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- Publisher Website: 10.1046/j.1432-1033.2002.03092.x
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- PMID: 12153576
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Article: The proximal cis-acting elements Sp1, Sp3 and E2F regulate mouse mer gene transcription in sertoli cells
Title | The proximal cis-acting elements Sp1, Sp3 and E2F regulate mouse mer gene transcription in sertoli cells |
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Authors | |
Keywords | E2F Mer gene Receptor tyrosine kinases Sertoli cells Sp1 and Sp3 |
Issue Date | 2002 |
Publisher | Blackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/EJB |
Citation | European Journal Of Biochemistry, 2002, v. 269 n. 15, p. 3789-3800 How to Cite? |
Abstract | Mer belongs to the Tyro 3 family of receptor tyrosine kinases (RTKs). Together with Axl and Rse, the three RTKs are believed to play important functional roles in the male gonads because gene knockout male mice lacking all of these receptors are infertile. In the present study, postnatal expression of Axl and Rse in mouse testes decreased during maturation while expression of Mer increased age-dependently during testicular development. To investigate the transcriptional regulation of gene expression in the testis, a ≈ 1.5 kb fragment of the 5′ flanking sequence of Mer was isolated. The sequence lacks a typical TATA or CAAT box. 5′ RACE revealed that the putative major transcriptional start site of Mer is located at +102 bp upstream of the translation initiation site. Using transient transfections of luciferase reporter constructs driven by various lengths of the 5′ flanking sequence, the gene segment -321/+126 showed the highest transcriptional activity in a mouse Sertoli cell line (TM4). DNAase I footprinting experiments revealed four footprints within the region from -321 to -26, including three binding sites for the transcriptional factor Specificity protein 1 (Sp1) and one for an unknown transcriptional factor. Electrophoretic mobility shift assay (EMSA), supershift assay, mutation studies and cotransfection demonstrated that those Sp1 cis-acting motifs interacted either with Sp1 or Sp1/Sp3, depending on location and the nearby nucleotide sequences. An E2F binding site which down-regulates Mer transcription, as revealed by EMSA, deletion and mutation studies, was identified downstream in the proximity of the promoter. Taking all of these data together, the study has demonstrated that Sp1, Sp3, E2F and probably another unknown transcriptional factor play a critical role in regulating the proximal promoter activities of Mer. |
Persistent Identifier | http://hdl.handle.net/10722/48686 |
ISSN | |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Wong, CCS | en_HK |
dc.contributor.author | Lee, WM | en_HK |
dc.date.accessioned | 2008-05-22T04:21:22Z | - |
dc.date.available | 2008-05-22T04:21:22Z | - |
dc.date.issued | 2002 | en_HK |
dc.identifier.citation | European Journal Of Biochemistry, 2002, v. 269 n. 15, p. 3789-3800 | en_HK |
dc.identifier.issn | 0014-2956 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/48686 | - |
dc.description.abstract | Mer belongs to the Tyro 3 family of receptor tyrosine kinases (RTKs). Together with Axl and Rse, the three RTKs are believed to play important functional roles in the male gonads because gene knockout male mice lacking all of these receptors are infertile. In the present study, postnatal expression of Axl and Rse in mouse testes decreased during maturation while expression of Mer increased age-dependently during testicular development. To investigate the transcriptional regulation of gene expression in the testis, a ≈ 1.5 kb fragment of the 5′ flanking sequence of Mer was isolated. The sequence lacks a typical TATA or CAAT box. 5′ RACE revealed that the putative major transcriptional start site of Mer is located at +102 bp upstream of the translation initiation site. Using transient transfections of luciferase reporter constructs driven by various lengths of the 5′ flanking sequence, the gene segment -321/+126 showed the highest transcriptional activity in a mouse Sertoli cell line (TM4). DNAase I footprinting experiments revealed four footprints within the region from -321 to -26, including three binding sites for the transcriptional factor Specificity protein 1 (Sp1) and one for an unknown transcriptional factor. Electrophoretic mobility shift assay (EMSA), supershift assay, mutation studies and cotransfection demonstrated that those Sp1 cis-acting motifs interacted either with Sp1 or Sp1/Sp3, depending on location and the nearby nucleotide sequences. An E2F binding site which down-regulates Mer transcription, as revealed by EMSA, deletion and mutation studies, was identified downstream in the proximity of the promoter. Taking all of these data together, the study has demonstrated that Sp1, Sp3, E2F and probably another unknown transcriptional factor play a critical role in regulating the proximal promoter activities of Mer. | en_HK |
dc.format.extent | 456154 bytes | - |
dc.format.extent | 2457 bytes | - |
dc.format.mimetype | application/pdf | - |
dc.format.mimetype | text/plain | - |
dc.language | eng | en_HK |
dc.publisher | Blackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/EJB | en_HK |
dc.relation.ispartof | European Journal of Biochemistry | en_HK |
dc.rights | European Journal of Biochemistry. Copyright © Blackwell Publishing Ltd. | en_HK |
dc.rights | The definitive version is available at www.blackwell-synergy.com | en_HK |
dc.subject | E2F | en_HK |
dc.subject | Mer gene | en_HK |
dc.subject | Receptor tyrosine kinases | en_HK |
dc.subject | Sertoli cells | en_HK |
dc.subject | Sp1 and Sp3 | en_HK |
dc.title | The proximal cis-acting elements Sp1, Sp3 and E2F regulate mouse mer gene transcription in sertoli cells | en_HK |
dc.type | Article | en_HK |
dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0014-2956&volume=269&issue=15&spage=3789&epage=3800&date=2002&atitle=The+proximal+cis-acting+elements+Sp1,+Sp3+and+E2F+regulate+mouse+mer+gene+transcription+in+Sertoli+cells | en_HK |
dc.identifier.email | Lee, WM: hrszlwm@hku.hk | en_HK |
dc.identifier.authority | Lee, WM=rp00728 | en_HK |
dc.description.nature | postprint | en_HK |
dc.identifier.doi | 10.1046/j.1432-1033.2002.03092.x | en_HK |
dc.identifier.pmid | 12153576 | en_HK |
dc.identifier.scopus | eid_2-s2.0-0036370524 | en_HK |
dc.identifier.hkuros | 69752 | - |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0036370524&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 269 | en_HK |
dc.identifier.issue | 15 | en_HK |
dc.identifier.spage | 3789 | en_HK |
dc.identifier.epage | 3800 | en_HK |
dc.identifier.isi | WOS:000177782100020 | - |
dc.publisher.place | United Kingdom | en_HK |
dc.identifier.scopusauthorid | Wong, CCS=19037067100 | en_HK |
dc.identifier.scopusauthorid | Lee, WM=24799156600 | en_HK |
dc.identifier.issnl | 0014-2956 | - |