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Article: Pathway of phytate dephosphorylation by β-propeller phytases of different origins
Title | Pathway of phytate dephosphorylation by β-propeller phytases of different origins |
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Authors | |
Keywords | β-propeller phytase Bacillus spp. myo-inositol phosphate isomers Phytate degradation Shewanella oneidensis |
Issue Date | 2007 |
Publisher | N R C Research Press. The Journal's web site is located at http://pubs.nrc-cnrc.gc.ca/cgi-bin/rp/rp2_desc_e?cjm |
Citation | Canadian Journal Of Microbiology, 2007, v. 53 n. 4, p. 488-495 How to Cite? |
Abstract | Using a combination of high-performance ion chromatography analysis and kinetic studies, the pathway of myo-inositol hexakisphosphate dephosphorylation by the P-propeller phytase of Shewanella oneidensis was established, which was then compared with that of Bacillus subtilis 168, Bacillus amyloliquefaciens ATCC 15841, and B. amyloliquefaciens 45 β-propeller phytases. The data demonstrate that all of these β-propeller phytases dephosphorylate myo-inositol hexakisphosphate in a stereospecific way by sequential removal of phosphate groups via D-Ins(1,2,4,5,6)P 5, Ins(2,4,5,6)P 4 to finally Ins(2,4,6)P 3. Thus, the β-propeller phytases prefer the hydrolysis of every second phosphate over that of adjacent ones. This finding does not support previous phytate degradation models proposed by J. Kerovuo, J. Rouvinen, and F. Hatzack (2000. Biochem. J. 352: 623-628) and R. Greiner, A. Farouk, M. Larsson Alminger, and N.G. Carlsson (2002. Can. J. Microbiol. 48: 986-994), but seems to fit with the structural model given by S. Shin, N.C. Ha, B.C. Oh, T.K. Oh, and B.H. Oh (2001. Structure, 9: 851-858). © 2007 NRC. |
Persistent Identifier | http://hdl.handle.net/10722/57196 |
ISSN | 2023 Impact Factor: 1.8 2023 SCImago Journal Rankings: 0.539 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Greiner, R | en_HK |
dc.contributor.author | Lim, BL | en_HK |
dc.contributor.author | Cheng, C | en_HK |
dc.contributor.author | Carlsson, NG | en_HK |
dc.date.accessioned | 2010-04-12T01:29:13Z | - |
dc.date.available | 2010-04-12T01:29:13Z | - |
dc.date.issued | 2007 | en_HK |
dc.identifier.citation | Canadian Journal Of Microbiology, 2007, v. 53 n. 4, p. 488-495 | en_HK |
dc.identifier.issn | 0008-4166 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/57196 | - |
dc.description.abstract | Using a combination of high-performance ion chromatography analysis and kinetic studies, the pathway of myo-inositol hexakisphosphate dephosphorylation by the P-propeller phytase of Shewanella oneidensis was established, which was then compared with that of Bacillus subtilis 168, Bacillus amyloliquefaciens ATCC 15841, and B. amyloliquefaciens 45 β-propeller phytases. The data demonstrate that all of these β-propeller phytases dephosphorylate myo-inositol hexakisphosphate in a stereospecific way by sequential removal of phosphate groups via D-Ins(1,2,4,5,6)P 5, Ins(2,4,5,6)P 4 to finally Ins(2,4,6)P 3. Thus, the β-propeller phytases prefer the hydrolysis of every second phosphate over that of adjacent ones. This finding does not support previous phytate degradation models proposed by J. Kerovuo, J. Rouvinen, and F. Hatzack (2000. Biochem. J. 352: 623-628) and R. Greiner, A. Farouk, M. Larsson Alminger, and N.G. Carlsson (2002. Can. J. Microbiol. 48: 986-994), but seems to fit with the structural model given by S. Shin, N.C. Ha, B.C. Oh, T.K. Oh, and B.H. Oh (2001. Structure, 9: 851-858). © 2007 NRC. | en_HK |
dc.language | eng | en_HK |
dc.publisher | N R C Research Press. The Journal's web site is located at http://pubs.nrc-cnrc.gc.ca/cgi-bin/rp/rp2_desc_e?cjm | en_HK |
dc.relation.ispartof | Canadian Journal of Microbiology | en_HK |
dc.rights | Canadian Journal of Microbiology. Copyright © N R C Research Press. | en_HK |
dc.rights | Publisher PDF may be archived 6 months after publication | en_HK |
dc.subject | β-propeller phytase | en_HK |
dc.subject | Bacillus spp. | en_HK |
dc.subject | myo-inositol phosphate isomers | en_HK |
dc.subject | Phytate degradation | en_HK |
dc.subject | Shewanella oneidensis | en_HK |
dc.title | Pathway of phytate dephosphorylation by β-propeller phytases of different origins | en_HK |
dc.type | Article | en_HK |
dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0008-4166&volume=53&issue=4&spage=488&epage=495&date=2007&atitle=Pathway+of+phytate+dephosphorylation+by+β-propeller+phytases+of+different+origins | en_HK |
dc.identifier.email | Lim, BL: bllim@hkucc.hku.hk | en_HK |
dc.identifier.authority | Lim, BL=rp00744 | en_HK |
dc.description.nature | published_or_final_version | en_HK |
dc.identifier.doi | 10.1139/W07-015 | en_HK |
dc.identifier.pmid | 17612603 | - |
dc.identifier.scopus | eid_2-s2.0-34347382434 | en_HK |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-34347382434&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 53 | en_HK |
dc.identifier.issue | 4 | en_HK |
dc.identifier.spage | 488 | en_HK |
dc.identifier.epage | 495 | en_HK |
dc.identifier.isi | WOS:000247769300004 | - |
dc.publisher.place | Canada | en_HK |
dc.identifier.scopusauthorid | Greiner, R=7102873664 | en_HK |
dc.identifier.scopusauthorid | Lim, BL=7201983917 | en_HK |
dc.identifier.scopusauthorid | Cheng, C=7404797223 | en_HK |
dc.identifier.scopusauthorid | Carlsson, NG=7005250998 | en_HK |
dc.identifier.issnl | 0008-4166 | - |