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- Publisher Website: 10.1099/00222615-48-8-721
- Scopus: eid_2-s2.0-0032878759
- PMID: 10450995
- WOS: WOS:000081722100003
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Article: Inhibition of growth and secreted aspartyl proteinase production in Candida albicans by lysozyme
Title | Inhibition of growth and secreted aspartyl proteinase production in Candida albicans by lysozyme |
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Authors | |
Issue Date | 1999 |
Publisher | Society for General Microbiology. The Journal's web site is located at http://jmm.sgmjournals.org |
Citation | Journal of Medical Microbiology, 1999, v. 48 n. 8, p. 721-730 How to Cite? |
Abstract | Lysozyme (muramidase) is a non-specific, antimicrobial protein ubiquitous in human mucosal secretions such as saliva. Although its antibacterial and antifungal activities are well recognised, there are no data on the specific concentrations necessary to affect the growth of Candida albicans or about the effect of lysozyme on the production of secreted aspartyl proteinase (Sap), a putative virulence factor of C. albicans. Five Sap-producing isolates of C. albicans were cultured in YCB-BSA medium with various concentrations of lysozyme to examine its effect on yeast cell growth, ultrastructural cellular topography and extracellular and intracellular Sap concentration and activity. Lysozyme was candidacidal at high concentrations and decreased significantly the extracellular Sap concentration at sublethal doses, accompanied by intracellular accumulation of the enzyme. At low concentrations of lysozyme (c. 10 μg/ml). Sap activity decreased more than two-fold and Sap concentration decreased five-fold without any appreciable effect on cell growth or viability. Ultrastructural investigations showed ballooned cells and cells with invaginations (expecially present near bud scars), indicating that cell-wall components may be possible targets for this enzyme. All concentrations of lysozyme tested were well within physiologically attainable levels. These data suggest that lysozyme has, at least, a bimodal action on C. albicans, killing the organism at higher concentrations and modulating Sap metabolism at lower concentrations. |
Persistent Identifier | http://hdl.handle.net/10722/55439 |
ISSN | 2023 Impact Factor: 2.4 2023 SCImago Journal Rankings: 0.752 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Wu, T | en_HK |
dc.contributor.author | Samaranayake, LP | en_HK |
dc.contributor.author | Leung, WK | en_HK |
dc.contributor.author | Sullivan, PA | en_HK |
dc.date.accessioned | 2009-08-06T03:37:08Z | - |
dc.date.available | 2009-08-06T03:37:08Z | - |
dc.date.issued | 1999 | en_HK |
dc.identifier.citation | Journal of Medical Microbiology, 1999, v. 48 n. 8, p. 721-730 | en_HK |
dc.identifier.issn | 0022-2615 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/55439 | - |
dc.description.abstract | Lysozyme (muramidase) is a non-specific, antimicrobial protein ubiquitous in human mucosal secretions such as saliva. Although its antibacterial and antifungal activities are well recognised, there are no data on the specific concentrations necessary to affect the growth of Candida albicans or about the effect of lysozyme on the production of secreted aspartyl proteinase (Sap), a putative virulence factor of C. albicans. Five Sap-producing isolates of C. albicans were cultured in YCB-BSA medium with various concentrations of lysozyme to examine its effect on yeast cell growth, ultrastructural cellular topography and extracellular and intracellular Sap concentration and activity. Lysozyme was candidacidal at high concentrations and decreased significantly the extracellular Sap concentration at sublethal doses, accompanied by intracellular accumulation of the enzyme. At low concentrations of lysozyme (c. 10 μg/ml). Sap activity decreased more than two-fold and Sap concentration decreased five-fold without any appreciable effect on cell growth or viability. Ultrastructural investigations showed ballooned cells and cells with invaginations (expecially present near bud scars), indicating that cell-wall components may be possible targets for this enzyme. All concentrations of lysozyme tested were well within physiologically attainable levels. These data suggest that lysozyme has, at least, a bimodal action on C. albicans, killing the organism at higher concentrations and modulating Sap metabolism at lower concentrations. | en_HK |
dc.language | eng | en_HK |
dc.publisher | Society for General Microbiology. The Journal's web site is located at http://jmm.sgmjournals.org | en_HK |
dc.relation.ispartof | Journal of Medical Microbiology | en_HK |
dc.subject.mesh | Aspartic Endopeptidases - antagonists & inhibitors - metabolism - secretion | en_HK |
dc.subject.mesh | Candida albicans - cytology - enzymology - ultrastructure | en_HK |
dc.subject.mesh | Muramidase - metabolism - pharmacology | en_HK |
dc.subject.mesh | Anti-Infective Agents - pharmacology | en_HK |
dc.subject.mesh | Extracellular Space - drug effects - enzymology | en_HK |
dc.title | Inhibition of growth and secreted aspartyl proteinase production in Candida albicans by lysozyme | en_HK |
dc.type | Article | en_HK |
dc.identifier.email | Samaranayake, LP:lakshman@hku.hk | en_HK |
dc.identifier.email | Leung, WK:ewkleung@hkucc.hku.hk | en_HK |
dc.identifier.authority | Samaranayake, LP=rp00023 | en_HK |
dc.identifier.authority | Leung, WK=rp00019 | en_HK |
dc.description.nature | link_to_OA_fulltext | en_HK |
dc.identifier.doi | 10.1099/00222615-48-8-721 | - |
dc.identifier.pmid | 10450995 | - |
dc.identifier.scopus | eid_2-s2.0-0032878759 | en_HK |
dc.identifier.hkuros | 41782 | - |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0032878759&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 48 | en_HK |
dc.identifier.issue | 8 | en_HK |
dc.identifier.spage | 721 | en_HK |
dc.identifier.epage | 730 | en_HK |
dc.identifier.isi | WOS:000081722100003 | - |
dc.publisher.place | United Kingdom | en_HK |
dc.identifier.scopusauthorid | Wu, T=9237315300 | en_HK |
dc.identifier.scopusauthorid | Samaranayake, LP=7102761002 | en_HK |
dc.identifier.scopusauthorid | Leung, WK=25224691800 | en_HK |
dc.identifier.scopusauthorid | Sullivan, PA=7402033666 | en_HK |
dc.identifier.issnl | 0022-2615 | - |