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- Publisher Website: 10.1007/s00441-002-0689-6
- Scopus: eid_2-s2.0-0037365289
- PMID: 12658449
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Article: Inhibitor of apoptosis proteins and ovarian dysfunction in galactosemic rats
Title | Inhibitor of apoptosis proteins and ovarian dysfunction in galactosemic rats |
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Authors | |
Keywords | Apoptosis Galactosemia Granulosa cells Inhibitor of apoptosis proteins Ovulation Rat (Sprague Dawley) |
Issue Date | 2003 |
Publisher | Springer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00441/index.htm |
Citation | Cell And Tissue Research, 2003, v. 311 n. 3, p. 417-425 How to Cite? |
Abstract | Galactosemia is a genetic disease with deficiency of galactose-1-uridyltransferase, resulting in the accumulation of galactose or galactose-1-phosphate in the blood and tissues. Rats were fed with normal rat chow and with a high-galactose diet for 4 weeks to give control and galactosemic groups, and their ovarian function was studied. The two groups of rats were injected with pregnant mare's serum gonadotrophin (PMSG) and were killed at different time points after human chorionic gonadotrophin (hCG) injection. The number of oocytes ovulated in the controls was significantly higher than in the galactosemic group. Morphometric studies of the ovaries also showed a higher number of corpora lutea in the controls. Western blot analysis of granulosa cells showed that the overall expressions of Fas and FasL were lower in the control group and their expressions of inhibitor of apoptosis proteins (IAPs) were higher than in the galactosemic group, especially at 8 h post hCG injection. TDT-mediated dUTP-biotin nick end-labeling (TUNEL) and immunohistochemical staining of ovarian sections with Ki-67 and IAPs showed more apoptotic granulosa cells in the galactosemic group and the expressions of IAPs in granulosa cells also confirmed the result of the Western blot. These findings support our hypothesis that ovarian dysfunction in galactosemic rats is due to increased apoptosis in granulosa cells of maturing follicles. |
Persistent Identifier | http://hdl.handle.net/10722/67411 |
ISSN | 2023 Impact Factor: 3.2 2023 SCImago Journal Rankings: 0.965 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lai, KW | en_HK |
dc.contributor.author | Cheng, LYL | en_HK |
dc.contributor.author | Cheung, ALM | en_HK |
dc.contributor.author | O, WS | en_HK |
dc.date.accessioned | 2010-09-06T05:54:54Z | - |
dc.date.available | 2010-09-06T05:54:54Z | - |
dc.date.issued | 2003 | en_HK |
dc.identifier.citation | Cell And Tissue Research, 2003, v. 311 n. 3, p. 417-425 | en_HK |
dc.identifier.issn | 0302-766X | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/67411 | - |
dc.description.abstract | Galactosemia is a genetic disease with deficiency of galactose-1-uridyltransferase, resulting in the accumulation of galactose or galactose-1-phosphate in the blood and tissues. Rats were fed with normal rat chow and with a high-galactose diet for 4 weeks to give control and galactosemic groups, and their ovarian function was studied. The two groups of rats were injected with pregnant mare's serum gonadotrophin (PMSG) and were killed at different time points after human chorionic gonadotrophin (hCG) injection. The number of oocytes ovulated in the controls was significantly higher than in the galactosemic group. Morphometric studies of the ovaries also showed a higher number of corpora lutea in the controls. Western blot analysis of granulosa cells showed that the overall expressions of Fas and FasL were lower in the control group and their expressions of inhibitor of apoptosis proteins (IAPs) were higher than in the galactosemic group, especially at 8 h post hCG injection. TDT-mediated dUTP-biotin nick end-labeling (TUNEL) and immunohistochemical staining of ovarian sections with Ki-67 and IAPs showed more apoptotic granulosa cells in the galactosemic group and the expressions of IAPs in granulosa cells also confirmed the result of the Western blot. These findings support our hypothesis that ovarian dysfunction in galactosemic rats is due to increased apoptosis in granulosa cells of maturing follicles. | en_HK |
dc.language | eng | en_HK |
dc.publisher | Springer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00441/index.htm | en_HK |
dc.relation.ispartof | Cell and Tissue Research | en_HK |
dc.subject | Apoptosis | en_HK |
dc.subject | Galactosemia | en_HK |
dc.subject | Granulosa cells | en_HK |
dc.subject | Inhibitor of apoptosis proteins | en_HK |
dc.subject | Ovulation | en_HK |
dc.subject | Rat (Sprague Dawley) | en_HK |
dc.subject.mesh | Animals | en_HK |
dc.subject.mesh | Antigens, CD95 - metabolism | en_HK |
dc.subject.mesh | Apoptosis - physiology | en_HK |
dc.subject.mesh | Cell Survival - physiology | en_HK |
dc.subject.mesh | Chorionic Gonadotropin - pharmacology | en_HK |
dc.subject.mesh | Disease Models, Animal | en_HK |
dc.subject.mesh | Fas Ligand Protein | en_HK |
dc.subject.mesh | Female | en_HK |
dc.subject.mesh | Food, Formulated - adverse effects | en_HK |
dc.subject.mesh | Galactose - adverse effects - metabolism | en_HK |
dc.subject.mesh | Galactosemias - complications - metabolism - pathology | en_HK |
dc.subject.mesh | Gonadotropins, Equine - pharmacology | en_HK |
dc.subject.mesh | Granulosa Cells - metabolism - pathology | en_HK |
dc.subject.mesh | Inhibitor of Apoptosis Proteins | en_HK |
dc.subject.mesh | Membrane Glycoproteins - metabolism | en_HK |
dc.subject.mesh | Ovarian Diseases - etiology - metabolism - pathology | en_HK |
dc.subject.mesh | Proteins - metabolism | en_HK |
dc.subject.mesh | Rats | en_HK |
dc.subject.mesh | Rats, Sprague-Dawley | en_HK |
dc.title | Inhibitor of apoptosis proteins and ovarian dysfunction in galactosemic rats | en_HK |
dc.type | Article | en_HK |
dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0302-766X&volume=311&spage=417&epage=425&date=2003&atitle=Inhibitor+of+apoptosis+proteins+and+ovarian+dysfunction+in+galactosemic+rats | en_HK |
dc.identifier.email | Cheng, LYL:lcheng@hkucc.hku.hk | en_HK |
dc.identifier.email | Cheung, ALM:lmcheung@hkucc.hku.hk | en_HK |
dc.identifier.email | O, WS:owaisum@hkucc.hku.hk | en_HK |
dc.identifier.authority | Cheng, LYL=rp00317 | en_HK |
dc.identifier.authority | Cheung, ALM=rp00332 | en_HK |
dc.identifier.authority | O, WS=rp00315 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1007/s00441-002-0689-6 | - |
dc.identifier.pmid | 12658449 | - |
dc.identifier.scopus | eid_2-s2.0-0037365289 | en_HK |
dc.identifier.hkuros | 76297 | en_HK |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0037365289&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 311 | en_HK |
dc.identifier.issue | 3 | en_HK |
dc.identifier.spage | 417 | en_HK |
dc.identifier.epage | 425 | en_HK |
dc.identifier.isi | WOS:000182409600015 | - |
dc.publisher.place | Germany | en_HK |
dc.identifier.scopusauthorid | Lai, KW=7402135541 | en_HK |
dc.identifier.scopusauthorid | Cheng, LYL=7403337122 | en_HK |
dc.identifier.scopusauthorid | Cheung, ALM=7401806497 | en_HK |
dc.identifier.scopusauthorid | O, WS=6701729369 | en_HK |
dc.identifier.issnl | 0302-766X | - |