File Download
Links for fulltext
(May Require Subscription)
- Publisher Website: 10.1016/j.neulet.2009.12.028
- Scopus: eid_2-s2.0-73849147183
- PMID: 20026175
- WOS: WOS:000274722100017
- Find via
Supplementary
- Citations:
- Appears in Collections:
Article: A pro-drug of the green tea polyphenol (-)-epigallocatechin-3-gallate (EGCG) prevents differentiated SH-SY5Y cells from toxicity induced by 6-hydroxydopamine
Title | A pro-drug of the green tea polyphenol (-)-epigallocatechin-3-gallate (EGCG) prevents differentiated SH-SY5Y cells from toxicity induced by 6-hydroxydopamine | ||||||||
---|---|---|---|---|---|---|---|---|---|
Authors | |||||||||
Keywords | 6-Hydroxydopamine Green tea polyphenols/EGCG Neuroprotection Prodrug/pEGCG | ||||||||
Issue Date | 2010 | ||||||||
Publisher | Elsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/neulet | ||||||||
Citation | Neuroscience Letters, 2010, v. 469 n. 3, p. 360-364 How to Cite? | ||||||||
Abstract | Regular consumption of green tea benefits people in prevention from cardiovascular disorders, obesity as well as neurodegenerative diseases. (-)-Epigallocatechin-3-gallate (EGCG) is regarded as the most biologically active catechin in green tea. However, the stability and bioavailability of EGCG are restricted. The purpose of the present study was to investigate whether a pro-drug, a fully acetylated EGCG (pEGCG), could be more effective in neuroprotection in Parkinsonism mimic cellular model. Retinoic acid (RA)-differentiated neuroblastoma SH-SY5Y cells were pre-treated with different concentrations of EGCG and pEGCG for 30 min and followed by incubation of 25 μM 6-hydroxydopamine (6-OHDA) for 24 h. We found that a broad dosage range of pEGCG (from 0.1 to 10 μM) could significantly reduce lactate dehydrogenase release. Likewise, 10 μM of pEGCG was effective in reducing caspase-3 activity, while EGCG at all concentrations tested in the model failed to attenuate caspase-3 activity induced by 6-OHDA. Furthermore, Western-blot analysis showed that Akt could be one of the specific signaling pathways stimulated by pEGCG in neuroprotection. It was demonstrated that 25 μM of 6-OHDA significantly suppressed the phosphorylation level of Akt. Only pEGCG at 10 μM markedly increased its phosphorylation level compared to 6-OHDA alone. Taken together, as pEGCG has higher stability and bioavailbility for further investigation, it could be a potential neuroprotective agent and our current findings may offer certain clues for optimizing its application in future. © 2009 Elsevier Ireland Ltd. All rights reserved. | ||||||||
Persistent Identifier | http://hdl.handle.net/10722/67956 | ||||||||
ISSN | 2023 Impact Factor: 2.5 2023 SCImago Journal Rankings: 0.745 | ||||||||
ISI Accession Number ID |
Funding Information: The study is supported by HKU Strategic Research Theme on Drug Discovery, Area of Excellence on Institute of Molecular Technology for Drug Discovery and Synthesis (AoE/P-10/01), and The Hong Kong Polytechnic University Area of Strategic Development Fund. YSH is supported by a postdoctoral fellowship. | ||||||||
References | |||||||||
Grants |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Chao, J | en_HK |
dc.contributor.author | Lau, WKW | en_HK |
dc.contributor.author | Huie, MJ | en_HK |
dc.contributor.author | Ho, YS | en_HK |
dc.contributor.author | Yu, MS | en_HK |
dc.contributor.author | Lai, CSW | en_HK |
dc.contributor.author | Wang, M | en_HK |
dc.contributor.author | Yuen, WH | en_HK |
dc.contributor.author | Lam, WH | en_HK |
dc.contributor.author | Chan, TH | en_HK |
dc.contributor.author | Chang, RCC | en_HK |
dc.date.accessioned | 2010-09-06T05:59:48Z | - |
dc.date.available | 2010-09-06T05:59:48Z | - |
dc.date.issued | 2010 | en_HK |
dc.identifier.citation | Neuroscience Letters, 2010, v. 469 n. 3, p. 360-364 | en_HK |
dc.identifier.issn | 0304-3940 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/67956 | - |
