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Article: Oxygen-derived free radicals mediate endothelium-dependent contractions in femoral arteries of rats with streptozotocin-induced diabetes
Title | Oxygen-derived free radicals mediate endothelium-dependent contractions in femoral arteries of rats with streptozotocin-induced diabetes |
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Authors | |
Keywords | Endothelium-dependent contractions Endothelium-derived contracting factor Oxidative stress Oxygen-derived free radicals Streptozotocin-induced diabetes |
Issue Date | 2007 |
Publisher | John Wiley & Sons Ltd. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0007-1188&site=1 |
Citation | British Journal Of Pharmacology, 2007, v. 152 n. 7, p. 1033-1041 How to Cite? |
Abstract | Background and purpose: The present experiments were designed to study the contribution of oxygen-derived free radicals to endothelium-dependent contractions in femoral arteries of rats with streptozotocin-induced diabetes. Experimental approach: Rings with and without endothelium were suspended in organ chambers for isometric tension recording. The production of oxygen-derived free radicals in the endothelium was measured with 2′,7′- dichlorodihydrofluorescein diacetate using confocal microscopy. The presence of protein was measured by western blotting. Key results: In the presence of L-NAME, the calcium ionophore A23187 induced larger endothelium-dependent contractions in femoral arteries from diabetic rats. Tiron, catalase, deferoxamine and MnTMPyP, but not superoxide dismutase reduced the response, suggesting that oxygen-derived free radicals are involved in the endothelium-dependent contraction. In the presence of L-NAME, A23187 increased the fluorescence signal in femoral arteries from streptozotocin-treated, but not in those from control rats, confirming that the production of oxygen-derived free radicals contributes to the enhanced endothelium-dependent contractions in diabetes. Exogenous H 2O 2 caused contractions in femoral arterial rings without endothelium which were reduced by deferoxamine, indicating that hydroxyl radicals contract vascular smooth muscle and thus could be an endothelium-derived contracting factor in diabetes. The reduced presence of Mn-SOD and the decreased activity of catalase in femoral arteries from streptozotocin-treated rats demonstrated the presence of a redox abnormality in arteries from rats with diabetes. Conclusions and implications: These findings suggest that the redox abnormality resulting from diabetes increases oxidative stress which facilitates and/or causes endothelium-dependent contractions. © 2007 Nature Publishing Group All rights reserved. |
Persistent Identifier | http://hdl.handle.net/10722/67716 |
ISSN | 2023 Impact Factor: 6.8 2023 SCImago Journal Rankings: 2.119 |
PubMed Central ID | |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Shi, Y | en_HK |
dc.contributor.author | So, KF | en_HK |
dc.contributor.author | Man, RYK | en_HK |
dc.contributor.author | Vanhoutte, PM | en_HK |
dc.date.accessioned | 2010-09-06T05:57:37Z | - |
dc.date.available | 2010-09-06T05:57:37Z | - |
dc.date.issued | 2007 | en_HK |
dc.identifier.citation | British Journal Of Pharmacology, 2007, v. 152 n. 7, p. 1033-1041 | en_HK |
dc.identifier.issn | 0007-1188 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/67716 | - |
dc.description.abstract | Background and purpose: The present experiments were designed to study the contribution of oxygen-derived free radicals to endothelium-dependent contractions in femoral arteries of rats with streptozotocin-induced diabetes. Experimental approach: Rings with and without endothelium were suspended in organ chambers for isometric tension recording. The production of oxygen-derived free radicals in the endothelium was measured with 2′,7′- dichlorodihydrofluorescein diacetate using confocal microscopy. The presence of protein was measured by western blotting. Key results: In the presence of L-NAME, the calcium ionophore A23187 induced larger endothelium-dependent contractions in femoral arteries from diabetic rats. Tiron, catalase, deferoxamine and MnTMPyP, but not superoxide dismutase reduced the response, suggesting that oxygen-derived free radicals are involved in the endothelium-dependent contraction. In the presence of L-NAME, A23187 increased the fluorescence signal in femoral arteries from streptozotocin-treated, but not in those from