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Article: Small noncoding RNA GcvB is a novel regulator of acid resistance in Escherichia coli

TitleSmall noncoding RNA GcvB is a novel regulator of acid resistance in Escherichia coli
Authors
Jin, YWatt, RMDanchin, AHuang, JD
Issue Date2009
PublisherBioMed Central Ltd. The Journal's web site is located at http://www.biomedcentral.com/bmcgenomics/
Citation
Bmc Genomics, 2009, v. 10 How to Cite?
DOI: http://dx.doi.org/10.1186/1471-2164-10-165
AbstractBackground: The low pH environment of the human stomach is lethal for most microorganisms; but not Escherichia coli, which can tolerate extreme acid stress. Acid resistance in E. coli is hierarchically controlled by numerous regulators among which are small noncoding RNAs (sncRNA). Results: In this study, we individually deleted seventy-nine sncRNA genes from the E. coli K12-MG1655 chromosome, and established a single-sncRNA gene knockout library. By systematically screening the sncRNA mutant library, we show that the sncRNA GcvB is a novel regulator of acid resistance in E. coli. We demonstrate that GcvB enhances the ability of E. coli to survive low pH by upregulating the levels of the alternate sigma factor RpoS. Conclusion: GcvB positively regulates acid resistance by affecting RpoS expression. These data advance our understanding of the sncRNA regulatory network involved in modulating acid resistance in E. coli. © 2009 Jin et al; licensee BioMed Central Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/58132
ISSN
1471-2164
2021 Impact Factor: 4.547
2020 SCImago Journal Rankings: 1.547
ISI Accession Number ID
WOS:000265794300001
Funding AgencyGrant Number
HKU CRCG Seed Funding Programme for Basic Research
Council of Hong KongHKU 7485/06M
PROBACTYSCT-2006-029104
Funding Information:

We thank D. L. Court for providing plasmid pSim6 for the recombineering experiments. This work was supported by HKU CRCG Seed Funding Programme for Basic Research and by a grant (HKU 7485/06M) from the Research Grant Council of Hong Kong to JDH. AD acknowledges support from the PROBACTYS programme, grant CT-2006-029104 in an effort to define genes essential for the construction of a synthetic cell.

References
References in Scopus
Grants
Towards a visualized proteome of <I>Escherichia coli</I>

 

DC FieldValueLanguage
dc.contributor.authorJin, Yen_HK
dc.contributor.authorWatt, RMen_HK
dc.contributor.authorDanchin, Aen_HK
dc.contributor.authorHuang, JDen_HK
dc.date.accessioned2010-05-31T03:24:20Z-
dc.date.available2010-05-31T03:24:20Z-
dc.date.issued2009en_HK
dc.identifier.citationBmc Genomics, 2009, v. 10en_HK
dc.identifier.issn1471-2164en_HK
dc.identifier.urihttp://hdl.handle.net/10722/58132-
dc.description.abstractBackground: The low pH environment of the human stomach is lethal for most microorganisms; but not Escherichia coli, which can tolerate extreme acid stress. Acid resistance in E. coli is hierarchically controlled by numerous regulators among which are small noncoding RNAs (sncRNA). Results: In this study, we individually deleted seventy-nine sncRNA genes from the E. coli K12-MG1655 chromosome, and established a single-sncRNA gene knockout library. By systematically screening the sncRNA mutant library, we show that the sncRNA GcvB is a novel regulator of acid resistance in E. coli. We demonstrate that GcvB enhances the ability of E. coli to survive low pH by upregulating the levels of the alternate sigma factor RpoS. Conclusion: GcvB positively regulates acid resistance by affecting RpoS expression. These data advance our understanding of the sncRNA regulatory network involved in modulating acid resistance in E. coli. © 2009 Jin et al; licensee BioMed Central Ltd.en_HK
dc.languageengen_HK
dc.publisherBioMed Central Ltd. The Journal's web site is located at http://www.biomedcentral.com/bmcgenomics/en_HK
dc.relation.ispartofBMC Genomicsen_HK
dc.rightsB M C Genomics. Copyright © BioMed Central Ltd.en_HK
dc.subject.meshAdaptation, Physiologicalen_HK
dc.subject.meshBacterial Proteins - genetics - metabolismen_HK
dc.subject.meshCarrier Proteins - genetics - metabolismen_HK
dc.subject.meshEscherichia coli - genetics - metabolismen_HK
dc.subject.meshEscherichia coli Proteins - genetics - metabolismen_HK
dc.subject.meshGene Expression Regulation, Bacterialen_HK
dc.subject.meshGene Knockout Techniquesen_HK
dc.subject.meshHydrogen-Ion Concentrationen_HK
dc.subject.meshLipoproteins - genetics - metabolismen_HK
dc.subject.meshMutationen_HK
dc.subject.meshRNA, Untranslated - genetics - metabolismen_HK
dc.subject.meshSigma Factor - genetics - metabolismen_HK
dc.titleSmall noncoding RNA GcvB is a novel regulator of acid resistance in Escherichia colien_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1471-2164&volume=10&spage=165&epage=171&date=2009&atitle=Small+noncoding+RNA+GcvB+is+a+novel+regulator+of+acid+resistance+in+Escherichia+colien_HK
dc.identifier.emailWatt, RM:rmwatt@hku.hken_HK
dc.identifier.emailHuang, JD:jdhuang@hkucc.hku.hken_HK
dc.identifier.authorityWatt, RM=rp00043en_HK
dc.identifier.authorityHuang, JD=rp00451en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1186/1471-2164-10-165en_HK
dc.identifier.pmid19379489-
dc.identifier.scopuseid_2-s2.0-65649087919en_HK
dc.identifier.hkuros155302en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-65649087919&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume10en_HK
dc.identifier.isiWOS:000265794300001-
dc.publisher.placeUnited Kingdomen_HK
dc.relation.projectTowards a visualized proteome of <I>Escherichia coli</I>-
dc.identifier.scopusauthoridJin, Y=29467538100en_HK
dc.identifier.scopusauthoridWatt, RM=7102907536en_HK
dc.identifier.scopusauthoridDanchin, A=7103235597en_HK
dc.identifier.scopusauthoridHuang, JD=8108660600en_HK
dc.identifier.citeulike4373078-
dc.identifier.issnl1471-2164-

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