dc.description.abstract | Regular consumption of green tea benefits people in prevention from cardiovascular disorders, obesity as well as neurodegenerative diseases. (-)-Epigallocatechin-3-gallate (EGCG) is regarded as the most biologically active catechin in green tea. However, the stability and bioavailability of EGCG are restricted. The purpose of the present study was to investigate whether a pro-drug, a fully acetylated EGCG (pEGCG), could be more effective in neuroprotection in Parkinsonism mimic cellular model. Retinoic acid (RA)-differentiated neuroblastoma SH-SY5Y cells were pre-treated with different concentrations of EGCG and pEGCG for 30 min and followed by incubation of 25 μM 6-hydroxydopamine (6-OHDA) for 24 h. We found that a broad dosage range of pEGCG (from 0.1 to 10 μM) could significantly reduce lactate dehydrogenase release. Likewise, 10 μM of pEGCG was effective in reducing caspase-3 activity, while EGCG at all concentrations tested in the model failed to attenuate caspase-3 activity induced by 6-OHDA. Furthermore, Western-blot analysis showed that Akt could be one of the specific signaling pathways stimulated by pEGCG in neuroprotection. It was demonstrated that 25 μM of 6-OHDA significantly suppressed the phosphorylation level of Akt. Only pEGCG at 10 μM markedly increased its phosphorylation level compared to 6-OHDA alone. Taken together, as pEGCG has higher stability and bioavailbility for further investigation, it could be a potential neuroprotective agent and our current findings may offer certain clues for optimizing its application in future. © 2009 Elsevier Ireland Ltd. All rights reserved. | en_HK |
dc.language | eng | en_HK |
dc.publisher | Elsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/neulet | en_HK |
dc.relation.ispartof | Neuroscience Letters | en_HK |
dc.rights | Neuroscience Letters. Copyright © Elsevier Ireland Ltd. | en_HK |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.subject | 6-Hydroxydopamine | en_HK |
dc.subject | Green tea polyphenols/EGCG | en_HK |
dc.subject | Neuroprotection | en_HK |
dc.subject | Prodrug/pEGCG | en_HK |
dc.subject.mesh | Catechin - administration and dosage - analogs and derivatives - chemistry - pharmacology | - |
dc.subject.mesh | Central Nervous System Agents - administration and dosage - toxicity | - |
dc.subject.mesh | Neurons - cytology - drug effects - metabolism | - |
dc.subject.mesh | Neuroprotective Agents - administration and dosage - chemistry - pharmacology | - |
dc.subject.mesh | Oxidopamine - administration and dosage - toxicity | - |
dc.title | A pro-drug of the green tea polyphenol (-)-epigallocatechin-3-gallate (EGCG) prevents differentiated SH-SY5Y cells from toxicity induced by 6-hydroxydopamine | en_HK |
dc.type | Article | en_HK |
dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0304-3940&volume=469&issue=3&spage=360&epage=364&date=2010&atitle=A+pro-drug+of+the+green+tea+polyphenol+(-)-epigallocatechin-3-gallate+(EGCG)+prevents+differentiated+SH-SY5Y+cells+from+toxicity+induced+by+6-hydroxydopamine | en_HK |
dc.identifier.email | Wang, M: mfwang@hku.hk | en_HK |
dc.identifier.email | Chang, RCC: rccchang@hku.hk | en_HK |
dc.identifier.authority | Wang, M=rp00800 | en_HK |
dc.identifier.authority | Chang, RCC=rp00470 | en_HK |
dc.description.nature | postprint | - |
dc.identifier.doi | 10.1016/j.neulet.2009.12.028 | en_HK |
dc.identifier.pmid | 20026175 | - |
dc.identifier.scopus | eid_2-s2.0-73849147183 | en_HK |
dc.identifier.hkuros | 169237 | en_HK |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-73849147183&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 469 | en_HK |
dc.identifier.issue | 3 | en_HK |
dc.identifier.spage | 360 | en_HK |
dc.identifier.epage | 364 | en_HK |
dc.identifier.isi | WOS:000274722100017 | - |
dc.publisher.place | Ireland | en_HK |
dc.relation.project | Institute of molecular technology for drug discovery and synthesis | - |
dc.identifier.scopusauthorid | Chao, J=24558959000 | en_HK |
dc.identifier.scopusauthorid | Lau, WKW=35292424400 | en_HK |
dc.identifier.scopusauthorid | Huie, MJ=35867164800 | en_HK |
dc.identifier.scopusauthorid | Ho, YS=14031513600 | en_HK |
dc.identifier.scopusauthorid | Yu, MS=35346047600 | en_HK |
dc.identifier.scopusauthorid | Lai, CSW=26022547000 | en_HK |
dc.identifier.scopusauthorid | Wang, M=7406691844 | en_HK |
dc.identifier.scopusauthorid | Yuen, WH=7102761282 | en_HK |
dc.identifier.scopusauthorid | Lam, WH=26643561300 | en_HK |
dc.identifier.scopusauthorid | Chan, TH=35291257900 | en_HK |
dc.identifier.scopusauthorid | Chang, RCC=7403713410 | en_HK |
dc.identifier.citeulike | 6476727 | - |
dc.identifier.issnl | 0304-3940 | - |