control rats, confirming that the production of oxygen-derived free radicals contributes to the enhanced endothelium-dependent contractions in diabetes. Exogenous H 2O 2 caused contractions in femoral arterial rings without endothelium which were reduced by deferoxamine, indicating that hydroxyl radicals contract vascular smooth muscle and thus could be an endothelium-derived contracting factor in diabetes. The reduced presence of Mn-SOD and the decreased activity of catalase in femoral arteries from streptozotocin-treated rats demonstrated the presence of a redox abnormality in arteries from rats with diabetes. Conclusions and implications: These findings suggest that the redox abnormality resulting from diabetes increases oxidative stress which facilitates and/or causes endothelium-dependent contractions. © 2007 Nature Publishing Group All rights reserved. | en_HK |
dc.language | eng | en_HK |
dc.publisher | John Wiley & Sons Ltd. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0007-1188&site=1 | en_HK |
dc.relation.ispartof | British Journal of Pharmacology | en_HK |
dc.subject | Endothelium-dependent contractions | en_HK |
dc.subject | Endothelium-derived contracting factor | en_HK |
dc.subject | Oxidative stress | en_HK |
dc.subject | Oxygen-derived free radicals | en_HK |
dc.subject | Streptozotocin-induced diabetes | en_HK |
dc.subject.mesh | Animals | en_HK |
dc.subject.mesh | Antioxidants - pharmacology | en_HK |
dc.subject.mesh | Calcimycin - pharmacology | en_HK |
dc.subject.mesh | Catalase - drug effects - metabolism | en_HK |
dc.subject.mesh | Diabetes Mellitus, Experimental - chemically induced - metabolism - physiopathology | en_HK |
dc.subject.mesh | Disease Models, Animal | en_HK |
dc.subject.mesh | Dose-Response Relationship, Drug | en_HK |
dc.subject.mesh | Endothelium, Vascular - drug effects - metabolism | en_HK |
dc.subject.mesh | Enzyme Activation - drug effects | en_HK |
dc.subject.mesh | Enzyme Inhibitors - pharmacology | en_HK |
dc.subject.mesh | Femoral Artery - drug effects - metabolism - physiopathology | en_HK |
dc.subject.mesh | Hydrogen Peroxide - antagonists & inhibitors - pharmacology | en_HK |
dc.subject.mesh | Male | en_HK |
dc.subject.mesh | Microscopy, Confocal - methods | en_HK |
dc.subject.mesh | Organ Culture Techniques | en_HK |
dc.subject.mesh | Oxidants - pharmacology | en_HK |
dc.subject.mesh | Oxidation-Reduction | en_HK |
dc.subject.mesh | Rats | en_HK |
dc.subject.mesh | Rats, Sprague-Dawley | en_HK |
dc.subject.mesh | Reactive Oxygen Species - metabolism | en_HK |
dc.subject.mesh | Signal Transduction - drug effects | en_HK |
dc.subject.mesh | Streptozocin | en_HK |
dc.subject.mesh | Superoxide Dismutase - drug effects - metabolism | en_HK |
dc.subject.mesh | Vasoconstriction - drug effects | en_HK |
dc.title | Oxygen-derived free radicals mediate endothelium-dependent contractions in femoral arteries of rats with streptozotocin-induced diabetes | en_HK |
dc.type | Article | en_HK |
dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0007-1188&volume=152&spage=1033&epage=1041&date=2007&atitle=Oxygen-derived+free+radicals+mediate+endothelium-dependent+contractions+in+femoral+arteries+of+rats+with+streptozotocin-induced+diabetes | en_HK |
dc.identifier.email | So, KF: hrmaskf@hkucc.hku.hk | en_HK |
dc.identifier.email | Man, RYK: rykman@hkucc.hku.hk | en_HK |
dc.identifier.email | Vanhoutte, PM: vanhoutt@hku.hk | en_HK |
dc.identifier.authority | So, KF=rp00329 | en_HK |
dc.identifier.authority | Man, RYK=rp00236 | en_HK |
dc.identifier.authority | Vanhoutte, PM=rp00238 | en_HK |
dc.description.nature | link_to_OA_fulltext | - |
dc.identifier.doi | 10.1038/sj.bjp.0707439 | en_HK |
dc.identifier.pmid | 17767168 | - |
dc.identifier.pmcid | PMC2095103 | - |
dc.identifier.scopus | eid_2-s2.0-36448929845 | en_HK |
dc.identifier.hkuros | 151173 | en_HK |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-36448929845&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 152 | en_HK |
dc.identifier.issue | 7 | en_HK |
dc.identifier.spage | 1033 | en_HK |
dc.identifier.epage | 1041 | en_HK |
dc.identifier.isi | WOS:000251139600007 | - |
dc.publisher.place | United Kingdom | en_HK |
dc.identifier.scopusauthorid | Shi, Y=7404964959 | en_HK |
dc.identifier.scopusauthorid | So, KF=34668391300 | en_HK |
dc.identifier.scopusauthorid | Man, RYK=7004986435 | en_HK |
dc.identifier.scopusauthorid | Vanhoutte, PM=7202304247 | en_HK |
dc.identifier.issnl | 0007-1188 | - |