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Article: The autocrine human secreted PDZ domain-containing protein 2 (sPDZD2) induces senescence or quiescence of prostate, breast and liver cancer cells via transcriptional activation of p53

TitleThe autocrine human secreted PDZ domain-containing protein 2 (sPDZD2) induces senescence or quiescence of prostate, breast and liver cancer cells via transcriptional activation of p53
Authors
Tam, CWLiu, VWSLeung, WYYao, KMShiu, SYW
KeywordsBreast
Liver
p53
Prostate
sPDZD2
Issue Date2008
PublisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/canlet
Citation
Cancer Letters, 2008, v. 271 n. 1, p. 64-80 How to Cite?
DOI: http://dx.doi.org/10.1016/j.canlet.2008.05.047
AbstractTumor suppressive actions of the autocrine human secreted PDZ domain-containing protein 2 (sPDZD2) have been reported, but the mechanisms remain enigmatic. Here, we showed that sPDZD2 induced senescence of prostate cancer DU145 cells, quiescence of breast cancer MCF-7 and liver cancer Hep-G2 cells, via transcriptional activation of mutant or wild-type p53. Furthermore, sPDZD2 sensitized mutant p53-positive DU145 cells and wild-type p53-positive MCF-7 cells to apoptosis induction through genotoxic stress imposed by sub-lethal concentration of hydrogen peroxide. Together, our findings suggest a potential autocrine pathway of p53 activation by transcriptional regulation, and a new approach to reactivate p53 for cancer therapy. © 2008 Elsevier Ireland Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/58275
ISSN
0304-3835
2023 Impact Factor: 9.1
2023 SCImago Journal Rankings: 2.595
ISI Accession Number ID
WOS:000260987900007
Funding AgencyGrant Number
University of Hong Kong
HKU7474/04M
200507176053
200611159071
HKU7580/05M
Funding Information:

We thank Prof. S. Sukumar (Johns Hopkins Oncology Centre, Baltimore, Maryland) for the gift of p53 reporter construct -2400-p53-Luc, Dr. E. Fearon (University of Michigan School of Medicine, Ann Arbor, Michigan) for providing the sequence information of the antisense locked nucleic acid (LNA) oligonucleotides and Dr. N. S. Wong (Department of Biochemistry, The University of Hong Kong) for providing the human caspase-3 cDNA. This work was supported by research grants HKU7474/04M (to K.-M.Y.), #200507176053, #200611159071 and HKU7580/05M (to S.Y.W.S.). C.W.T. is supported by a postgraduate studentship of The University of Hong Kong and the data presented were derived from part of his Ph.D. thesis work.

References
References in Scopus
Grants
Overexpression of sPDZD2 in prostate cancer cells
Structure-function studies of sPDZD2 protein and identification of its cellular targets

 

DC FieldValueLanguage
dc.contributor.authorTam, CWen_HK
dc.contributor.authorLiu, VWSen_HK
dc.contributor.authorLeung, WYen_HK
dc.contributor.authorYao, KMen_HK
dc.contributor.authorShiu, SYWen_HK
dc.date.accessioned2010-05-31T03:27:15Z-
dc.date.available2010-05-31T03:27:15Z-
dc.date.issued2008en_HK
dc.identifier.citationCancer Letters, 2008, v. 271 n. 1, p. 64-80en_HK
dc.identifier.issn0304-3835en_HK
dc.identifier.urihttp://hdl.handle.net/10722/58275-
dc.description.abstractTumor suppressive actions of the autocrine human secreted PDZ domain-containing protein 2 (sPDZD2) have been reported, but the mechanisms remain enigmatic. Here, we showed that sPDZD2 induced senescence of prostate cancer DU145 cells, quiescence of breast cancer MCF-7 and liver cancer Hep-G2 cells, via transcriptional activation of mutant or wild-type p53. Furthermore, sPDZD2 sensitized mutant p53-positive DU145 cells and wild-type p53-positive MCF-7 cells to apoptosis induction through genotoxic stress imposed by sub-lethal concentration of hydrogen peroxide. Together, our findings suggest a potential autocrine pathway of p53 activation by transcriptional regulation, and a new approach to reactivate p53 for cancer therapy. © 2008 Elsevier Ireland Ltd. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/canleten_HK
dc.relation.ispartofCancer Lettersen_HK
dc.rightsCancer Letters. Copyright © Elsevier Ireland Ltd.en_HK
dc.subjectBreasten_HK
dc.subjectLiveren_HK
dc.subjectp53en_HK
dc.subjectProstateen_HK
dc.subjectsPDZD2en_HK
dc.subject.meshAdaptor Proteins, Signal Transducing - physiologyen_HK
dc.subject.meshApoptosisen_HK
dc.subject.meshBase Sequenceen_HK
dc.subject.meshBreast Neoplasms - metabolism - pathologyen_HK
dc.subject.meshCell Aging - physiologyen_HK
dc.subject.meshCell Line, Tumoren_HK
dc.subject.meshCell Proliferationen_HK
dc.subject.meshDNA Primersen_HK
dc.subject.meshHumansen_HK
dc.subject.meshLiver Neoplasms - metabolism - pathologyen_HK
dc.subject.meshMaleen_HK
dc.subject.meshNeoplasm Proteins - physiologyen_HK
dc.subject.meshProstatic Neoplasms - metabolism - pathologyen_HK
dc.subject.meshRNA Interferenceen_HK
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_HK
dc.subject.meshTranscriptional Activation - physiologyen_HK
dc.subject.meshTumor Suppressor Protein p53 - physiologyen_HK
dc.titleThe autocrine human secreted PDZ domain-containing protein 2 (sPDZD2) induces senescence or quiescence of prostate, breast and liver cancer cells via transcriptional activation of p53en_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0304-3835&volume=271&spage=64&epage=80&date=2008&atitle=The+autocrine+human+secreted+PDZ+domain-containing+protein+2+(sPDZD2)+induces+senescence+or+quiescence+of+prostate,+breast+and+liver+cancer+cells+via+transcriptional+activation+of+p53en_HK
dc.identifier.emailLiu, VWS: vwsliu@hkusua.hku.hken_HK
dc.identifier.emailYao, KM: kmyao@hku.hken_HK
dc.identifier.emailShiu, SYW: sywshiu@hkucc.hku.hken_HK
dc.identifier.authorityLiu, VWS=rp00341en_HK
dc.identifier.authorityYao, KM=rp00344en_HK
dc.identifier.authorityShiu, SYW=rp00384en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.canlet.2008.05.047en_HK
dc.identifier.pmid18639375-
dc.identifier.scopuseid_2-s2.0-53049106876en_HK
dc.identifier.hkuros148671en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-53049106876&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume271en_HK
dc.identifier.issue1en_HK
dc.identifier.spage64en_HK
dc.identifier.epage80en_HK
dc.identifier.isiWOS:000260987900007-
dc.publisher.placeIrelanden_HK
dc.relation.projectOverexpression of sPDZD2 in prostate cancer cells-
dc.relation.projectStructure-function studies of sPDZD2 protein and identification of its cellular targets-
dc.identifier.scopusauthoridTam, CW=7201442977en_HK
dc.identifier.scopusauthoridLiu, VWS=7006405113en_HK
dc.identifier.scopusauthoridLeung, WY=7201504543en_HK
dc.identifier.scopusauthoridYao, KM=7403234578en_HK
dc.identifier.scopusauthoridShiu, SYW=7005550655en_HK
dc.identifier.issnl0304-3835-

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