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Article: In vitro evaluation of alginate encapsulated adipose-tissue stromal cells for use as injectable bone graft substitute

TitleIn vitro evaluation of alginate encapsulated adipose-tissue stromal cells for use as injectable bone graft substitute
Authors
Abbah, SALu, WWChan, DCheung, KMCLiu, WGZhao, FLi, ZYLeong, JCYLuk, KDK
KeywordsAdipose-tissue stromal cells
Bone tissue engineering
Ca-alginate
Cell transplantation
Microcapsules
Issue Date2006
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/description
Citation
Biochemical And Biophysical Research Communications, 2006, v. 347 n. 1, p. 185-191 How to Cite?
DOI: http://dx.doi.org/10.1016/j.bbrc.2006.06.072
AbstractThis study aims to investigate the survival and osteogenic behavior of murine-derived adipose-tissue stromal cells (ATSCs) encapsulated in alginate microcapsules thereby instigating further studies in this cell delivery strategy for in vivo osteogenesis. Cell viability was quantified using a tetrazolium-based assay and osteogenic differentiation was evaluated by both alkaline-phosphatase (ALP) histochemistry and osteocalcin mRNA analysis. Following microencapsulation, cell numbers increased from 3.9 × 103 on day 1 to 7.8 × 103 on day 7 and maintained excellent viability in the course of 21-day culture. ALP was 6.9, 5.5, and 3.2 times higher than monolayer cultures on days 7, 14, and 21, respectively. In addition, osteocalcin mRNA was detectable in encapsulated cultures earlier (day 14) than monolayer cultures. We conclude that alginate microcapsules can act as three-dimensional matrix for ATSC proliferation and has potential for use as injectable, biodegradable scaffold in bone tissue engineering. © 2006 Elsevier Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/68216
ISSN
0006-291X
2021 Impact Factor: 3.322
2020 SCImago Journal Rankings: 0.998
ISI Accession Number ID
WOS:000239198000025
References
References in Scopus

 

DC FieldValueLanguage
dc.contributor.authorAbbah, SAen_HK
dc.contributor.authorLu, WWen_HK
dc.contributor.authorChan, Den_HK
dc.contributor.authorCheung, KMCen_HK
dc.contributor.authorLiu, WGen_HK
dc.contributor.authorZhao, Fen_HK
dc.contributor.authorLi, ZYen_HK
dc.contributor.authorLeong, JCYen_HK
dc.contributor.authorLuk, KDKen_HK
dc.date.accessioned2010-09-06T06:02:28Z-
dc.date.available2010-09-06T06:02:28Z-
dc.date.issued2006en_HK
dc.identifier.citationBiochemical And Biophysical Research Communications, 2006, v. 347 n. 1, p. 185-191en_HK
dc.identifier.issn0006-291Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/68216-
dc.description.abstractThis study aims to investigate the survival and osteogenic behavior of murine-derived adipose-tissue stromal cells (ATSCs) encapsulated in alginate microcapsules thereby instigating further studies in this cell delivery strategy for in vivo osteogenesis. Cell viability was quantified using a tetrazolium-based assay and osteogenic differentiation was evaluated by both alkaline-phosphatase (ALP) histochemistry and osteocalcin mRNA analysis. Following microencapsulation, cell numbers increased from 3.9 × 103 on day 1 to 7.8 × 103 on day 7 and maintained excellent viability in the course of 21-day culture. ALP was 6.9, 5.5, and 3.2 times higher than monolayer cultures on days 7, 14, and 21, respectively. In addition, osteocalcin mRNA was detectable in encapsulated cultures earlier (day 14) than monolayer cultures. We conclude that alginate microcapsules can act as three-dimensional matrix for ATSC proliferation and has potential for use as injectable, biodegradable scaffold in bone tissue engineering. © 2006 Elsevier Inc. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/descriptionen_HK
dc.relation.ispartofBiochemical and Biophysical Research Communicationsen_HK
dc.subjectAdipose-tissue stromal cellsen_HK
dc.subjectBone tissue engineeringen_HK
dc.subjectCa-alginateen_HK
dc.subjectCell transplantationen_HK
dc.subjectMicrocapsulesen_HK
dc.subject.meshAdipocytes - cytology - transplantationen_HK
dc.subject.meshAlginates - chemistryen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshBiocompatible Materials - chemistryen_HK
dc.subject.meshBone Substitutes - administration & dosage - chemistryen_HK
dc.subject.meshBone Transplantation - methodsen_HK
dc.subject.meshCell Culture Techniques - methodsen_HK
dc.subject.meshCell Differentiationen_HK
dc.subject.meshCell Transplantation - methodsen_HK
dc.subject.meshCells, Cultureden_HK
dc.subject.meshCoated Materials, Biocompatible - chemistryen_HK
dc.subject.meshGlucuronic Acid - chemistryen_HK
dc.subject.meshHexuronic Acids - chemistryen_HK
dc.subject.meshInjections - methodsen_HK
dc.subject.meshMiceen_HK
dc.subject.meshMice, Transgenicen_HK
dc.subject.meshOsteoblasts - cytologyen_HK
dc.subject.meshStromal Cells - cytology - transplantationen_HK
dc.subject.meshTissue Engineering - methodsen_HK
dc.titleIn vitro evaluation of alginate encapsulated adipose-tissue stromal cells for use as injectable bone graft substituteen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0006-291X&volume=347&issue=1&spage=185&epage=91&date=2006&atitle=In+vitro+evaluation+of+alginate+encapsulated+adipose-tissue+stromal+cells+for+use+as+injectable+bone+graft+substitute.en_HK
dc.identifier.emailLu, WW:wwlu@hku.hken_HK
dc.identifier.emailChan, D:chand@hkucc.hku.hken_HK
dc.identifier.emailCheung, KMC:cheungmc@hku.hken_HK
dc.identifier.emailLuk, KDK:hcm21000@hku.hken_HK
dc.identifier.authorityLu, WW=rp00411en_HK
dc.identifier.authorityChan, D=rp00540en_HK
dc.identifier.authorityCheung, KMC=rp00387en_HK
dc.identifier.authorityLuk, KDK=rp00333en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.bbrc.2006.06.072en_HK
dc.identifier.pmid16815293-
dc.identifier.scopuseid_2-s2.0-33745754926en_HK
dc.identifier.hkuros123822en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33745754926&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume347en_HK
dc.identifier.issue1en_HK
dc.identifier.spage185en_HK
dc.identifier.epage191en_HK
dc.identifier.isiWOS:000239198000025-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridAbbah, SA=14032930600en_HK
dc.identifier.scopusauthoridLu, WW=7404215221en_HK
dc.identifier.scopusauthoridChan, D=7402216545en_HK
dc.identifier.scopusauthoridCheung, KMC=7402406754en_HK
dc.identifier.scopusauthoridLiu, WG=35269689700en_HK
dc.identifier.scopusauthoridZhao, F=36040646100en_HK
dc.identifier.scopusauthoridLi, ZY=35784563200en_HK
dc.identifier.scopusauthoridLeong, JCY=35560782200en_HK
dc.identifier.scopusauthoridLuk, KDK=7201921573en_HK
dc.identifier.issnl0006-291X-

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