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Article: Identification of genes differentially expressed in nasopharyngeal carcinoma by messenger RNA differential display

TitleIdentification of genes differentially expressed in nasopharyngeal carcinoma by messenger RNA differential display
Authors
KeywordsDifferential display
Nasopharyngeal carcinoma
Issue Date1998
PublisherSpandidos Publications. The Journal's web site is located at http://www.spandidos-publications.com/ijo/
Citation
International Journal Of Oncology, 1998, v. 13 n. 1, p. 85-89 How to Cite?
AbstractWe have applied the mRNA differential display method to compare and analyze mRNAs prepared from five normal nasopharyngeal epithelial cell cultures and five nasopharyngeal carcinoma cell lines. A total of 24 differential display experiments was performed using different combinations of PCR primers. Sixty-nine cDNA fragments differentially expressed in either normal or malignant nasopharyngeal epithelial cells were identified. Subsequent cloning and sequencing of these differentially expressed cDNA fragments resulted in the identification of seventeen distinct sequences. Seven of these sequences were shown to be novel cDNA sequences not previously reported. Ten of the remaining cDNA fragments showed sequence homology to previously reported genes. Differential expression of four of these seventeen cDNA fragments in normal nasopharyngeal epithelial cells was confirmed by reverse Northern hybridization. One of these cloned cDNA fragments is a novel cDNA sequence while the other three matched to previously reported cDNA sequences involved in cell growth and migration. Homologous sequences identified to be differentially expressed in normal nasopharyngeal epithelial cells in this study are: human 26 kDa cell surface protein (TAPA-1) mRNA, NF- E2 like basic leucine zipper transcriptional activator and the human bullous pemphigoid antigen. The mRNA differential display is a useful tool to identify candidate genes involved in the pathogenesis of nasopharyngeal carcinoma.
Persistent Identifierhttp://hdl.handle.net/10722/67459
ISSN
2023 Impact Factor: 4.5
2023 SCImago Journal Rankings: 1.099
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorFung, LFen_HK
dc.contributor.authorYuen, PWen_HK
dc.contributor.authorWei, Wen_HK
dc.contributor.authorKwan, HSen_HK
dc.contributor.authorTsao, SWen_HK
dc.date.accessioned2010-09-06T05:55:19Z-
dc.date.available2010-09-06T05:55:19Z-
dc.date.issued1998en_HK
dc.identifier.citationInternational Journal Of Oncology, 1998, v. 13 n. 1, p. 85-89en_HK
dc.identifier.issn1019-6439en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67459-
dc.description.abstractWe have applied the mRNA differential display method to compare and analyze mRNAs prepared from five normal nasopharyngeal epithelial cell cultures and five nasopharyngeal carcinoma cell lines. A total of 24 differential display experiments was performed using different combinations of PCR primers. Sixty-nine cDNA fragments differentially expressed in either normal or malignant nasopharyngeal epithelial cells were identified. Subsequent cloning and sequencing of these differentially expressed cDNA fragments resulted in the identification of seventeen distinct sequences. Seven of these sequences were shown to be novel cDNA sequences not previously reported. Ten of the remaining cDNA fragments showed sequence homology to previously reported genes. Differential expression of four of these seventeen cDNA fragments in normal nasopharyngeal epithelial cells was confirmed by reverse Northern hybridization. One of these cloned cDNA fragments is a novel cDNA sequence while the other three matched to previously reported cDNA sequences involved in cell growth and migration. Homologous sequences identified to be differentially expressed in normal nasopharyngeal epithelial cells in this study are: human 26 kDa cell surface protein (TAPA-1) mRNA, NF- E2 like basic leucine zipper transcriptional activator and the human bullous pemphigoid antigen. The mRNA differential display is a useful tool to identify candidate genes involved in the pathogenesis of nasopharyngeal carcinoma.en_HK
dc.languageengen_HK
dc.publisherSpandidos Publications. The Journal's web site is located at http://www.spandidos-publications.com/ijo/en_HK
dc.relation.ispartofInternational Journal of Oncologyen_HK
dc.subjectDifferential displayen_HK
dc.subjectNasopharyngeal carcinomaen_HK
dc.subject.meshBase Sequenceen_HK
dc.subject.meshCloning, Molecularen_HK
dc.subject.meshGene Expression Regulation, Neoplasticen_HK
dc.subject.meshHumansen_HK
dc.subject.meshMolecular Sequence Dataen_HK
dc.subject.meshNasopharyngeal Neoplasms - geneticsen_HK
dc.subject.meshNucleotide Mappingen_HK
dc.subject.meshRNA, Messenger - metabolismen_HK
dc.subject.meshTumor Cells, Cultureden_HK
dc.titleIdentification of genes differentially expressed in nasopharyngeal carcinoma by messenger RNA differential displayen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1019-6439&volume=13&spage=85&epage=89&date=1998&atitle=Identification+of+genes+differentially+expressed+in+nasopharyngeal+carcinoma+by+messenger+RNA+differential+displayen_HK
dc.identifier.emailWei, W: hrmswwi@hku.hken_HK
dc.identifier.emailTsao, SW: gswtsao@hkucc.hku.hken_HK
dc.identifier.authorityWei, W=rp00323en_HK
dc.identifier.authorityTsao, SW=rp00399en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid9625807en_HK
dc.identifier.scopuseid_2-s2.0-0031860550en_HK
dc.identifier.hkuros31783en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0031860550&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume13en_HK
dc.identifier.issue1en_HK
dc.identifier.spage85en_HK
dc.identifier.epage89en_HK
dc.identifier.isiWOS:000074212600013-
dc.publisher.placeGreeceen_HK
dc.identifier.scopusauthoridFung, LF=35910298200en_HK
dc.identifier.scopusauthoridYuen, PW=7103124007en_HK
dc.identifier.scopusauthoridWei, W=7403321552en_HK
dc.identifier.scopusauthoridKwan, HS=7103369047en_HK
dc.identifier.scopusauthoridTsao, SW=7102813116en_HK
dc.identifier.issnl1019-6439-

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