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Article: Role of monoamine oxidases in the exaggerated 5-hydroxytryptamine-induced tension development of human isolated preeclamptic umbilical artery

TitleRole of monoamine oxidases in the exaggerated 5-hydroxytryptamine-induced tension development of human isolated preeclamptic umbilical artery
Authors
Seto, SWLam, HYLau, WSAu, ALSLam, TYChim, SSCNgai, SMChan, SWLeung, TYYeung, JHKKong, SKLeung, GPHLee, SMYKwan, YW
Keywords5-hydroxytryptamine
Monoamine oxidase
Preeclampsia
Umbilical artery
Issue Date2009
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/ejphar
Citation
European Journal Of Pharmacology, 2009, v. 605 n. 1-3, p. 129-137 How to Cite?
DOI: http://dx.doi.org/10.1016/j.ejphar.2008.12.054
AbstractWe investigated the role(s) of monoamine oxidases (MAOs) on the altered 5-hydroxytryptamine (5-HT, serotonin)-induced tension development of the isolated umbilical artery of preeclamptic pregnancy of Chinese women. An enhanced 5-HT-induced tension development of the umbilical artery of preeclamptic pregnancy was observed when compared with that of normal pregnancy. The enhanced component of 5-HT-induced tension development was eradicated by clorgyline (a MAO-A inhibitor). Blockade of eNOS (endothelial isoform nitric oxide synthase) (Nω-nitro-l-arginine methyl ester), 5-HT transporter (citalopram), 5-HT receptor subtypes (5HT2B, SB 204741; 5-HT2C, RS 102221; 5-HT7, SB 269970), and endothelium denudation of the umbilical artery of normal pregnancy mimicked the enhanced 5-HT-induced tension development as observed in the preeclamptic tissues. In contrast, no apparent changes in 5-HT-induced tension development of the umbilical artery of preeclamptic pregnancy were observed with the same pharmacological manipulations. A decreased protein expression levels of MAO-A and eNOS (no iNOS and MAO-B expression was detected) and no change in caveolin-1 and 5-HT transporter expression were demonstrated in the umbilical artery (endothelium intact) lysate of preeclamptic pregnancy, compared to that of the umbilical artery of normal pregnancy. Thus, in the umbilical artery of preeclamptic pregnancy, a decrease of MAO-A and eNOS protein expression levels are probably associated with, or responsible for, the exaggerated 5-HT-induced tension development. © 2009 Elsevier B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/59539
ISSN
0014-2999
2023 Impact Factor: 4.2
2023 SCImago Journal Rankings: 1.055
ISI Accession Number ID
WOS:000264407300021
Funding AgencyGrant Number
Li Ka Shing Institute of Health Sciences and Institute of Vascular Medicine
Faculty of Medicine
Chinese University of Hong Kong2401149
2041231
2401296
2007.1.079
RGC4107/01M
4166/02M
2140565
Funding Information:

We thank the excellent assistance provided by nurses of the operating theatres of The Prince of Wales Hospital (Hong Kong), and women who agreed to donate the umbilical cords for this study. We are grateful to Li Ka Shing Institute of Health Sciences and Institute of Vascular Medicine (Faculty of Medicine, The Chinese University of Hong Kong) for financial supports (to YW Kwan). This project is financially supported by the RGC Earmarked Grants of Hong Kong (Ref. #: 4107/01M; 4166/02M, project code: 2140565), and Direct Grants for Research (The Chinese University of Hong Kong) (Reference no.: 2401149; Project code/ID: 2041231; 2401296; Reference no.: 2007.1.079). Mr. SW Seto, Ms. Alice LS Au and Ms TY Lam are recipients of post-graduate studentships of the Department of Pharmacology (The Chinese University of Hong Kong). Provision of the Student Campus Work Scheme by the Chou's Foundation Fund, and the Student Training & Campus Service Scheme (Shaw College, The Chinese University of Hong Kong) is also appreciated. Proofreading of the manuscript by Mr. Alan HY Lam is also acknowledged.

