus-infected, monocyte-derived human dendritic cells | en_HK |
dc.type | Article | en_HK |
dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0006-4971&volume=106&issue=7&spage=2366&epage=2374&date=2005&atitle=Chemokine+up-regulation+in+SARS-coronavirus-infected,+monocyte-derived+human+dendritic+cells | en_HK |
dc.identifier.email | Chung, YC: chungey@hkucc.hku.hk | en_HK |
dc.identifier.email | Nicholls, JM: jmnichol@hkucc.hku.hk | en_HK |
dc.identifier.email | Peiris, JSM: malik@hkucc.hku.hk | en_HK |
dc.identifier.email | Lau, YL: lauylung@hku.hk | en_HK |
dc.identifier.authority | Chung, YC=rp00404 | en_HK |
dc.identifier.authority | Nicholls, JM=rp00364 | en_HK |
dc.identifier.authority | Peiris, JSM=rp00410 | en_HK |
dc.identifier.authority | Lau, YL=rp00361 | en_HK |
dc.description.nature | postprint | en_HK |
dc.identifier.doi | 10.1182/blood-2004-10-4166 | en_HK |
dc.identifier.pmid | 15860669 | - |
dc.identifier.pmcid | PMC1895271 | - |
dc.identifier.scopus | eid_2-s2.0-27144497805 | en_HK |
dc.identifier.hkuros | 103548 | - |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-27144497805&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 106 | en_HK |
dc.identifier.issue | 7 | en_HK |
dc.identifier.spage | 2366 | en_HK |
dc.identifier.epage | 2374 | en_HK |
dc.identifier.isi | WOS:000232134700031 | - |
dc.publisher.place | United States | en_HK |
dc.identifier.scopusauthorid | Law, HKW=7101939394 | en_HK |
dc.identifier.scopusauthorid | Chung, YC=7202061836 | en_HK |
dc.identifier.scopusauthorid | Hoi, YN=8968426900 | en_HK |
dc.identifier.scopusauthorid | Sin, FS=8968427000 | en_HK |
dc.identifier.scopusauthorid | Yuk, OC=8968427100 | en_HK |
dc.identifier.scopusauthorid | Luk, W=7005237837 | en_HK |
dc.identifier.scopusauthorid | Nicholls, JM=7201463077 | en_HK |
dc.identifier.scopusauthorid | Peiris, JSM=7005486823 | en_HK |
dc.identifier.scopusauthorid | Lau, YL=7201403380 | en_HK |
dc.identifier.issnl | 0006-4971 | - |