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- Publisher Website: 10.1002/gene.20062
- Scopus: eid_2-s2.0-10644262893
- PMID: 15354293
- WOS: WOS:000224257500007
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Article: Highly restricted expression of Cre recombinase in cerebellar Purkinje cells
Title | Highly restricted expression of Cre recombinase in cerebellar Purkinje cells |
---|---|
Authors | |
Keywords | BAC Cerebellum Cre recombinase L7 Pcp2 Purkinje cells Transgenic mouse |
Issue Date | 2004 |
Publisher | John Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/68503812 |
Citation | Genesis, 2004, v. 40 n. 1, p. 45-51 How to Cite? |
Abstract | The Purkinje neuron, one of the most fascinating components of the cerebellar cortex, is involved in motor learning, motor coordination, and cognitive function. Purkinje cell protein 2 (Pcp2/L7) expression is highly restricted to Purkinje and retinal bipolar cells, where it has been exploited to enable highly specific, Cre recombinase-mediated, site-specific recombination. Previous studies showed that mice carrying a Cre transgene produced by insertion of Cre cDNA into a small 2.88-kb Pcp2 DNA fragment expressed Cre in Purkinje cells; however, some Cre activity was also observed outside the target tissues. Here, we used Red-mediated recombineering to insert Cre cDNA into a 173-kb BAC carrying the entire intact Pcp2 gene, and characterize the resultant BAC/Cre transgenic mice for Cre expression. We show that BAC/Cre transgenic mice have exclusive Cre expression in Purkinje and bipolar cells and nowhere else. These mice will facilitate Purkinje cell and retinal bipolar cell-specific genetic manipulation. © 2004 Wiley-Liss, Inc. |
Persistent Identifier | http://hdl.handle.net/10722/67378 |
ISSN | 2023 Impact Factor: 2.4 2023 SCImago Journal Rankings: 0.757 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Zhang, XM | en_HK |
dc.contributor.author | Ng, AHL | en_HK |
dc.contributor.author | Tanner, JA | en_HK |
dc.contributor.author | Wu, WT | en_HK |
dc.contributor.author | Copeland, NG | en_HK |
dc.contributor.author | Jenkins, NA | en_HK |
dc.contributor.author | Huang, JD | en_HK |
dc.date.accessioned | 2010-09-06T05:54:36Z | - |
dc.date.available | 2010-09-06T05:54:36Z | - |
dc.date.issued | 2004 | en_HK |
dc.identifier.citation | Genesis, 2004, v. 40 n. 1, p. 45-51 | en_HK |
dc.identifier.issn | 1526-954X | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/67378 | - |
dc.description.abstract | The Purkinje neuron, one of the most fascinating components of the cerebellar cortex, is involved in motor learning, motor coordination, and cognitive function. Purkinje cell protein 2 (Pcp2/L7) expression is highly restricted to Purkinje and retinal bipolar cells, where it has been exploited to enable highly specific, Cre recombinase-mediated, site-specific recombination. Previous studies showed that mice carrying a Cre transgene produced by insertion of Cre cDNA into a small 2.88-kb Pcp2 DNA fragment expressed Cre in Purkinje cells; however, some Cre activity was also observed outside the target tissues. Here, we used Red-mediated recombineering to insert Cre cDNA into a 173-kb BAC carrying the entire intact Pcp2 gene, and characterize the resultant BAC/Cre transgenic mice for Cre expression. We show that BAC/Cre transgenic mice have exclusive Cre expression in Purkinje and bipolar cells and nowhere else. These mice will facilitate Purkinje cell and retinal bipolar cell-specific genetic manipulation. © 2004 Wiley-Liss, Inc. | en_HK |
dc.language | eng | en_HK |
dc.publisher | John Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/68503812 | en_HK |
dc.relation.ispartof | Genesis | en_HK |
dc.subject | BAC | en_HK |
dc.subject | Cerebellum | en_HK |
dc.subject | Cre recombinase | en_HK |
dc.subject | L7 | en_HK |
dc.subject | Pcp2 | en_HK |
dc.subject | Purkinje cells | en_HK |
dc.subject | Transgenic mouse | en_HK |
dc.subject.mesh | Animals | en_HK |
dc.subject.mesh | Base Sequence | en_HK |
dc.subject.mesh | Cerebellum - enzymology | en_HK |
dc.subject.mesh | Chromosomes, Artificial, Bacterial | en_HK |
dc.subject.mesh | DNA Primers | en_HK |
dc.subject.mesh | DNA, Complementary - genetics | en_HK |
dc.subject.mesh | Gene Expression Regulation, Enzymologic | en_HK |
dc.subject.mesh | Integrases - genetics | en_HK |
dc.subject.mesh | Male | en_HK |
dc.subject.mesh | Mice | en_HK |
dc.subject.mesh | Mice, Transgenic | en_HK |
dc.subject.mesh | Motor Activity | en_HK |
dc.subject.mesh | Purkinje Cells - enzymology | en_HK |
dc.title | Highly restricted expression of Cre recombinase in cerebellar Purkinje cells | en_HK |
dc.type | Article | en_HK |
dc.identifier.email | Tanner, JA:jatanner@hku.hk | en_HK |
dc.identifier.email | Wu, WT:wtwu@hkucc.hku.hk | en_HK |
dc.identifier.email | Huang, JD:jdhuang@hkucc.hku.hk | en_HK |
dc.identifier.authority | Tanner, JA=rp00495 | en_HK |
dc.identifier.authority | Wu, WT=rp00419 | en_HK |
dc.identifier.authority | Huang, JD=rp00451 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1002/gene.20062 | en_HK |
dc.identifier.pmid | 15354293 | en_HK |
dc.identifier.scopus | eid_2-s2.0-10644262893 | en_HK |
dc.identifier.hkuros | 94998 | en_HK |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-10644262893&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 40 | en_HK |
dc.identifier.issue | 1 | en_HK |
dc.identifier.spage | 45 | en_HK |
dc.identifier.epage | 51 | en_HK |
dc.identifier.isi | WOS:000224257500007 | - |
dc.publisher.place | United States | en_HK |
dc.identifier.scopusauthorid | Zhang, XM=37092286900 | en_HK |
dc.identifier.scopusauthorid | Ng, AHL=12445077000 | en_HK |
dc.identifier.scopusauthorid | Tanner, JA=35513993000 | en_HK |
dc.identifier.scopusauthorid | Wu, WT=7407081122 | en_HK |
dc.identifier.scopusauthorid | Copeland, NG=35374759300 | en_HK |
dc.identifier.scopusauthorid | Jenkins, NA=35379887700 | en_HK |
dc.identifier.scopusauthorid | Huang, JD=8108660600 | en_HK |
dc.identifier.issnl | 1526-954X | - |