References
References in Scopus

 

DC FieldValueLanguage
dc.contributor.authorSeto, SWen_HK
dc.contributor.authorLam, HYen_HK
dc.contributor.authorLau, WSen_HK
dc.contributor.authorAu, ALSen_HK
dc.contributor.authorLam, TYen_HK
dc.contributor.authorChim, SSCen_HK
dc.contributor.authorNgai, SMen_HK
dc.contributor.authorChan, SWen_HK
dc.contributor.authorLeung, TYen_HK
dc.contributor.authorYeung, JHKen_HK
dc.contributor.authorKong, SKen_HK
dc.contributor.authorLeung, GPHen_HK
dc.contributor.authorLee, SMYen_HK
dc.contributor.authorKwan, YWen_HK
dc.date.accessioned2010-05-31T03:52:19Z-
dc.date.available2010-05-31T03:52:19Z-
dc.date.issued2009en_HK
dc.identifier.citationEuropean Journal Of Pharmacology, 2009, v. 605 n. 1-3, p. 129-137en_HK
dc.identifier.issn0014-2999en_HK
dc.identifier.urihttp://hdl.handle.net/10722/59539-
dc.description.abstractWe investigated the role(s) of monoamine oxidases (MAOs) on the altered 5-hydroxytryptamine (5-HT, serotonin)-induced tension development of the isolated umbilical artery of preeclamptic pregnancy of Chinese women. An enhanced 5-HT-induced tension development of the umbilical artery of preeclamptic pregnancy was observed when compared with that of normal pregnancy. The enhanced component of 5-HT-induced tension development was eradicated by clorgyline (a MAO-A inhibitor). Blockade of eNOS (endothelial isoform nitric oxide synthase) (Nω-nitro-l-arginine methyl ester), 5-HT transporter (citalopram), 5-HT receptor subtypes (5HT2B, SB 204741; 5-HT2C, RS 102221; 5-HT7, SB 269970), and endothelium denudation of the umbilical artery of normal pregnancy mimicked the enhanced 5-HT-induced tension development as observed in the preeclamptic tissues. In contrast, no apparent changes in 5-HT-induced tension development of the umbilical artery of preeclamptic pregnancy were observed with the same pharmacological manipulations. A decreased protein expression levels of MAO-A and eNOS (no iNOS and MAO-B expression was detected) and no change in caveolin-1 and 5-HT transporter expression were demonstrated in the umbilical artery (endothelium intact) lysate of preeclamptic pregnancy, compared to that of the umbilical artery of normal pregnancy. Thus, in the umbilical artery of preeclamptic pregnancy, a decrease of MAO-A and eNOS protein expression levels are probably associated with, or responsible for, the exaggerated 5-HT-induced tension development. © 2009 Elsevier B.V. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/ejpharen_HK
dc.relation.ispartofEuropean Journal of Pharmacologyen_HK
dc.rightsEuropean Journal of Pharmacology . Copyright © Elsevier BV.en_HK
dc.subject5-hydroxytryptamineen_HK
dc.subjectMonoamine oxidaseen_HK
dc.subjectPreeclampsiaen_HK
dc.subjectUmbilical arteryen_HK
dc.titleRole of monoamine oxidases in the exaggerated 5-hydroxytryptamine-induced tension development of human isolated preeclamptic umbilical arteryen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0014-2999&volume=605&spage=129&epage=137&date=2009&atitle=Role+of+monoamine+oxidases+in+the+exaggerated+5-hydroxytryptamine-induced+tension+development+of+human+isolated+preeclamptic+umbilical+arteryen_HK
dc.identifier.emailLeung, GPH: gphleung@hkucc.hku.hken_HK
dc.identifier.authorityLeung, GPH=rp00234en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.ejphar.2008.12.054en_HK
dc.identifier.pmid19248248-
dc.identifier.scopuseid_2-s2.0-60349130008en_HK
dc.identifier.hkuros157518en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-60349130008&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume605en_HK
dc.identifier.issue1-3en_HK
dc.identifier.spage129en_HK
dc.identifier.epage137en_HK
dc.identifier.isiWOS:000264407300021-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridSeto, SW=9941482400en_HK
dc.identifier.scopusauthoridLam, HY=36986585100en_HK
dc.identifier.scopusauthoridLau, WS=26024807300en_HK
dc.identifier.scopusauthoridAu, ALS=7005391144en_HK
dc.identifier.scopusauthoridLam, TY=18134321000en_HK
dc.identifier.scopusauthoridChim, SSC=6701728226en_HK
dc.identifier.scopusauthoridNgai, SM=7006074219en_HK
dc.identifier.scopusauthoridChan, SW=7404255670en_HK
dc.identifier.scopusauthoridLeung, TY=7202110959en_HK
dc.identifier.scopusauthoridYeung, JHK=7006803824en_HK
dc.identifier.scopusauthoridKong, SK=7203043875en_HK
dc.identifier.scopusauthoridLeung, GPH=35963668200en_HK
dc.identifier.scopusauthoridLee, SMY=35233892600en_HK
dc.identifier.scopusauthoridKwan, YW=7005662153en_HK
dc.identifier.issnl0014-2999-

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